Navegando por Assunto "Microorganismos"

Agora exibindo 1 - 4 de 4

Acesso aberto (Open Access)
Annularins I and J: New Metabolites Isolated from Endophytic Fungus Exserohilum rostratum
(Universidade Federal do Pará, 2016-08) PINHEIRO, Eduardo Antonio Abreu; BORGES, Fábio Cardoso; PINA, Jeferson Rodrigo Souza; FERREIRA, Leila do Remédio Santos; CORDEIRO, Jorgeffson da Silva; CARVALHO, Josiwander Miranda; FEITOSA, André de Oliveira; CAMPOS, Francinete Ramos; BARISON, Andersson; SOUZA, Afonso Duarte Leão de; MARINHO, Patrícia Santana Barbosa; MARINHO, Andrey Moacir do Rosário
The paper describes the isolation of ergosterol peroxide, monocerin, annularin I and annularin J compounds of Exserohilum rostratum biomass extracts. Compounds were isolated by silica gel column chromatography in polarity gradient and the structures were determined by nuclear magnetic resonance (NMR) and mass spectrometry (MS) methods. Monocerin, annularin I and annularin J compounds were tested against Escherichia coli, Pseudomonas aeruginosa and Bacillus subtilis bacteria and showed moderate activity. Annularin I and annularin J compounds are new natural products.
Acesso aberto (Open Access)
Potencial de produção de antimicrobianos a partir de bactérias isoladas do açaí (Euterpe oleracea)
(Universidade Federal do Pará, 2015-08-17) SALLES, Marina Ludmila Oliveira Conor; SANTOS, Agenor Valadares; http://lattes.cnpq.br/9530734927662735
Açaí is the fruit of a palm tree widely distributed in the Amazon region and has a high microbial load that favors the fruit fermentation process. It is unknown specific studies to investigate the production of antimicrobial substances of biotechnological interest from microorganisms present in acai fruit, which can result in increased value to the fruit. Since food safety has become an increasingly international concern the application of bacteriocins that target food pathogens without adverse toxic effects, have received great attention. The objective of this work is to isolate bacteria from acai, identify the antimicrobial activity of the isolated bacteria, and characterize the extracts of these bacteria as pH, temperature, stability and action spectrum. The selected fruits were collected in Combu Island and Abaetetuba, State of Para, Brazil. These followed for asepsis and pulp removal and dilutions were inoculated in MRS agar and isolated for analysis The antagonism in vitro was performed with the technique of spot-on-the lawn revealing against three bacteria: Corynebacterium sp, Listeria monocytogenes and Bacillus cereus; and with the well diffusion technique. These extracts were characterized for thermal stability (121 ° C / 15 minutes), enzyme sensitivity to trypsin and pH neutralization. Of the thirty-nine bacterial isolates, it was observed antagonistic activity in nineteen of them. Of thesecould be observed twelve showed extracellular antagonist activity. Four extracts showed sensitivity to trypsin, indicating that these may be bacteriocins. Neutralized extracts showed no activity, which indicates better activity at pH acid extracts. Most of the extracts showed activity after being autoclaved, which may be an indication that the extracts have bacteriocins in Classes I and II. This work shows that bacteria isolated from acai have potential for the production of antimicrobial substances.
Acesso aberto (Open Access)
Variabilidade quantitativa de população microbiana associada às condições microclimáticas observadas em solo de floresta tropical úmida
(2011-12) RODRIGUES, Hernani José Brazão; SÁ, Leonardo Deane de Abreu; RUIVO, Maria de Lourdes Pinheiro; COSTA, Antonio Carlos Lôla da; SILVA, Rommel Benicio Costa da; MOURA, Quêzia Leandro de; MELO, Ivan Fiuza de
This study is a proposal for a scientific contribution to the understanding of the interrelationships between density population of soil microorganisms associated with seasonal microclimatic variability in tropical rainforest, also considering a case study of extreme event. Some organisms, especially soil microorganisms are very sensitive to small variations in microclimate (sun light, temperature, soil moisture, wind, sensible heat, latent heat, etc). Surely these conditioning factors are important for understanding the spatial distribution of these living things in natural ecosystems, inhabited by a variety of microorganisms (mushroom and bacteria) that were studied for their distribution and density, using the technique of "Pour Plate" count of "Petri plates" following the methodology utilized for De-Polli and Guerra, described by Clark. This study was conducted in two experimental areas PPBio (natural rainforest area) and ESECAFLOR (one acre covered area to simulate drought) in Caxiuanã-PA, with continuous measurements of microclimatic variables such as temperature, humidity and rainfall, and the evaluation of spatial and temporal distribution patterns of the species abundance and richness, to establish a monitoring procedure of soil fungi and bacteria associated with climate variability in the national forest Caxiuanã. The experimental areas are predominantly Yellow Latossol soils. Microbiological analysis showed that fungi developed better during the dry season and bacteria during rainy season, and their populations decrease with depth, except in a changed environment. Correlation values between seasonal variation of fungi and bacteria population and varying temperature and soil humidity were satisfactorily for any season in both sites.
Acesso aberto (Open Access)
Variação sazonal da população de bactérias e fungos e dos teores de nitrato e amônio do solo nos sítios do LBA e PPBIO, na amazônia oriental
(Universidade Federal do Pará, 2015-09) MOURA, Quêzia Leandro de; RUIVO, Maria de Lourdes Pinheiro; RODRIGUES, Hernani José Brazão; ROCHA, Edson José Paulino da; SILVA JUNIOR, João de Athaydes; VASCONCELOS, Steel Silva; ANDRADE, Mariseth Carvalho de; MANES, Carmem Lara de Oliveira
It is possible that the environmental factors determining the behavior of soil microbiota are being modified by anthropic or natural climate change. In order to check the effect of the exclusion of water over the population of bacteria and fungi in the soil, the present study was developed. The chosen location is the area of the ESECAFLOR experiment, which simulates the occurrence of extreme phenomena such as El Niño event, and the area of biodiversity research program Program-PPBio (primary forest). This later area, supposed to be used for studying the Biodiversity of the Amazon, was used as a control area for comparative purposes. The soil was sampled at 00 - 05, 05 -10, 10-20 and 20-30 cm depths, during the rainy, transition and less rainy seasons. The highest values of Colonies Forming Units (CFU) for bacteria and fungi populations were 196 x 104 CFU/g of soil and 124 x 102 CFU/g of solo, respectively, both in the area without human intervention (PPBio). Soil moisture is the variable influencing most the obtained counting values of fungi and bacteria populations.