Navegando por Assunto "Medicina veterinária"

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Acesso aberto (Open Access)
Cloning, expression and characterization of SeM protein of Streptococcus equi subsp. equi and evaluation of its use as antigen in an indirect ELISA
(2014-08) MORAES, Carina Martins de; CONCEIÇÃO, Fabrício Rochedo; ROCHA, Andréa da Silva Ramos; SANTOS JÚNIOR, Alceu Gonçalves dos; RIBAS, Leandro dos Montes; VARGAS, Agueda Palmira Castagna de; NOGUEIRA, Carlos Eduardo Wayne; TURNES, Carlos Gil; LEITE, Fabio Pereira Leivas
Strangles is an economically important horse disease caused by Streptococcus equi subsp. equi. The diagnosis can be confirmed either directly by bacterial isolation and PCR or by ELISA, which is an indirect method based on the detection of serum antibodies. The aim of this study was to clone, express and characterize the SeM protein of Streptococcus equi subsp. equi, evaluate its use as antigen in indirect ELISA and determine its performance to distinguish sera of negative, vaccinated and positive animals. This was initially performed by cloning the gene encoding the SeM protein and its expression in Escherichia coli. Subsequently, the protein produced was characterized and used as antigen in ELISA. Serum samples for evaluation were taken from 40 negative foals, 46 horses vaccinated with a commercial vaccine against strangles and 46 horses diagnosed with the disease. The test showed high specificity and sensitivity, allowing discrimination between negative and positive, positive and vaccinated animals, and vaccinated animals and negative sera. Thus, it was concluded that the protein produced rSeM, which can be used as antigen for disease diagnosis, and the described ELISA might be helpful to evaluate the immune status of the herd.
Acesso aberto (Open Access)
Determinação da ocorrência de anticorpos anti-Leptospira spp. em búfalos (Bubalus bubalis) criados no bioma Amazônico Paraense
(Universidade Federal do Pará, 2015-12-04) LEAL, Mario Arthur da Costa; SALVARANI, Felipe Masiero; http://lattes.cnpq.br/3339195791140438
This study investigated the occurrence of Leptospira spp. in buffaloes in the Amazon biome of Pará, as well as identify the higher incidence of serotypes in the region. They tested 387 serum samples of buffalo nine Pará municipalities, Cachoeira do Arari, Santa Cruz do Arari, Salvaterra, Soure and Chaves located on Marajó Island, Abaetetuba, Ipixuna, Nova Timboteua and Paragominas, located on the mainland. Eight serotypes were tested: Bataviae, Bratislava, Hardjobovis, Hardjo (OMS), Hardjo (CTG), Icterohaemorrhagiae, Pomona and Hardjo (Bolivia), through the microscopic agglutination test method (SAM). Of the total samples tested 354 (91.50%) were reactive serum for at least one serovar and 33 (8.50%) were negative. Three serotypes of serogroup Sejroe were more frequent, Hardjo (Bolivia) (79.30%), Hardjo (OMS) (64.8%) and Hardjobovis (64.10%). It is important to know the higher incidence of serotypes in the region studied so that the directions of combat and infection control are efficient, minimizing the potential risk of contamination and maintenance of leptospirosis in the herd.