Navegando por Assunto "Ovinos"

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Acesso aberto (Open Access)
Avaliação de diferentes diluentes na criopreservação de sêmen ovino (Ovis aries)
(Universidade Federal do Pará, 2010-08-31) GUIMARÃES, Adrianne Araújo; VALE, William Gomes; http://lattes.cnpq.br/7486151987920142
During cryopreservation are numerous changes experienced by the sperm cells, which leads to the decrease in motility and loss of viability after thawing. For this reason, there arises the need to refine the process technology of semen, especially regarding the use of diluents. The aim of this study was to evaluate the viability of spermatozoa undergoing the process of cryopreservation and dilution, using three extenders (TES, TRIS and PBS) and three years of stability (4h, 8h and 12h), and the study was divided into nine groups: TES-4h-8h TES, TES- 12h, TRIS-4h-8h TRIS, TRIS-12h-4h PBS, PBS and PBS-8h-12h. After collection, semen was evaluated macro and microscopically, and pre-diluted solutions in TES, TRIS and PBS, but without the addition of cryoprotectants (Solution A), where they remained for 1 hour. Subsequently, semen was diluted with solutions TES, TRIS and PBS, already containing the cryoprotectants, again assessed to be so packed, and subjected to different periods of equilibrium, and then frozen. After thawing, motility was assessed and vigor, and after the TTR, motility, vigor and detachment of the acrosome. On cryopreservation with TRIS, motility and vigor were statistically similar (p> 0.05) in semen frozen with a balance of 4h (17.2% and 1.6), 8h (22.4% and 2.1) and 12h (14.8% and 1.6) (p> 0.05). After the TTR was not observed statistical difference (p> 0.05) in motility and vigor in 4h (10.4% and 1.1) and 12h (10.0% and 1.2) of balance, but there was an increase in 8h (15.6% and 1.6) (p <0.05). There was no statistical difference (p> 0.05) in the rate of detachment of the acrosome between 4h (35.1) and 12 (37.6) equilibrium, with a reduction in these indices at 8 am (30.4) (p <0 , 2005). On cryopreservation with ERT, the motility of frozen semen with a balance of 4h (24.8%) and 12h (27.6%) were statistically similar (p> 0.05), although the time of 8h (40.4% ) differed significantly from the others. The force was statistically similar (p> 0.05) for thawed-balanced 4h (2.0), 8h (2.6) and 12h (2.3), not statistically different (p> 0.05) . After the TTR was not observed statistical difference (p> 0.05) for sperm motility in 4h (18.8%) and 12h (17.4%) of balance, but there was an increase in 8h (29.6%) (p <0.05). In relation to the force and the detachment of the acrosome was no statistical difference (p> 0.05) 4h (2.0 and 35.8), 8h (2.1 and 33.4) and 12h (1.8 and 42 , 2) equilibrium. On cryopreservation with PBS, showed no motility and vigor after thawing. These results indicate that solvent-based TES and the equilibrium time of 8h were more suitable for dilution and freezing of ram semen.
