Navegando por Assunto "Mycobacterium tuberculosis"

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Acesso aberto (Open Access)
CMRegNet–An interspecies reference database for corynebacterial and mycobacterial regulatory networks
(BioMed Central Ltd, 2015-06) ABREU, Vinicius Augusto Carvalho de; ALMEIDA, Sintia Silva de; TIWARI, Sandeep; HASSAN, Syed Shah; MARIANO, Diego César Batista; SILVA, Artur Luiz da Costa da; BAUMBACH, Jan; AZEVEDO, Vasco Ariston de Carvalho; RÖTTGER, Richard
Background: Organisms utilize a multitude of mechanisms for responding to changing environmental conditions, maintaining their functional homeostasis and to overcome stress situations. One of the most important mechanisms is transcriptional gene regulation. In-depth study of the transcriptional gene regulatory network can lead to various practical applications, creating a greater understanding of how organisms control their cellular behavior. Description: In this work, we present a new database, CMRegNet for the gene regulatory networks of Corynebacterium glutamicum ATCC 13032 and Mycobacterium tuberculosis H37Rv. We furthermore transferred the known networks of these model organisms to 18 other non-model but phylogenetically close species (target organisms) of the CMNR group. In comparison to other network transfers, for the first time we utilized two model organisms resulting into a more diverse and complete network of the target organisms. Conclusion: CMRegNet provides easy access to a total of 3,103 known regulations in C. glutamicum ATCC 13032 and M. tuberculosis H37Rv and to 38,940 evolutionary conserved interactions for 18 non-model species of the CMNR group. This makes CMRegNet to date the most comprehensive database of regulatory interactions of CMNR bacteria. The content of CMRegNet is publicly available online via a web interface found at http://lgcm.icb.ufmg.br/cmregnet.
Acesso aberto (Open Access)
Investigação de polimorfismos no gene TNF em pacientes com hepatotoxicidade induzida por medicações antituberculosas no norte do Brasil
(Universidade Federal do Pará, 2015-08-27) VALENTE, Sonia Lopes; SANTOS, Ney Pereira Carneiro dos; http://lattes.cnpq.br/1290427033107137; SORTICA, Vinicius de Albuquerque; http://lattes.cnpq.br/2046482071071824
Tuberculosis still remains a serious public health problem worldwide. The hepatotoxicity induced by anti-tuberculosis drugs causes a large number of hospitalizations and may be fatal if treatment is not interrupted. The hepatitis induced by anti-tuberculosis drugs are not yet fully understood and clinical studies suggests that immunological mechanisms are involved in its pathogenesis. The cytokine TNF-α is a major mediator of inflammatory and immune changes in the levels of this cytokine may be related to pathogenesis of drug-induced hepatitis. These changes observed may be related to polymorphisms in the TNF gene. The knowledge of which polymorphisms in the TNF gene are involved in the risk of developing hepatotoxicity anti-tuberculosis drugs will permit the use of these molecular markers to improve the therapeutic management of these patients. This study investigated the influence of polymorphisms -308C>T (rs1800629), -1031C>T (rs1799964), -238A>G (rs361525) and -857C>T (rs1799724) in the TNF gene with drug-induced hepatotoxicity. The study included 68 patients with tuberculosis who had hepatotoxicity of the basic regimen consisting of rifampicin, isoniazid, pyrazinamide and ethambutol (2RHZE/4R) and 191 patients without adverse therapy effects. The polymorphisms were determined by real-time PCR with TaqMan probes. Comparing the frequency of genotypes between cases and controls, a significant difference in the distribution of genotypes of the SNP -1031C>T was identified (p = 0.003). The frequency of homozygous -1031CC was higher in the case group (8.8%) than in the control group (1.6%). The -1031CC homozygous patients had an increased risk for the development of hepatotoxicity when compared to homozygous -1031TT or the T allele carriers (OR = 8.632, p = 0.014, OR = 11.355, p = 0.004). We concluded that -1031C>T SNP was significantly associated with susceptibility to induced hepatitis anti-tuberculosis drugs in the north population of Brazil.
