Navegando por Assunto "Nociception"

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Acesso aberto (Open Access)
Avaliação farmacológica da fração ácida do óleo de copaíba intercalada em hidróxidos duplos lamelares
(Universidade Federal do Pará, 2017-10-03) BARBOSA, Aline da Silva; QUEIROZ, Luana Melo Diogo de; http://lattes.cnpq.br/9775224837043003; https://orcid.org/ 0000-0001-5036-5639
Copaiba oil is traditionally used in the Amazon region of Brazil for pharmacological purposes as pain and inflammation. The acidic fraction of copaiba oil (FAOC) consists of diterpenes and, among them, excels copalic acid as major component, being endowed with anti inflammatory activity in vitro. In addition to the research for new active principles, there is also interest in management models that optimize the action of drugs. For this, an alternative is the controlled release of drugs, which can be obtained by the intercalation of these in Lamellar Double Hydroxides (HDLs). Thus, this study aimed to evaluate the antinociceptive and anti-inflammatory activity of the FAOC and FAOC intercalates in HDL (FAOC-HDL) in vivo models of nociception and inflammation. Male (Mus musculus) mice (4 to 6 weeks old, weight 25-35g) were divided into groups (n = 6) and treated orally (0.1mL/10g), with negative control (vehicle), HDL, FAOC, FAOC-HDL, and positive control, 1h, 24h and 48h before each test. First, was determined the median effective dose (DE50) and then the experimental models of acetic acid-induced abdominal contraction, formalin test, croton oil induced ear edema, carrageenan-induced paw edema test and peritonitis induced by carrageenan. The ED 50 found was FAOC (98 mg/kg) and FAOC-HDL (222.9 mg/kg). After 1 h of treatment, FAOC (98 mg/kg) and FAOC-HDL (222.9 mg/kg) significantly inhibited the number of abdominal writhing respectively in 49.99% (22,67± 1,56) and 56,99% (19,5 ± 2,69), the paw licking time in the formalin test was 28.93% (113.6 ± 12.45) and 60.79% (62.67 ± 11, 02) in the second phase of the test, development of ear edema in 25.59% (2.53 ± 0.21) and 47.65% (1.78 ± 0.20) and development of paw edema in the 2nd and 3rd hour of the test and the migration of total leukocytes by 73.17% (1.95 ± 0.38) and 75.69% (1.77 ± 0.28) and neutrophils in 62.82 (1.61 ± 0.31), 91.0% (0.39 ± 0.10) in the peritonitis. There was also a significant effect on treatment with FAOC-HDL (222.9 mg/kg) after 24 h of treatment, inhibiting the number of abdominal writhes in 58.34% (20.0 ± 3.97), lambda time of the paw in the formalin test 33.97% (98.83 ± 13.64), the development of ear edema in 57.05% (2.47 ± 0.36) and the development of paw edema in the 3rd hour of the test in 30.28%, (0.27 ± 0.02). Thus, the results obtained show antinociceptive and anti-inflammatory effect of the FAOC and, underpinning this effect by the controlled release of FAOC intercalates in HDL.
Acesso aberto (Open Access)
Investigação da toxicidade oral aguda e propriedades farmacológicas de uma espécie do gênero Cassytha
(Universidade Federal do Pará, 2019-05-15) BARROS, Mayra Arouck; FONTES JÚNIOR, Enéas de Andrade; http://lattes.cnpq.br/7056265073849866; https://orcid.org/ 0000-0002-6186-9581
Plants have been present in human culture since its inception, being used for several purposes, including for the treatment of diseases. This practice has been handed down from generation to generation. The evolution of knowledge, however, demands broader approaches on plant species with therapeutic potential, in order to ensure safety and validate its traditional use. Cassytha filiformis, a species of the genus Cassytha, is used in folk medicine to treat cancer, trypanosomiasis, kidney disease and gonorrhea. Among its proven activities, the antiplatelet, vascular relaxing, antioxidant, cytotoxic, antihypertensive, hepatoprotective, antiepileptic, diuretic and alpha-adrenergic receptor antagonist effects stand out. Among its secondary metabolites, alkaloids have been identified for therapeutic benefit. To date, however, there are no studies that support the safety of its therapeutic application or that explore possible anti-inflammatory properties as the basis for its therapeutic actions. Acute oral toxicity was assessed according to OECD 425. Starting from an administration of 2000 mg / kg (v.o) of extract in rats, the hippocratic signs of toxicity, spontaneous locomotor activity, weight gain, water and food consumption, as well as relative body weight and hematological patterns at the end of the period. The antinociceptive activity was evaluated in mice, using the acetic acid induced writhing test and the formalin test (CEUA nº 1050140817). The cutoff dose of the extract did not promote hipocratic signs of toxicity or death. There were also no changes in feed and water consumption or weight gain patterns. The evaluation of the relative weight of organs (liver, kidneys, stomach and heart) and hemogram showed equivalent standards between treated and control animals. Cas01 also did not impair the locomotor activity of the animals. Cas01 was shown to have no influence on the contortions induced by acetic acid, nor did it promote significant alterations on formalin-induced biphasic nociception. These findings demonstrate for the first time that Cas 01 is a xenobiotic of low acute oral toxicity. They also demonstrate that their therapeutic actions do not involve nociceptive or inflammatory mechanisms.