Acesso aberto (Open Access)
Avaliação nutricional da torta de dendê para suplementação de ruminantes na Amazônia Oriental
(Universidade Federal do Pará, 2006) COSTA, Dayana Alves da; CAMARÃO, Ari Pinheiro; http://lattes.cnpq.br/1649891765946593; LOURENÇO JÚNIOR, José de Brito; http://lattes.cnpq.br/2919433679918544
The study was carried out at the Eastern Amazon Research Center of Embrapa, in Belem, Para State, Brazil (1º28'S 48º27'W of Greenwich), with the objective of evaluate the influence of the addition of the palm kernel cake (Elaeis guineensis) as alternative feed supplementation of ruminants, in the critical periods of forage production of in the Amazon Eastern. The nutritional characteristics of palm kernel cake was determined during twenty one days, using sixteen sheep, in individual metabolic cage, distributed in a completely randomized experimental design, with four treatments and four replications. The experimental rations (T1, T2, T3 and T4) contained Brachiaria humidicola and palm kernel cake, at four levels (10%, 20%, 30% and 40%). The consumption of dry matter (g/day) and % of live weight were 666.6 and 2.5; 686.9 and 2.4; 649.4 and 2.4; e 540.9 and 2.0, of organic matter 706.5; 710.8; 708.1 and 632.3 g/day, and of crude protein 37.3; 42.9; 58.7 and 56.4 g/day. The fiber in neutral detergent consumption in g/day, were 584.7; 583.5; 565.2 and 527.0. The coefficients of digestibility were 50.3; 47.8; 52.3; and 55.2%, in dry matter basis, and 50.8; 49.6; 53.5; and 56.3%, in organic matter basis, and the crude protein contents were 48.0; 38.7; 66.8; 69.4%, in T1, T2, T3 and T4, respectively. The palm kernel cake shows good feeding potential, with high dry matter and good nutritional value, being considered as an alternative for feeding supplementation for ruminants, mainly at the level of 30%, and allows greater consumption and digestibility of dry matter, organic matter, crude protein, with adequate supplement of energy.
Acesso aberto (Open Access)
Brachiaria spp. poisoning of ruminants in Brazil
(2011-03) RIVERO, Beatriz Riet Correa; CASTRO, Márcio Botelho de; LEMOS, Ricardo Antônio Amaral de; CORREA, Gabriela Riet; MUSTAFA, Vanessa da Silva; CORREA, Franklin Riet
Brachiaria species are the most important grasses for cattle production in Brazil. However, a limiting factor for the use of Brachiaria spp. is their toxicity. Most outbreaks of hepatogenous photosensitization are caused by B. decumbens; however B. brizantha, B. humidicola and B. ruziziensis can also cause poisoning. The poisoning affects cattle, sheep, goats and buffalo. Sheep are more susceptible than other animal species and the young are more susceptible than adults. There are differences in susceptibility among animals of the same species and it has been suggested that this resistance is genetic. Also has been suggested that buffalo and probably some sheep are resilient, i.e. when poisoned these animals have histologic lesions and high GGT serum concentrations, but do not show clinical signs. In general, saponin concentrations are higher in growing plants, but outbreaks occur all over the year, probably due to unexplained rise in saponin concentration in the plant. A clinical syndrome of progressive weight loss and death, without photosensitization, has been reported in cattle poisoned by B. decumbens. Main preventive measures are based on the selection of resistant or resilient animals and on the development of Brachiaria species or varieties with low saponin concentration.
Acesso aberto (Open Access)
Consumo, digestibilidade, balanço de nitrogênio e composição bromatológica da torta de murumuru (Astrocaryum murumuru var. murumuru Mart.), na alimentação de ruminantes
(Universidade Federal do Pará, 2012-02-10) MENEZES, Bruno Peres de; SILVA, André Guimarães Maciel e; http://lattes.cnpq.br/8940750096354420; http://lattes.cnpq.br/9647377426235964; ANDRADE, Stefano Juliano Tavares de; LOURENÇO JÚNIOR, José de Brito; http://lattes.cnpq.br/2919433679918544
We assessed the pie murumuru (Astrocaryum murumuru var. murumuru, Mart.), from processing kernels after oil extraction by the cosmetic industry, the effects of the substitution levels of 0%, 10%, 20%, 40% and 60% in the basic diet of Mombaça grass (Panicum maximum cv. Mombaça) on intake and digestibility of dry matter (CDDM), organic matter (CDOM), ether extract (CDEE), crude protein (CDCP), fiber neutral detergent (CDFND), fiber acid detergent (CDFDA), cellulose (CDCEL) and hemicellulose (CDHCEL) and nitrogen balance. Metabolism trial was conducted with 20 castrated sheep, live weight of 24 kg, distributed in five treatments and four replications in a completely randomized design. Adaptation period was 21 days with five days of data collection of diet and refusals, feces and urine. The CMS, CMO, EEC, CCP, FND, CFDA, CHCEL, CLIG showed a quadratic effect as a function of the substitution with pie in the diet. The CDDM, and CDCP CDMO showed no significant effects. The CDEE with optimal replacement level 56,64% and 88,62% maximum digestibility, CDFND with optimal replacement level 42,45% and 68,25% maximum digestibility, CDFDA with optimal replacement level of 31,63% and maximum digestibility of 66,80%, CDCEL with optimal replacement level 27,45% and 72,21% maximum digestibility showed a quadratic effect due to substitution murumuru pie. Nitrogen balance had no significant effect of inclusion in the grass of 0% to 60% of pie. It is concluded that the pie murumuru (Astrocaryum murumuru var.Murumuru Mart.), Has as its main limitation is the low dietary intake and digestibility, in addition to impossibility of its use as a concentrate exclusively on the diet of sheep showing up as an alternative food for up to 20% replacement in diets.
