Navegando por Assunto "Jambu"

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Acesso aberto (Open Access)
Avaliação do teor de espilatol no ciclo de cultura de duas cultivares de Aemella oleracea (L.) R. K. Jansen em extratos obtidos por entração supercritica
(Universidade Federal do Pará, 2018-12-10) SILVA, Ana Paula de Souza e; FERREIRA, Gracialda Costa; http://lattes.cnpq.br/4250668524181387; CARVALHO JÚNIOR, Raul Nunes de; http://lattes.cnpq.br/5544305606838748; https://orcid.org/0000-0002-4433-6580
Acmella oleracea (L.) R. K. Jansen is a species native to South America, popularly known as jambu, and has been consumed for many years as an ornamental, medicinal, and food plant. To obtain espilantol, the main bioactive compound of this species, various extraction techniques have been used in order to obtain the isolated substance, since it is not commercially available. Among the techniques, supercritical carbon dioxide extraction stands out as it showed high selectivity for espilantol, with yields above 50%, resulting in a purity above 90% of the isolated compound. The objective of this work was to evaluate the influence of rainfall and harvest time on the yield of spilanthol with supercritical CO2 in two cultivars of Acmella oleracea (L.) R. K. Jansen flowers, as well as to evaluate their pharmacological effect. For this purpose, the following procedures were performed: raw material characterization; optimization of the process variables (flow and time); obtaining of extracts by supercritical extraction; determining of the process cost on an analytical scale; determination of the total phenolic compounds concentration in the extracts by Folin-Ciocalteu method; spilanthol content quantification in the extracts by gas chromatography coupled to mass spectrometry, and evaluation of its antinociceptive in vivo activity. The main results were: the mineral analysis indicated high levels of iron, calcium, zinc, and magnesium; the process optimization resulted in a CO2 use decrease from 955,8g to 477,9g, and reduced extraction costs from R$ 20.47 to R$ 11.45. The higher espilantol content was 29.22%, while the highest phenolic content was 43.04%. The flowers of both Acmella oleracea cultivars were considered good sources of iron, calcium, zinc, and magnesium. The highest values of extract yield, spilanthol content and total phenolic compounds were obtained in the rainy season and in the shortest harvest times. Between the two cultivars evaluated, no significant statistical differences were found.
Acesso aberto (Open Access)
Desenvolvimento de bebida fermentada análoga a kombucha, à base de inflorescências de jambu (acmella oleracea), e monitoramento de compostos bioativos
(Universidade Federal do Pará, 2023-12-19) BARROS, Vinícius Costa; BOTELHO, Vanessa Albres; http://lattes.cnpq.br/0992385832433182; CHISTÉ, Renan Campos; http://lattes.cnpq.br/0583058299891937; https://orcid.org/0000-0002-4549-3297
Kombucha is a fermented product that is increasingly expanding in relation to its consumption and the advancement of its research due to claims of its correlated health benefits, arising from its compounds generated in fermentation. Therefore, the following research aimed to corroborate these advances by developing an analogue to the fermented one using jambu (Acmella oleracea) as substrate, three formulations of the drink were developed in which in the first stage the pH values, total acidity were monitored for 7 days, total soluble solids, total and reducing sugars, antioxidant capacity, in addition to total phenolic compounds and flavonoids. After the product was ready, the same parameters, as in the first fermentation, were observed with the addition of volatile and fixed acidity, density, profile of phenolic compounds, microbiological and sensory analyses. The final products agreed with the regulated standard for kombuchas (IN 41 MAPA, Brazil), being microbiologically safe, and the formulation with 75% of jambu addition was the one with the highest sensorial preference as well as purchasing potential. In general, during the first fermentation, the formulation with 50% of jambu addition showed the best performance for phenolic compounds, flavonoids, and antioxidant capacity, requiring new future stability studies of these compounds to be available in ready-made products.
Acesso aberto (Open Access)
Investigação do efeito ictiotóxico do extrato etanólico da raíz de Spilanthes acmella (jambú) em zebrafish através da análise eletrofisiológica e comportamental
(Universidade Federal do Pará, 2013-12-09) RIBEIRO, Layza Costa; ROCHA, Fernando Allan de Farias; http://lattes.cnpq.br/3882851981484245
Among the various species of medicinal plants, we can find the Spilanthes acmella species, popularly known as Jambu that stands out due to its numerous applications in the folk medicine field. Traditional medicine recommends the use of its leaves and flowers in the preparation of infusions to treat anemia, dyspepsia, malaria, mouth diseases (tooth pain) and throat diseases, against scurvy and also as antibiotic and anesthetic, being its main effects attributed to espilantol, which is an important representative of the substances present in these plants. Some studies have been performed using the espilantol, providing some information of the effect of this substance, as its immunomodulatory effect and because of its functional interaction with monocytes, granulocytes and killer cells. However, there are still no electrophysiological studies about its ictiotoxic action using, for example, the EEG to demonstrate its action at the central nervous system level or electromyogram to verify the occurrence of their effects in the Zebrafish muscles, evoking the need for this research. Based on this, the present study aimed to investigate the ictiotoxic action of the ethanol extract of the Spilanthes acmella root in Zebrafish by electrophysiological and behavioral analysis. The results showed that the ethanol extract of Spilanthes acmella is a potent inducer of central excitability in zebrafish, this being evidenced by changes in electrical activity patterns seen in the EEG of the animals subjected to the extract and by increasing brain activity seen in the spectrogram. The extract also caused changes, in a lesser extent, on the electromyographic tracings of zebrafish subjected to the same concentration of the extract, with the appearance of scattered muscle contractions and brief myoclonus. And the behavioral findings from the delimitation of three stages of behavior, which began with the increased excitability of the animal and resulted in the seizure and death of the fish, served to corroborate the electrophysiological findings that the ethanol extract of Spilanthesacmella acts as a potent substance acting on the nervous system of zebrafish, with convulsant activity.