Acesso aberto (Open Access)
Investigação de polimorfismos nos genes IFNɣ e INFGR1 associados à tuberculose no estado do Pará
(Universidade Federal do Pará, 2015-11-06) CARNEIRO, Klezzer de Oliveira; SANTOS, Ney Pereira Carneiro dos; http://lattes.cnpq.br/1290427033107137; ASSUMPÇÃO, Paulo Pimentel de; http://lattes.cnpq.br/7323606327039876
Pulmonary tuberculosis is an infectious disease transmission by air, which according to the World Health Organization (WHO), infects about two billion people around the world. It is the leading cause of death from infectious disease in adults in developing countries, representing a serious public health problem, mainly due to non-adherence to treatment, late diagnosis and underdiagnosis and no control contacts, which makes our population susceptible to infection. The present study aimed to investigate associations three genetic polymorphisms in IFNɣ and INFGR1 genes responsible for susceptibility to tuberculosis in patients affected by the disease; evaluate if there are differences in allelic and genotypic frequencies of polymorphisms in genes IFNɣ 871A> T, INFGR1 611 (C> T) and INFGR1 -56 (A> G) among individuals with TB and those without TB population of Bethlehem. The control substructures effect was carried out by the use ofa 48 markers Informational Genetic Ancestry panel in both patient sample and the control sample. For this study we used peripheral blood samples from 148 patients diagnosed with tuberculosis, and 125 individuals without tuberculosis (controls) resident in the State of Pará, Brazil, attended at University Hospital João de Barros Barreto (HUJBB) during the period from 2006 to 2012. each specimen was obtained 5 ml of venous blood collected from a peripheral vein. DNA extraction was performed according to the method described by Sambrook et al., (1989). Genotyping for polymorphisms IFNɣ gene (rs1130562) and INFGR1 (rs1327474, rs2234711) was performed by PCR in real time (RTQ-PCR) using the TaqMan system. Statistical analyzes were performed in SPSS 17.0 software, using the Mann- Whitney test, with significance set at p <0.05. The results did not show significance of the polymorphisms investigated in relation to susceptibility to tuberculosis.
Acesso aberto (Open Access)
Nested-PCR do gene que codifica o antígeno b aplicada ao diagnóstico da tuberculose pulmonar
(2007-04) LIMA, Karla Valéria Batista; LOPES, Maria Luíza; LOUREIRO, Edvaldo Carlos Brito; COSTA, Maurimélia Mesquita da; CARDOSO, Ninarosa Calzavara; LIMA, George Leandro Ferreira; SOUSA, Maísa Silva de
The polymerase chain reaction used for amplifying an internal sequence of a previously amplified fragment (nested-PCR) was investigated as a complementary alternative for searching for alcohol-acid resistant bacilli and Mycobacterium tuberculosis cultures in Lowenstein-Jensen medium. 144 sputum samples were investigated from patients with suspected tuberculosis that were sent to the Tuberculosis Laboratory of the Evandro Chagas Institute in Belém, between June 2002 and December 2003. From the 144 samples, 121 were characterized as tuberculosis: 119 were positive in cultures, 95 under bacilloscopy and 128 using nested-PCR. The sensibility of the nested-PCR was 96% (116/121), while the specificity was 48% (11/23). Nested-PCR may be a complementary tool for diagnosing tuberculosis, since it presents sensitivity equivalent to that of cultures. However, further evaluations are needed with the aim of minimizing the number of false-positive results.
Acesso aberto (Open Access)
Uso do spoligotyping para genotipagem de Mycobacterium tuberculosis obtidos a partir de lâminas coradas pela técnica de ziehl-neelsen pertencentes a pacientes com tuberculose pulmonar residentes nos municípios paraenses de Belém e Ananindeua, Pará, Brasil
(Universidade Federal do Pará, 2010-06-10) FURLANETO, Ismari Perini; LIMA, Karla Valéria Batista; http://lattes.cnpq.br/9795461154139260
Tuberculosis (TB) is a major public health problem, closely linked to socioeconomic factors, and its main causative agent Mycobacterium tuberculosis. The Spoligotyping is based on PCR-reverse hybridization that permit identification and differentiation of M. tuberculosis Complex members directly from clinical samples, as samples from Ziehl-Neelsen stained slides (ZN), avoiding problems associated with slow growth of these microorganisms, thus becoming an important tool for the monitoring of strains in different epidemiological settings, being able to reveal the biogeographical character of these. The ability to characterize genetic, demographic and geographically these microorganisms can contribute both to understanding how the disease is transmitted and the implementation of actions to its control and combat. Thus, we performed a retrospective study that evaluated the samples obtained from ZN stained slides, made by public heath laboratories from Belém and Ananindeua between October 2007 and March 2008. Most (61.3%) of 163 cases included in the study belonged to the males and 68.0% of cases were between 20 and 49 years with a mean age of 38 years. Application of Spoligotyping in this kind of samples showed good performance, with 146 (89.6%) hybridization patterns of complete and consistent with each other after the duplicates. Of these, 142 were considered for comparison with the international database of Spoligotypes (SpolDB4), of which 67 were observed different spoligotype or genotypes, including 95 (67%) cases were shared by two to 20 samples and 47 (33%) with unique patterns. Forty-eight (71.6%) genotypes were known and 19 (28.4%) have not been reported in SpolDB4. LAM and T families were more frequent, concentrating 56 (39.4%) and 35 (24.6%) cases, respectively, and Haarlem, and EAI families comprised 12 (8.45%) samples each. Spatial localization of cases allowed to visualize the distribution of spoligotypes in the cities studied, revealing some groups with the same genotype, proving to be useful to guide and assist future investigations.