Acesso aberto (Open Access)
Dermatite alérgica à picada de insetos em ovinos no estado do Pará
(2011-02) BARBOSA NETO, José Diomedes; ALBERNAZ, Tatiane Teles; OLIVEIRA, Carlos Magno Chaves; DUARTE, Marcos Dutra; OLIVEIRA, Cairo Henrique Sousa de; BRITO, Marilene de Farias; SILVA, André Guimarães Maciel e
A skin disease was studied in 159 sheep on 15 farms located in seven counties of northeastern and southeastern of the state of Pará and in one farm in the state of Roraima, Brazil. The disease affected mainly two to four year-old sheep of the Santa Ines breed and crossbreds. Clinical signs were characterized by alopecia, multifocal erythema, small papules, and crusts in several areas of the body, mainly around the eyes. Severe itching in the affected areas, restless, weight loss and lacrimation were also observed. Insects were always seen around the animals. Insects apparently associated with the lesions were captured and identified as pertaining to the genera Simulium and Hippelates. Biopsies of the skin lesions of 10 sheep revealed inflammatory non suppurative mononuclear infiltrates of the dermis with presence of eosinophils. The epidemiologic, clinical and pathological studies of the cases allowed the diagnosis of allergic dermatitis due to the insect bites.
Acesso aberto (Open Access)
Desempenho e características de carcaças de cordeiros suplementados com diferentes níveis de resíduo de biodiesel
(Universidade Federal do Pará, 2011-02-15) SOARES, Bruno Cabral; ÁVILA, Sandra Cristina de; http://lattes.cnpq.br/9683521652094464; http://lattes.cnpq.br/8940750096354420; SILVA, André Guimarães Maciel e; LOURENÇO JÚNIOR, José de Brito; http://lattes.cnpq.br/2919433679918544
Was aimed verify the effect from different levels of supplementation from biodiesel residue originating from dendê. Were utilized 25 male ovines, Mutt, gelded, with middleweight live of 20 kg. The experimental delineation utilized were randomized blocks, with 5 replicates per treatment, for the weight gain data and carcass characteristics. The biometrics data were analyzed in a subdivided parcel scheme. The experimental period was 84 days, with 14 days of adaptation and 70 days to obtain study variables. The feeding was split in 2 meals, at 7h00 and 17h00. Diets were composed 34% hay Panicum maximum cv. Massai and 61% concetrated, from broken corn, soy bran, minerals and 5% of dendê oil or dendê biodiesel residues, on increasing levels (zero, 25,50,75 and 100%), respectively. At the beginning of the trial period and each 14 days were realized morning weighings, before the first meal for performance evaluation, biometric measures and slaughtered on final of the trial period for carcass characteristics evaluation after passing 12h fasting of solids and liquid diet. Significant effects were observed (p<0,05) from inclusion levels of biodiesel residue originating from dendê. The regression equations presented increasing linear effect, for variables: dry matter consumption (DMC), live weight (LW), weight gain (WG), body score (BS), body length (BL), thoracic perimeter (TP), chest width (CW), and quadratic for PCQ, PCF,RCB, RB. Dendê biodiesel residues utilization, in replace of dendê oil on lambs food, promotes increasing effect on dry matter consumption and weight gain, improves carcass characteristics and becomes an alternative to increase energetic density of growing ovines diet.
