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Navegando por Assunto "Interleucina"

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    Determination of strains of Helicobacter pylori and of polymorphism in the interleukin-8 gene in patients with stomach cancer
    (2011) VINAGRE, Ruth Maria Dias Ferreira; CORVELO, Tereza Cristina de Oliveira; ARNAUD, Vanda Catão; LEITE, Ana Claudia Klautau; BARILE, Katarine Antonia dos Santos; MARTINS, Luisa Caricio
    CONTEXT: Gastric neoplasia is the second most common cause of death by cancer in the world and H. pylori is classified as a type I human carcinogen by the World Health Organization. However, despite the high prevalence of infection by H. pylori around the world, less than 3% of individuals carrying the bacteria develop gastric neoplasias. Such a fact indicates that evolution towards malignancy may be associated with bacterial factors in the host and the environment. OBJECTIVES: To investigate the association between polymorphism in the region promoting the IL-8 (-251) gene and the H. pylori genotype, based on the vacA alleles and the presence of the cagA gene, using clinical and histopathological data. METHODS: In a prospective study, a total of 102 patients with stomach cancer and 103 healthy volunteers were analysed. Polymorphism in interleukin 8 (-251) was determined by the PCR-restriction fragment length polymorphism reaction and sequencing. PCR was used for genotyping the vacA alleles and the cagA in the bacterial strains PCR. Gastric biopsies were histologically assessed. RESULTS: The H. pylori serology was positive for 101 (99%) of all patients analysed, and 98 (97%) of them were colonized by only one strain. In patients with monoinfection, 82 (84%) of the bacterial strains observed had the s1b/m1 genotype. The cagA gene was detected in 74 (73%) of patients infected by H. pylori. The presence of the cagA gene was demonstrated as associated with the presence of the s1b/m1 genotype of the vacA gene (P = 0.002). As for polymorphism in the interleukin 8 (-251) gene we observed that the AA (P = 0.026) and AT (P = 0.005) genotypes were most frequent in the group of patients with gastric adenocarcinoma. By comparing the different types of isolated bacterial strains with the interleukin -8 (-251) and the histopathological data we observed that carriers of the A allele (AT and AA) infected by virulent strains (m1s1 cagA+) demonstrated a greater risk of presenting a degree of inflammation (OR = 24.75 CI 95% 2.29-267.20 P = 0.004) and increased neutrophilic activity (OR = 28.71 CI 95% 2.62-314 P = 0.002) in the gastric mucosa. CONCLUSION: Our results demonstrate that the interaction between polymorphism in the interleukin -8 (-251) gene, particularly with carriers of the A allele and the infecting type of H. pylori strain (s1m1 cagA positive) performs an important function in development of gastric adenocarcinoma.
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    IL-2, IL-5, TNF-α and IFN-γ mRNA expression in epidermal keratinocytes of systemic lupus erythematosus skin lesions
    (2011) CARNEIRO, José Ronaldo Matos; FUZII, Hellen Thais; KAYSER, Cristiane; ALBERTO, Fernando Lopes; SOARES, Fernando Augusto; SATO, Emília Inoue; ANDRADE, Luis Eduardo Coelho
    OBJECTIVE: To analyze cytokine gene expression in keratinocytes from patients with systemic lupus erythematosus (SLE). INTRODUCTION: Keratinocytes represent 95% of epidermal cells and can secrete several cytokines. METHODS: Keratinocytes were obtained by laser microdissection from 21 patients with SLE (10 discoid and 11 acute lesions) at involved and uninvolved sites. All patients were receiving a low/moderate prednisone dose and 18 were receiving chloroquine diphosphate. IL-2, IL-5, TNF-α and IFN-γ gene expression was evaluated by real-time PCR and expressed as the ratio (R) to a pool of skin samples from 12 healthy volunteers. RESULTS: Heterogeneity in cytokine gene expression was found among patients with SLE. Eighteen of 38 valid SLE samples (47%) presented overexpression (R>1) of at least one cytokine. Lesional skin samples tended to show higher cytokine expression than samples from uninvolved skin (p = 0.06). IL-5 and IFN-γ were the most commonly overexpressed cytokines. Samples with cytokine overexpression corresponded to more extensive and severe lesions. Prednisone dose did not differ between samples without cytokine overexpression (15.71±3.45 mg/day) and those with overexpressed cytokines (12.68±5.41 mg/day) (p = 0.216). Samples from all patients not receiving diphosphate chloroquine had at least one overexpressed cytokine. CONCLUSIONS: The heterogeneous keratinocyte cytokine gene expression reflects the complex immunological and inflammatory background in SLE. Patients with severe/extensive skin lesions showed a higher frequency of cytokine gene overexpression. Increased IFN-γ and IL-5 expression suggests that Th1 and Th2 cells are involved in SLE skin inflammation. The possibility that prednisone and antimalarial drugs may have contributed to low cytokine gene expression in some samples cannot be ruled out.