Acesso aberto (Open Access)
Obtenção de um produto desidratado à base de tucupi, jambu e banana verde através do processo de Refractance Window
(Universidade Federal do Pará, 2014-11-10) SANTOS, Paula Hellayne Costa dos; SILVA, Luiza Helena Meller da; http://lattes.cnpq.br/2311121099883170
Given the immense diversity of fruits, vegetables and products developed in northern Brazil, tucupi, jambu and banana are notable because they are widely used, not only in culinary of Pará, but for several other purposes. Aiming to use these abundant products in the northern Brazil, the present work aimed at obtaining a dehydrated product based on tucupi, jambu and green banana, through the Refractance Window process. The formulations were dried at a temperature of 70 ° C, in a time of 50 min. Two formulations coded as FORM I and FORM II were dried. The initial moisture content of the formulations was 88% wb, and the values of final moisture content of the dehydrated product were 10% (for FORM I) and 9% (for FORM II). The drying time of the samples varied from one formulation to another, and the time was shorter for the drying of FORM II. The dried products had high water absorption capacity (2159.091 for FORM II) and (1666.667 for FORM I) and had an average oil absorption capacity. As to hygroscopicity, the products were considered little hygroscopic: 7.9 and 9.27 g H2O / g of sample for FORM I and FORM II, respectively. The dehydrated products showed significant content of phenols: 221.18 and 169.49 mg GAE / 100 g of sample for FORM I and FORM II, respectively. Regarding antioxidant capacity, the dehydrated products had higher antioxidant capacity by DPPH radical sequestration than the ABTS radical. For the results of color, the values (ΔE *) varied significantly for the dehydrated products with the same drying conditions. The shelf life was evaluated for 35 days and it was observed that the dried product of the Form I showed no change from seventh day, when evaluated by moisture gain, whereas FORM II showed variation during the entire period of storage . When the assessed parameter was the water activity, the FORM II did not change from the 14th day, while the FORM I changed only in twenty-first day of storage.
Acesso aberto (Open Access)
Utilização de técnicas hifenadas para o desenvolvimento de programações para analise de Acmella oleracea (L.) RK Jansen
(Universidade Federal do Pará, 2016-08-31) SILVA, Ianna Dias Ribeiro da; REBELO, Monaliza Maia; http://lattes.cnpq.br/2064401199679916; BARBOSA, Wagner Luiz Ramos; http://lattes.cnpq.br/1372405563294070
The use of hyphenated techniques that allow the separation and isolation of substances of a herbal extracts is necessary both for the knowledge of the chemical composition of a compound, as well as for the determination of a substance or a group that serve as marker of particular species. The species Acmella oleracea (l.) R. K. Jansen, grows in Legal Amazon, a geographic region in northern Brazil, where is known as Jambu, Agrião Bravo ou Agrião do Pará. It is used in folk medicine in the form of tea, syrups and tinctures and indicated for use as anesthetic and antiseptic. The aim of this study was to use the HPLC-UV-ELSD and HPLC-DAD-MS for the development of methods that can identify metabolites of interest on Acmella oleracea. The plant material was acquired in Tailândia, State of Pará, in the Santa Inês farm, city of Tomé-Açu, PA150 highway margins, positioned the 260 km from Belém, at 02° 56 ' 22 '' south latitude and 48° 57 ' 03 '' west longitude, and consisted of 10 packs of Acmella oleracea (jambu), composed of leaves, flower, root and stem, after cleaning the material was grinded and then macerated for 7 days with 92.8° GL ethanol at a ratio of 1:10. Hydroethanolic extracts, of each part of the plant, were filtered and concentrated at a low pressure and then lyophilized for further analysis with hyphenated techniques. The Spilanthol was registered in the analysis by HPLC-DAD-ESI-MS, Scan mode and Product ion, through the detection of ion m/z 222 in all analyzed organs. A substance which showed m/z 376.80 for analysis with etoh/water and m/z 388.20 for acn/water, which has promising profile was found through the analysis by HPLC-DAD-MS and needs specific experiments for confirmation of flavonoids. During the development of methods, it was observed that the best chromatographic profiles of EEAOFOC and EEAOR were obtained when the mobile phase was composed of ethanol and water.