Acesso aberto (Open Access)
Desempenho e características de carcaças de cordeiros suplementados com diferentes níveis de resíduo de biodiesel
(2012-12) SOARES, Bruno Cabral; SOUZA, Karla Débora Santana de; LOURENÇO JÚNIOR, José de Brito; SILVA, André Guimarães Maciel e; ÁVILA, Sandra Cristina de; KUSS, Fernando; ANDRADE, Stefano Juliano Tavares de; RAIOL, Laura Cristina Barra; COLODO, Juliana Cristina Nogueira
Diet supplementation with biodiesel residues of confined lambs, utilizing 25 sheep, without definite breed pattern (WDB), male, castrated, with average weight of 20kg was studied. The experimental design was random blocks, with 5 repetitions per treatment and a split plot scheme toward biometrics data. The experiment lasted 70 days. The diets were composed of 34% of roughage and 61% of concentrate, based on corn, soy, mineral and 5% of oil palm kernel or biodiesel residues, in increasing percentages – zero, 25, 50, 75 and 100%. The animals were slaughtered at the end of the experiment for carcass evaluation. Significant effects (P<0.05) of percentage inclusion of residues were observed for the performance and biometrics variables demonstrated in the crescent linear effect of the regression equations, as well as for the hot carcass weight (HCW) – with an average of 14; 15.92; 16.14; 16.42 and 18.02%; and the cold carcass weight (CCW) – with an average of 13.12; 14.78; 15.06; 15.70 and 17.25% for diets, respectively, containing 0, 25, 50, 75, 100% of palm kernel biodiesel residues. The palm kernel biodiesel residue utilization in feeding growing lambs is an alternative to increase the energetic density in their diets.
Acesso aberto (Open Access)
Dinâmica celular e microbiológica do leite de ovelhas Santa Inês acompanhadas durante a lactação
(2011-10) GUARANÁ, Eduardo Levi de Sousa; SANTOS, Rogério Adriano dos; CAMPOS, Anne Grace Silva Siqueira; SILVA, Natália da Silva e; AFONSO, José Augusto Bastos; MENDONÇA, Carla Lopes de
The aim of this study was to evaluate the dynamics of intramammary infection in ewes by means of clinical examination, direct somatic cell count and the isolation of bacterial agents involved in the process during lactation, as well as the sensitivity profile of these isolates to different antimicrobials. Thirty four ewes from the Santa Inês breed, raised under semi-intensive conditions and submitted to identical sanitary and nutritional management were evaluated before and after parturition, about 10 days before parturition, 15 days after parturition (dap), 30 dap, 60 dap and 90 dap (weaning). In the respective phases a clinical exam of the mammary gland was accomplished. The direct somatic cell count (SCC) and the California Mastitis Test (CMT) were carried out in the phases postpartum (15 dap, 30 dap, 60 dap and 90 dap) as well as the bacteriological analysis which was also accomplished in the experimental phase that preceded parturition when the ewes were not milked. Milk sampling was achieved by manual means. All ewes were submitted to lentivirus serology test. The variable data of SCC were tested for normality according to Kolmogorov-Smirnov and not attended the premise of normality were transformed into log base 10 (Log10). Therefore was performed the analysis of variance and contrast of means by Tukey test with significance level of P<0.05. A descriptive study of the variables studied was done by frequency distribution (%). The average somatic cell count in negative reactions to the CMT varied 387.896,08 cells/mL to 620.611,11 cells/mL and glands reagents ranged up to 6.730.514,50 cells/mL, but there was no influence in the different stages of lactation. The results allowed to conclude that subclinical mastitis represents a sanitary concern in the breeding of Santa Ines ewes. The phase that precedes parturition is particularly worrying and deserves more attention since there is a higher bacterial isolation percentage in apparently healthy glands. In association, it was also in the first 30 days of lactation (initial phase) that a high frequency of bacterial isolation was perceived, with coagulase-negative Staphylococcus as the agent isolated in higher percentage.