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    Níveis de mercúrio, prolactina e interleucina 10 em mulheres em idade reprodutiva e puérperas dos municípios de Itaituba e Ananindeua, Pará
    (Universidade Federal do Pará, 2012-05-02) JESUS, Iracina Maura de; DINIZ, Domingos Luiz Wanderley Picanço; http://lattes.cnpq.br/9601463988942971
    The ability of interfering in the immunological and endocrine organic systems has been attributed to the mercury (Hg), besides the nervous and renal systems frequently affected by this toxicant agent. Women in fertile age or pregnant constitute a vulnerable group for those effects, in relation to themselves and their fetus. The mercury exposure was assessed as well as the prolactin (PRL) and interleukin-10 (IL-10) levels in 144 women (in the post-partum and about one year later) of Itaituba, area under environmental Hg impact and in women of the metropolitan area of Belém, most of all from Ananindeua, area without known Hg impact (156 puerperal women and 156 non-puerperal). Total Hg (Hg-t) analyses in whole blood were carried out by Atomic Absorption Spectrometry Cold Vapor. Serum analyses of PRL were made by Enzyme Immunoassay with final detection by fluorescence and IL-10 serum analyses were accomplished by Enzyme Immunoassay of Solid Phase. Demographic and epidemiological data were obtained through semi-structured questionnaire. Puerperal women of Itaituba presented average of Hg-t, PRL and IL-10 of 13.93 μg/l, 276.20 ng/ml and 39.54 pg/ml, respectively. Puerperal women of Ananindeua presented respective Hg-t, PRL and IL-10 averages of 3.76 μg/l, 337.70 ng/ml and 4.90 pg/ml. Non-puerperal women of Itaituba presented Hg-t mean of 12.68 μg/l, PRL mean of 30.75 ng/ml and IL-10 mean of 14.20 pg/ml. Mean of Hg-t, PRL and IL-10 in non-puerperal women from Ananindeua were of 2.73 μg/l, 17.07 ng/ml and 1.49 pg/ml, respectively. Levels of Hg-t, PRL and IL-10 were higher in Itaituba (p<0.0001), except in PRL levels of puerperal women, higher in Ananindeua. Similar Hg levels in women of Itaituba in two assessment (p=0.7056) and positive correlation suggest continuity of exposure (r=0.4736, p<0.0001). The main predictor variable of mercury levels was the fish consumption in the linear and logistic multiple regression models. Parity and IL-10 levels were positively associated with PRL in puerperal women of Itaituba while newborn weight and IL-10 levels presented positive association with PRL in puerperal women of Ananindeua. IL-10 was negatively associated with PRL in non-puerperal women from Itaituba (p=0.0270) and positive association in Ananindeua (p=0.0266). Levels of Hg-t showed negative association with PRL in puerperal women and positive association with working in garimpo (p=0.0173) (the last one was also important for non-puerperal women) in Itaituba, according logistic models. IL-10 was positively associated with recent morbidity in puerperal women of Itaituba (p=0.0210), negatively with ingestion of alcoholic beverages (p=0.0178) and positively with working in garimpo in non-puerperal women (p=0.0199). The chronic Hg exposure of women from Itaituba, difference among the Hg, PRL and IL-10 levels in exposed women compared with non exposed group and association with relevant epidemiological variables, suggest the possibility of Hg impacts in the women's immunoendocrine system in Itaituba, calling attention for the health surveillance of this population and the possible use of assessment biomarkers as PRL.
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    Polimorfismo do gene da interleucina IL-1B e sua associação com o risco ao desenvolvimento do câncer gástrico em uma população do norte do Brasil
    (Universidade Federal do Pará, 2016-12-22) CASTRO, Yaisa Gomes de; SANTOS, Ândrea Kely Campos Ribeiro dos; http://lattes.cnpq.br/3899534338451625
    Cancer is understood as a set of diseases with similar characteristics, but with great heterogeneity that occurs in a random manner and covers both tumor and inflammatory and immune cells. Gastric tumors, in Brazil and notably in the State of Pará, have a high incidence. In general, gastric cancer has a multifactorial etiology. Communication and cellular signaling that regulate the immune system are facilitated by interleukins that represent small, specific proteins, have diverse functions, they regulate transcription factors, role genes, inflammation, differentiation, proliferation, and secretion of antibodies. Single polymorphism nucleotide, in specific IL-1B proinflammatory interleukin gene, is associated with the immune response to H. pylori infection. Thefore variations within the IL-1 family genes were associated with susceptibility to the development of gastric cancer. In this case-control study, we investigated whether the polymorphisms IL-1BF1 (rs16944) and IL-1BE1 (rs1143627) are associated with the risk of developing gastric cancer in a population from the north of Brazil; Compared to their respective genotypes, defined haplotypes and these related to ancestry and their rates. SNPs were genotyped by VIC / FAM (Real Time PCR, Fluorescent, Life Technologies, CA, USA) labeled probes. The biostatistical analyzes showed that for the demographic variables, there were significant differences between the groups in European and African ancestry. The distribution of the genotypic, allelic and haplotype frequencies of the IL-1B gene was not statistically significant between the groups. More comprehensive studies and analyzes are needed to help understand better why these polymorphisms in this population do not appear to be associated with the development of the disease in question.
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