Navegando por Assunto "Neoplasias pulmonares"

Agora exibindo 1 - 2 de 2

Acesso aberto (Open Access)
Análise de alterações moleculares nos genes ND1 e ND3 em câncer de pulmão não pequenas células na população paraense
(Universidade Federal do Pará, 2018-03-09) FERNANDES, Lorena Duarte; BORGES, Bárbara do Nascimento; http://lattes.cnpq.br/0676220027193876
Bronchopulmonary carcinoma is the most frequent in the world, being one of the most aggressive neoplasms, with a mortality / incidence ratio of around 90%, with overall survival in five years low, about 10 to 15%, in most populations of the world. In the Northern Region of Brazil, this pathology is the third most frequent among men and the fourth among women. From the anatomopathological point of view, lung cancer is classified into two main types: small cells and non-small cells, the latter being the most incident, accounting for 75% of cases. Currently, the distinction between subtypes is based on histological, immunohistochemical, and molecular differences. In this context, it is important to emphasize that molecular information influences not only diagnosis, prognosis, but also therapeutic behavior. Several genetic and epigenetic alterations of the nuclear genome are related to the pathogenesis of this tumor. However, changes in oxidative phosphorylation resulting from mitochondrial dysfunction have long been suggested as involved in the process of tumorigenesis. Thus, the present study analyzed two mitochondrial DNA (ND1 and ND3) genes belonging to the I complex of the mitochondrial respiratory chain in 66 lung tissue samples from patients with and without non-small cell lung cancer in the population of the state of Pará. the sequencing analysis identified four alterations in the ND1 gene: C3553T, T3552A, C3595ins and G3666A and only two changes in the ND3 gene: A10398G and C10400T. Among the alterations found in the ND1 gene, no statistical significance was observed in relation to the development of lung cancer. However, a structural alteration in the ND1 gene was found in the presence of C3595ins, not yet described in the literature. Whereas, the presence of the A allele, observed in T3552A in the ND1 gene, was significantly associated with a protective effect on the development of lung cancer. Already changes in the ND3 gene (G10398A and T10400C) were significantly associated with lung cancer, these changes in ND3 being potential for use as markers in patients with non-small cell lung cancer
Acesso aberto (Open Access)
Estudo de potenciais marcadores moleculares de suscetibilidade ao câncer de pulmão
(Universidade Federal do Pará, 2015-08-27) SILVA, Francisco Anderson; SORTICA, Vinicius de Albuquerque; http://lattes.cnpq.br/2046482071071824
Lung cancer is a major public health problem, currently occupying the tenth position among the leading causes of death worldwide and the leading cause of death among cancer. The individual predisposition to developing lung cancer could be associated with genetic polymorphisms related to the inflammatory response, activation mechanisms and detoxification or carcinogens, as well as defects in the mechanisms of the DNA repair. This study aimed to investigate the influence of 13 polymorphisms of the type insertion / deletion in genes of the metabolism and biotransformation (CYP2E1, CYP19A1 and UGT1A1), control genes of the immune system and inflammatory response (IL1A and IL4), genes that regulate control of gene function of the cell cycle and immune system (MDM2 and NFKB1), DNA repair genes (TYMS and XRCC1), regulator of apoptosis gene (CASP 8), regulator of hemostasis gene (PAR1) and control gene cell cycle (TP53) as susceptibility to lung cancer. Polymorphisms were genotyped by a multiplex PCR reaction of patients with a confirmed diagnosis for lung cancer and individuals from the same population without the disease. The genetic ancestry of all individuals were estimated by a panel of ancestry informative markers. A logistic regression analysis controlling for age, gender and smoking was performed to determine the influence of polymorphisms in susceptibility to cancer. No statistically significant differences between the groups with cancer and without cancer were founded. Polymorphisms studied are not associated with susceptibility to lung cancer in the Pará population.