Acesso aberto (Open Access)
Epidemiological, clinical and pathological aspects of an outbreak of periodontitis in sheep
(Universidade Federal do Pará, 2016-11) SILVA, Natália da Silva e; SILVEIRA, José Alcides Sarmento da; LIMA, Danillo Henrique da Silva; BOMJARDIM, Henrique dos Anjos; BRITO, Marilene de Farias; BORSANELLI, Ana Carolina; DUTRA, Iveraldo dos Santos; BARBOSA NETO, José Diomedes
The objective of this study was to describe the epidemiological, clinical and pathological aspects of an outbreak of periodontitis in sheep that occurred on a rural property in Benevides, in the state of Pará, Brazil. The first clinical signs of this disease in the herd - visible nodular swollen mandible - were observed about one month after the sheep began grazing on a recently renovated Panicum maximum cv. Massai pasture; the animals were also supplemented with elephant grass (Pennisetum purpureum). From the 545 adult Santa Ines, Dorper, Texel and crossbred sheep, 20 (3.7%) showed facial bulging, particularly of the mandible. These 20 sheep, which were generally over 36 months of age, had low body condition scores as well as dull and ruffled coats; many of them had loose or missing pre, upper or lower molars, showed the formation of abscesses and fistulas, exhibited pain upon palpation and had difficulty chewing. In the same herd, the prevalence of periodontal lesions at the premolars and molar teeth was evaluated via post-mortem examination of 39 young and 17 adult sheep. In 51.3% (20/39) of adolescents and in 100% of adults, periodontal lesions were found at least at one tooth of the dental arch, with unilateral or bilateral periodontal lesions at the maxillary and mandibular teeth. Histopathological analysis of 13 sheep revealed pyogranulomatous inflammation. The second and third maxillary premolars were the teeth that were most affected in young animals, whereas the third pre-molar and the molars, of the maxilla and mandible, were most often affected in adults. These lesions led to severe bone destruction, alterations in the dental arch, occlusion, tooth loss and periodontal abscesses. The epidemiological and clinic-pathological characteristics of this periodontitis outbreak, described here for the first time in sheep in Brazil, were similar to those for bovine periodontitis (“cara inchada”).
Acesso aberto (Open Access)
Etiologia e perfil de sensibilidade de bactérias isoladas de ovelhas com mastite na região Nordeste do estado do Pará
(2010-12) SILVA, Natália da Silva e; SILVEIRA, José Alcides Sarmento da; PINHEIRO, Cleyton Prado; SOUSA, Melina Garcia Saraiva de; OLIVEIRA, Carlos Magno Chaves; MENDONÇA, Carla Lopes de; DUARTE, Marcos Dutra; BARBOSA NETO, José Diomedes
The objective of this paper was to study the etiology of mastitis in sheep at northeastern Pará, and to establish the sensitivity of isolated bacteria to antibiotics. A total of 176 Santa Inês nursing sheep kept in semi-intensive system from seven properties were examined. The mammary gland was clinically examined and the milk was submitted to the Caneca Telada Test, the California Mastitis Test (CMT), bacteriological examinations and antibiograms. Out of the 352 mammary halves (176 sheep), 5.9% (21/352) had clinical mastitis and by the CMT test, 7.39% (26/352) had subclinical mastitis and 86.64% (305/352) mammary halves did not have mastitis. Most of the animals with mastitis were in the second third of the lactation period, had less kids and more lactation periods. The following bacteria were isolated from the clinical mastitis Staphylococcus spp. coagulase negative (42,9%); Staphylococcus aureus (9.52%); Streptococcus spp. (4.76%) and