Navegando por Assunto "Morfina"

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Acesso aberto (Open Access)
Nocicepção em prole adulta de camundongo em exposição à morfina no período gestacional e lactação
(Universidade Federal do Pará, 2014-10-18) CASTRO, Nair Correia de Freitas; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
The use of morphine as a drug of abuse during pregnancy and lactation induces effects on the developing fetus are still not well elucidated, in which the perinatal exposure to morphine showed increased sensitivity to morphine reinforcement effect in the adult offspring. The present study investigated whether exposure to morphine during pregnancy and lactation can change nociception in adult offspring offspring of mothers treated with morphine. Pregnant female mice were exposed to morphine (10 mg / kg / day) subcutaneously for 42 days (21 days of pregnancy to 21 days of lactation). Upon completion 21 days, the progeny is sexed in males and females, then at 75 days of age, subjected to the open field test and nociception by the methods of writhing induced by acetic acid, hot plate and formalin. In the test of the total locomotion, the animals showed no motor changes. In nociception tests, we observed increased nociceptive response in male mice and female morphine group tested for contortion. Males who have been exposed perinatally to morphine decreased the nociceptive threshold in the second phase (inflammatory phase) of formalin. In the hot plate, the male and female animals showed abnormalities in pain sensitivity, reversing the sensitivity profiles of its controls, in which the group of males exposed perinatally to morphine showed increased sensitivity to thermal stimulus at 120 min test and the group of females exposed perinatally to morphine decreased thermal sensitivity when compared to their controls. These results suggest that exposure to morphine in the intrauterine period and lactation affects nociceptive thresholds in the offspring in later life and that this change is dependent on the type and length of exposure to noxious stimuli.
Acesso aberto (Open Access)
Therapeutic concentration of morphine reduces oxidative stress in glioma cell line
(2014-05) ALMEIDA, Mauro Brito de; MALAQUIAS, Allan Costa; NASCIMENTO, José Luiz Martins do; OLIVEIRA, Karen Renata Matos; SILVA, Anderson Manoel Herculano Oliveira da; CRESPO LÓPEZ, Maria Elena
Morphine is a potent analgesic opioid used extensively for pain treatment. During the last decade, global consumption grew more than 4-fold. However, molecular mechanisms elicited by morphine are not totally understood. Thus, a growing literature indicates that there are additional actions to the analgesic effect. Previous studies about morphine and oxidative stress are controversial and used concentrations outside the range of clinical practice. Therefore, in this study, we hypothesized that a therapeutic concentration of morphine (1 μM) would show a protective effect in a traditional model of oxidative stress. We exposed the C6 glioma cell line to hydrogen peroxide (H2O2) and/or morphine for 24 h and evaluated cell viability, lipid peroxidation, and levels of sulfhydryl groups (an indicator of the redox state of the cell). Morphine did not prevent the decrease in cell viability provoked by H2O2) but partially prevented lipid peroxidation caused by 0.0025% H2O2) (a concentration allowing more than 90% cell viability). Interestingly, this opioid did not alter the increased levels of sulfhydryl groups produced by exposure to 0.0025% H2O2), opening the possibility that alternative molecular mechanisms (a direct scavenging activity or the inhibition of NAPDH oxidase) may explain the protective effect registered in the lipid peroxidation assay. Our results demonstrate, for the first time, that morphine in usual analgesic doses may contribute to minimizing oxidative stress in cells of glial origin. This study supports the importance of employing concentrations similar to those used in clinical practice for a better approximation between experimental models and the clinical setting.
Acesso aberto (Open Access)
Uso da morfina no período gestacional e lactação: efeitos sobre o desenvolvimento do sistema nervoso central e comportamento
(Universidade Federal do Pará, 2013-09-17) SOUZA, Izabelle Camões de; MAIA, Cristiane do Socorro Ferraz; http://lattes.cnpq.br/4835820645258101
The use of psychoactive substances is a major problem in modern society, causing social upheaval, economic and health of the user. In 2010, 26,4-36 million people used opioid analgesics for non-therapeutic purposes, and the frequency of alcohol abuse by health professionals, five times greater than the general population, due to easier access. The prevalence in use during pregnancy introduces rates ranging from 1% to 21%, accounting concern in society, since the effects caused on fetal development are not well understood. The aim of this study was to evaluate the neurobehavioral responses of adult rats after chronic exposure to morphine during the intrauterine period and lactation. Pregnant rats were given for 42 days subcutaneously, morphine 10 mg / kg / day. The offspring were weighed on days D1, D5, D10, D15, D20, D30, D60, and behavioral assays performed with the progeny to 2.5 months of age, which consisted of the open field model, elevated plus maze, forced swimming and rota-rod. The results demonstrated that exposure to morphine promote changes in the weight gain after the birth of offspring and even after weaning increase in spontaneous locomotion in females, as well as increased anxiogenic-like behavior and the behavior of the depressive type, regardless of sex, but without prejudice associated motor.