Navegando por Assunto "Medula espinhal – Ferimentos e lesões"

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Acesso aberto (Open Access)
Efeitos anti-inflamatórios e neuroprotetores do extrato de cipó-pucá (Cissus verticilata) após lesão aguda da medula espinhal de ratos adultos
(Universidade Federal do Pará, 2019-02-15) LIMA, Kelly Correa; FRANCO, Edna Cristina Santos; http://lattes.cnpq.br/5939607544965550; LEAL, Walace Gomes; http://lattes.cnpq.br/2085871005197072
Acute spinal cord injury (aSCI) is a serious pathological condition that affects several individuals in different regions of the world and may cause physical and/or psychological sequelae. The available treatment is ineffective, which demands on development of new therapeutic approaches. The development of neuroprotective agents is of fundamental importance for the tissue preservation after aSCI. In the Amazon rain forests there are a multitude of medicinal plants, whose potential anti-inflammatory and neuroprotective effects have not been investigated. Cipó-pucá (Cissus verticillata) is used for treatment of stroke in folk medicine, but its effects are not scientifically proven and have not been investigated after aSCI. In this study, we investigated the anti-inflammatory and neuroprotective effects of the supercritical extract of cipó-pucá in an experimental model of aSCI in adult rats. The extracts of leaves of cipó-pucá were obtained by extraction with supercritical fluid. The animals underwent partial hemisection surgery of the spinal cord and were treated with extract of cipó-pucá (50 mg / kg) or vehicle. They were perfused at 24 hours postinjury. The gross histopathology was performed by hematoxylin-eosin staining. Immunohistochemical analysis for visualization of neurons, microglia, astrocytes and neutrophils were performed using antibodies against NeuN, CD68, caspase-3 and MBS-1, respectively. The quantitative analysis showed neuronal preservation and, reduction of the apoptotic cells, activated microglia and inflammatory infiltrate (neutrophils) in treated animals compared to the control group, suggesting a neuroprotective and anti-inflammatory effect of the supercritical extract of cipó-pucá in the aSCI model.
Acesso aberto (Open Access)
Ruptura do tendão calcâneo induz alterações bioquímicas e histológicas na medula espinhal de camundongos
(Universidade Federal do Pará, 2019-07-08) FRANÇA, Martha de Souza; SILVA, Anderson Manoel Herculano Oliveira da; http://lattes.cnpq.br/8407177208423247
The pathophysiology of the tendons involves release of neuronal mediators that play an active role in regulating tendon pain, inflammation and homeostasis. New directions have pointed out that injury is not restricted to tissue structural changes but indicates a possible involvement of the CNS in the regulation of the lesion. In this way, it is still unknown if the tendon injury affects the CNS, so the present study aims to investigate possible histological and biochemical changes in the spinal cord (L5) caused by the total rupture of the Achilles tendon in murine model. For this, the animals were submitted to tenotomy of the Achilles tendon, and separated in three groups (n = 36): Control; Rupture and Rupture+Suture. The total number cells of the spinal cord gray matter in the L5 vertebral segment was assessed by DAPI labeling. Glial reactivity was assessed by immunohistochemistry for microglia (IBA-1) and astrocytes (GFAP) at 7, 14 and 21 days after tendon rupture. The participation of the nitrergic system was investigated by the quantification of tissue levels of nitrite in lumbar intumescence at 7, 14 and 21 days post-injury and by iNOS (NOS2) immunostaining in L5. Statistical analyzes were performed using the ANOVA-1way test and post-test tukey, considering a significant p <0.05. The results were expressed as mean ± SD. The analysis of the number of cells showed that the Rupture group had a lower number of cells in 7 (1408.33 ± 58.59, p <0.05), 14 (1402.7 ± 72.7, p <0.05) and 21 (1374.5 ± 74.2, p <0.01) days post-injury in relation to the Control group (1668 ± 52.3) and in relation to the Rupture + Suture group on days 7 (1655 ± 66.5 , p <0.05) and 21 (1668.3 ± 14.1, p <0.01). The Suture group did not differ from the Control group. The results of glial reactivity showed that at 14 days after injury the microglia were activated at L5 and that astrocytes were activated at 7, 14 and 21 days after injury. The nitrite quantification showed higher levels of nitrite in the group Rupture in 7 (0.0004 ± 10.8x10-5, p <0.01) and 14 days (0.0006 ± 1.06 x10-5, p <0.01) post-injury in relation to the control group (0.0002 ± 3.45x10-5). Immunostaining for iNOS was identified at 14 days after injury in the Rupture group. Our results showed that the rupture of the Achilles tendon induces changes in the spinal cord in terms of total cell number, activation of glial cells and participation of the nitrergic system in a murine experimental model. In this way, it points to possible degenerative, oxidative, inflammatory and neural plasticity events in the spinal cord resulting from the Achilles tendon injury, highlighting the CNS participation in the repair process of this lesion.