Escherichia coli (4.76%). Were observed associations of Staphylococcus aureus and Streptococcus spp. (4,76%); Staphylococcus spp. coagulase negative nonhemolytic, Staphylococcus spp. coagulase negative hemolytic and Staphylococcus spp. coagulase negative non hemolytic pigment (4.76%). Already in subclinical mastitis the bacteria isolated were Staphylococcus spp. coagulase negative (26.9%); Staphylococcus aureus (15.,4%); Streptococcus spp. (7.69%); Escherichia coli (7.69%) and Citrobacter freundii (11.5%). Were observed associations of Staphylococcus spp. coagulase negative nonhemolytic and Staphylococcus spp. coagulase negative hemolytic (3.85%). The most efficient antibiotics for the Gram positive agents were penicile/novobiocine (100%), cefalotine (100%) and florfenicol (100%) and for the Citrobacter freundii were ampicilina (100%) and florfenicol (100%). In relation to Escherichia coli, 66.7% of isolates to ampicillin, cephalothin, florfenicol and tetracycline were resistant. Mastitis is present in sheep in the State of Pará, and it's necessary to estimate, in future studies, the economic losses caused by this disease. The CMT show satisfactory results and can be recommended as a screening test for diagnosing individual cases of subclinical mastitis in sheep, once had a good relationship with the microbiological examination. In the antibiogram where most of the isolated agents appear sensitive to different antibiotics tested, the antibiotics with the best efficiency were florfenicol and cefoxitin.
Acesso aberto (Open Access)
Evolução e reversibilidade das lesões neurológicas e cardíacas em ovinos intoxicados experimentalmente por Ateleia glazioviana e Tetrapterys multiglandulosa
(2008-03) ALMEIDA, Milton Begeres de; PRIEBE, Amanda Pantaleão da Silva; RIVERO, Beatriz Riet Correa; CORREA, Gabriela Riet; FISS, Letícia; RAFFI, Margarida Buss; SCHILD, Ana Lucia
To determine the reversibility of neurological and cardiac lesions in Ateleia glazioviana and Tetrapterys multiglandulosa poisoning, 3 groups of four sheep each were fed orally with the plants. In Group 1, 2 and 3, when sheep with nervous signs showed considerable increase of symptoms and risk of death, the plant administration was suspended. Group 4 with two sheep was used as control. Sheep from Group 1, fed 10g/kg during 6 days of fresh A. glazioviana collected in autumn were euthanized 8, 11, 16, and 21 days after the start of the experiment; they had shown regression of nervous signs, but had progressive cardiac lesions. Sheep from Group 2, fed during 8 days the same dose of fresh A. glazioviana collected in spring, were euthanized on days 9, 23, 38 and 68; they had shown regression of nervous signs and had no cardiac lesions. This suggests that the plant is less toxic in spring. Sheep from Group 3, fed 10g/kg during 11 days dry and ground T. multiglandulosa mixed with concentrated food, were euthanized on days 33, 33, 92 and 92; they had shown regression of nervous signs, and cardiac lesions were less severe on day 33 than on day 92. These results indicate that nervous lesions are reversible after the end of feeding, but cardiac lesions are progressive after discontinuation of the plant administration; these can be afterwards reversible if the animals do not anymore show clinical signs or die as a consequence of the poisoning. Results of those and previous experiments show that lesions of the nervous system are induced by lower doses than cardiac lesions and occur within a shorter period, what suggests that in spontaneous cases cardiac signs are always preceded by nervous signs, and that nervous signs can occur in the absence of cardiac lesions.
Acesso aberto (Open Access)
Feeding preferences of experienced and naïve goats and sheep for the toxic plant Ipomoea carnea subsp. fistulosa
(Universidade Federal do Pará, 2015-09) OLIVEIRA JÚNIOR, Carlos Alberto de; CORREA, Gabriela Riet; LIMA, Everton Ferreira; LEITE, Danilo Maia; PFISTER, James A.; COOK, Daniel; CORREA, Franklin Riet
Ipomoea carnea is a toxic plant that grazing goats and cattle may learn to ingest with repeated exposure. The objective of this study was to evaluate the feeding preferences of experienced and non-experienced (naïve) goats and sheep for I. carnea. The study used 3 groups of 5 goats (Group 1, experienced that were previously poisoned by the plant; group 2, naïve; Group 3, experienced eaters, composed of animals adapted to eat the fresh plant) and 2 groups of sheep (group 4, experienced that were previously poisoned by the plant; and group 5, naïve). For the test, the animals were placed daily for 10 minutes and 4 days in a rectangular stall (5x7m) with 4 feeders, each with 200g of a different food (Ipomoea carnea, commercial concentrate food, recently harvested green grass (mainly Brachiaria spp.), and Cynodon dactylonhay. The intake of concentrate food was significantly higher (P<0.05) than the consumption of green grass, hay and I. carnea. In a second 4 day trial, in which the commercial concentrate food was replaced by freshly harvested Amorimia septentrionalis, the ingestion of green grass (Brachiaria spp.) was significantly higher (P<0.05) than the consumption of other foods. In both trials there was no significant difference in food consumption between eaters and naïve animals. The results suggest that experienced or naïve sheep and goats do not prefer I. carnea when it is offered with other foods or forages, suggesting that animals will avoid the plant and not become poisoned if other food options are available.
Acesso aberto (Open Access)
Fotossensibilização em ovinos associada à ingestão de Brachiaria brizantha no estado do Pará
(2010-09) ALBERNAZ, Tatiane Teles; SILVEIRA, José Alcides Sarmento da; SILVA, Natália da Silva e; OLIVEIRA, Cairo Henrique Sousa de; REIS, Alessandra dos Santos Belo; OLIVEIRA, Carlos Magno Chaves; DUARTE, Marcos Dutra; BARBOSA NETO, José Diomedes
Two outbreaks of photosensitization by Brachiaria brizantha were studied and an experiment was performed in Santa Inês and Dorper crossbred sheep, two to three months old, on a farm in Santa Luzia do Pará municipality. These animals were kept from birth until about two months of age in a suspended stall floor, fed purple elephant grass (Pennisetum purpureum cv. purple), and had minerals and water ad libitum. After this period they were placed into a paddock of B. brizantha. At the time of the outbreaks and the experimental studies, the farm was visited for epidemiological assessment and clinical examination of the sheep, collection of blood samples for measurement of gamma glutamyltransferase, aspartate aminotransferase, conjugated bilirubin, unconjugated bilirubin, urea, and creatinine. Pasture samples were collected for saponin determination and count of Pithomyces chartarum spores. Necropsies with collection of material for histopathological studies were performed. Outbreak 1 occurred at the time of low rainfall, when the grass was scarce and mature, and morbidity and lethality was 43.4% and 81.6%, respectively. Outbreak 2 occurred at the beginning of the rainy season, with morbidity and lethality rates of 16.3% and 76.9%, respectively. From the 50 animals in the experiment, 10 received three times 200ml of rumen fluid taken from mother sheep of the same batch. The first of these administrations was given one day before the sheep were introduced into the pasture and the other two at weekly intervals. After 15 days in the pasture, the animals were unquiet, looked for shade, had edema of the ears, yellowish mucosae, were apathic, had anorexia and showed sloughing of the skin followed by crusting in some areas of the body. Both, the sheep of the outbreaks as those from the experiment, showed increased levels of GGT, AST, BD, BI, BT, urea and creatinine. In sheep which received ruminal fluid the values of urea and GGT were similar to those which did not receive the ruminal fluid. The creatinine, AST and bilirrubine values were lower in sheep that received the ruminal fluid. Two types of saponins, methylprotodioscin and protodioscin, were detected in the samples of B. brizantha from the outbreaks and the experiment. The level of saponins in Outbreak 1 and 2 was 0.92% and 0.88% respectively. The levels of saponins in the experiment ranged from 1.13% to 1.62%. The numbers of spores of Pithomyces chartarum in both outbreaks and in the experiment were negligible. At necropsy there was widespread jaundice, the liver was brown-yellow with marked lobular pattern and increased consistency and the kidneys were enlarged yellowish-green. Histological changes occurred mainly in the liver and consisted of mild proliferation of bile ducts in portal tracts, presence of binucleate hepatocytes, foamy macrophages, necrosis of isolated hepatocytes, cholangitis, presence of crystals in hepatocytes and macrophages.
Acesso aberto (Open Access)
Intervalos de referência sanguíneos e a influência da idade e sexo sobre parâmetros hematológicos e bioquímicos de ovinos da raça Santa Inês criados na Amazônia Oriental
(Instituto Nacional de Pesquisas da Amazônia, 2015-09) LIMA, Misael Brito de; MONTEIRO, Maria Vivina Barros; JORGE, Ediene Moura; CAMPELLO, Claudio Cabral; RODRIGUES, Luiz Fernando Souza; VIANA, Rinaldo Batista; MONTEIRO, Frederico Ozanan Barros; COSTA, Cícero Temístocles Coutinho
Blood count and biochemical tests are used to diagnose diseases in domestic animals. These tests can be influenced by age, gender, nutrition, breed, species and environmental conditions. Thus, data from one region should not be extrapolated to animals raised in other regions. The objective of this study was to determine the hematological and biochemical values of different ages and genders of Santa Inês sheep, raised in the eastern Amazon. There were examined 91 sheep that were assigned to three groups: G1 (three to six months old, n = 31); G2 (seven to 24 months old, n = 30) and G3 (above 24 months old, n = 30). The blood cell count, white blood count and biochemical determinations were made with an automatic counter and a semi-automatic analyzer, respectively. Mean values were compared using the Tukey test. The number of erythrocytes, the red blood indices, the thrombogram, eosinophils, total protein, urea and creatinine concentrations were influenced by the age of the animals. The erythrocyte coefficient of variation and the creatinine concentration were influenced by gender, and were greater in males. The neutrophil:lymphocyte (N:L) ratio was greater than one for all age groups. This study led to the determination of reference values for sheep raised in the Eastern Amazon and demonstrated that when interpreting hematological and biochemical tests of sheep, age and gender must be considered.
Acesso aberto (Open Access)
Intoxicação experimental por Tetrapterys multiglandulosa (Malpighiaceae) em ovinos
(2005-06) CORREA, Gabriela Riet; TERRA, Fabiano Fonseca; SCHILD, Ana Lucia; CORREA, Franklin Riet; BARROS, Severo Sales de
Cardiac fibrosis was observed in a calf showing dullness, weakening and respiratory insufficiency in a farm in the state of São Paulo, where cardiac insufficiency, abortion and nervous signs in cattle were associated with the ingestion of Tetrapterys multiglandulosa. The objectives of this paper were to determine the susceptibility of sheep to the intoxication by T. multiglandulosa, to describe the clinical and pathological characteristics of the intoxication, and to evaluate the possibility of using sheep as an experimental species for toxicological studies with this plant. In a previous experiment to determine the toxicity of T. multiglandulosa to be used in sheep, the green plant was given to a steer at the dose of 22g per kg body weight (g/kg/bw), daily, during 9 days. After 9 days the steer showed nervous signs, and on the 12thday was euthanatized. No gross lesions were observed at necropsy. Status spongiosus was observed on the deeper layers of the cerebral cortex and subcortical white matter. Six male sheep were divided in three groups of two sheep each. Group 1 (Sheep 1 and 2) received daily doses of 6 g/kg/bw of the dry plant, during 30 days; