Navegando por Assunto "Interleucinas"

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Acesso aberto (Open Access)
Análise de citocinas no soro de pacientes com câncer gástrico
(Universidade Federal do Pará, 2017-08-16) HAGE, Pedro Antônio Mufarrej; CALCAGNO, Danielle Queiroz; http://lattes.cnpq.br/1326603355062154
Despite the reduction in the worldwide incidence of gastric cancer, this neoplasm remains the second largest cause of cancer death in the world. Late diagnosis occurs mainly due to the absence of symptoms or the presence of non-specific symptoms in the early stages of the disease. In this case, few effective therapeutic options are available, resulting in high rates of morbidity and mortality. The continuous study of new strategies for the early diagnosis, definition of the prognosis and identification of new therapeutic methods is of great interest in this neoplastic type. In the present study was quantified inflammatory proteins candidate to biomarkers in the serum of 19 patients with gastric adenocarcinoma before surgical resection and 13 healthy individuals as control. The methodology used for quantification of proteins was the MAGPIX system and panels of cancer biomarkers inventoried by the manufacturer (Bio-Plex Pro Human Pro-Cytokine, Chemokine and Growth Factors). In the comparison between patients with gastric adenocarcinoma and control group was observed the levels of IL-1β, IL-1RA, IL-2, IL-6, IL-8, IL-10, IL-12, IL-15, G-CSF, GM-CSF, IFN-γ, MIP1α, RANTES and VEGF were higher in serum of gastric adenocarcinoma patients. According clinicopathological characteristics, was observed elevated levels of IL-5, IL-7, IL-10 and IL-17A in diffuse-type gastric adenocarcinoma in relation intestinal-type. In addition, the association of the expression of the cytokines studied with overall survival (OS) and relapse-free survival (PFS) were performed using the KM Plotter Online Tool. Overexpression of G-CSF, GM-CSF and VEGF in tumor was associated with lower OS and PFS of gastric cancer patients. However, the overexpression of IL-10 was associated only with PFS. Thus, we can conclude that the IL-1β, IL-1RA, IL-2, IL-6, IL-8, IL-10, IL-12, IL-15, G-CSF, GM-CSF, IFN-γ, MIP1α, RANTES and VEGF cytokines are potential biomarkers of gastric cancer, and diffuse-type adenocarcinoma can related to a greater inflammatory response than intestinal-type. Survival analysis suggests that elevated levels of IL-10, G-CSF, GM-CSF and VEGF in serum are potential biomarkers of prognosis in gastric cancerpatients.
Acesso aberto (Open Access)
Modelo murino do espectro autista empregando o ácido valproico durante a gravidez: mudanças comportamentais e citocinas pró-inflamatórias
(Universidade Federal do Pará, 2018-10-31) SOUZA, Dilza Nazaré Colares de; DINIZ, Cristovam Wanderley Picanço; http://lattes.cnpq.br/2014918752636286; DINIZ, Daniel Guerreiro; http://lattes.cnpq.br/3269424921125406
The present study evaluated, in behavioral tests, the exploratory and locomotor activities of young adult BALB/c mice that were exposed to valproic acid during pregnancy and measured their proinflammatory cytokines concentration in peripheral blood. To this end, we exposure females to males and on day 12.5 of gestation, females received 0.2 mL of VPA diluted in saline (600 mg / kg body weight) or equal volume of saline solution. The pups were weaned on the 21st postnatal day and the males were kept either in the impoverished environment of standard laboratory cages (IE) or in an enriched cage (EE). Four independent experimental groups according to experimental condition and environment (Ctrl / EE, Ctrl / IE, VPA / EE, VPA / IE) were organized. At 5 months of age, all animals were submitted to Open Field (OF), Episodic Memory (EM), Burrowing and Elevated Plus Maze (EPM) tests and had their peripheral blood collected and then perfused with saline followed by aldehyde fixatives. Two-way ANOVA revealed significant influences of experimental condition (VPA vs. Saline) and environment (EE vs IE) on behavioral outcomes and on proinflammatory cytokines peripheral concentrations. In the open field, the valproic acid groups, regardless of the environment where they were kept, reversed the natural tendency to avoid the center of the arena, F (1,54) = 5.59, p = 0.022. Similarly, in the elevated plus maze, the valproic acid groups, independent of the environment where the animals were kept, showed a significant influence on the time spent in the central platform, reducing it significantly, F (1,51) = 7.57, p = 0.0082. Two-way ANOVA also demonstrated a significant influence of the experimental condition (VPA vs. Saline) on the immune response reducing IL-1β, F (1.49) = 26.24, p <0.0001 and increasing IL-6, F (1.46) = 16.96, p = 0.0002 of the valproic acid groups. BALB/c mice exposed to valproic acid during pregnancy shows significant changes on their behavior to explore novel environments and to assess risk at adulthood, and this is associated with proinflammatory cytokines peripheral changes. Somatosensory and cognitive stimulation of environmental enrichment seems to be not enough to reverse it.