Navegando por Assunto "Medicina tropical"

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Acesso aberto (Open Access)
Analysis of polymorphisms in the interleukin 18 gene promotor (-137 g/c and -607 c/a) in patients infected with hepatitis c virus from the brazilian amazon
(Universidade Federal do Pará, 2015-09) SANTOS, Kemper Nunes dos; ALMEIDA, Marcella Kelly Costa de; FECURY, Amanda Alves; COSTA, Carlos Araújo da; MARTINS, Luisa Caricio
Background - The hepatitis C virus has been recognized as the leading cause of chronic liver disease in the world. Host genetic factors have been implicated in the persistence of hepatitis C virus infection. Single nucleotide polymorphisms at positions -607 C/A (rs1946518) and -137 G/C (rs187238) in the IL-18 gene promoter have been suggested to be associated with delayed hepatitis C virus clearance and persistence of the disease. Objective - Identify these polymorphisms in a population infected with hepatitis C virus from the Brazilian Amazon region. Methods - In a cross-sectional analytical study conducted in Belém, Pará, Brazil, 304 patients infected with hepatitis C virus were divided into two groups: group A, patients with persistent infection; group B, patients with spontaneous clearance. The control group consisted of 376 volunteers not infected with hepatitis C virus. Samples were analyzed by RT-PCR for the detection of viral RNA and by RFLP-PCR to evaluate the presence of the -137 G/C and -607 C/A IL-18 gene promoter polymorphisms. Results - Comparison of polymorphism allele frequencies between the patient and control groups showed a higher frequency of allele C at position -607 among patients (P=0.02). When the association between the polymorphisms and viral infection was analyzed, patients carrying genotype C/A at position -607 were found to be at higher risk of persistent hepatitis C virus infection (P=0.03). Conclusion - the present results suggest a possible role of the -607 IL-18 gene promoter polymorphism in the pathogenesis of hepatitis C virus infection.
Acesso aberto (Open Access)
Caracterização dos genótipos do vírus da hepatite b em pacientes atendidos no programa de hepatites virais do núcleo de medicina tropical – UFPA, Belém - Pará
(Universidade Federal do Pará, 2016-05-27) ALMEIDA, Marcella Kelly Costa de; MARTINS, Luisa Caricio; http://lattes.cnpq.br/1799493244439769
The hepatitis B virus belongs to the genus Orthohepdnavirus and Hepadnaviridae family, comprising a DNA virus capable of infecting mammalian hepatotropic. Classified into 10 genotypes (A-J) and many different subgenotypes, studies suggest that they may influence the severity of the disease, response to treatment and vaccine response. Genotypes and subgenotypes HBV has a wide distribution, some being restricted to certain geographic regions, while others show a worldwide distribution. It is found in different regions of Brazil with prevalence of genotypes A, D and F. This study aimed to identify genotypes and subgenotypes of hepatitis B virus among patients treated at the Tropical Medicine Center - UFPA, in the city of Belém, state Pará, from January 2011 to December 2015. A total of 1274 patients treated at the NMT-UFPA in the period, were selected for the study 222 patients of both sexes, with positive serology for HBsAg and / or anti-HBc. The samples were subjected to molecular biology, PCR "in house" and multiplex PCR to detect viral DNA and genotyping, and then the sequencing for confirmation and determination of viral subgenotypes. In 65 of the 222 samples was detected the presence of HBV DNA, but only 63 samples were satisfactory after the result of sequencing, which were identified the presence of genotypes A (46/63), especially the subgenotype A1 (36/46) followed by subgenotype A2 (10/46), and genotype F (17/63) and only detected subgenotype F2 (17/17) circulating in this population. The average age of the patients was 38 years, predominantly male, and most natural state of Pará patients. Some risk factors have been identified among the population studied, and not using a condom during sex the most predominant. Comparing the presence of viral DNA with serology for HBsAg marker is shown a picture that suggests the presence of occult hepatitis among the patients treated. The results found in this study are consistent with what is reported in other studies in Brazil.
Acesso aberto (Open Access)
As expedições da Liverpool School of Tropical Medicine e a Amazônia Brasileira
(2011-06) MIRANDA, Aristóteles Guilliod de; ABREU JUNIOR, José Maria de Castro
This paper describe the early years of the Liverpool School of Tropical Medicine, and the expeditions to Brasilian Amazon, to the Cities of Belém and Manaus describing also what happened with the reserchers involved Herbert Durhan, Walter Myers and Harold Wolferstan Thomas.
Acesso aberto (Open Access)
Isolation of Fonsecaea pedrosoi from thorns of Mimosa pudica, a probable natural source of chromoblastomycosis
(2004-02) SALGADO, Claudio Guedes; SILVA, Jorge Pereira da; DINIZ JUNIOR, José Antônio Picanço; SILVA, Moisés Batista da; COSTA, Patricia Fagundes da; TEIXEIRA, Cláudio Eduardo Corrêa; SALGADO, Ubirajara Imbiriba
We report the isolation of Fonsecaea pedrosoi from thorns of the plant Mimosa pudica L. at the place of infection identified by one of our patients. Clinical diagnosis of chromoblastomycosis was established by direct microscopic examination and cultures from the patient's lesion. The same species was isolated from the patient and from the plant. Scanning electron microscopy of the surface of the thorns showed the characteristic conidial arrangement of F. pedrosoi. These data indicate that M. pudica could be a natural source of infection for the fungus F. pedrosoi.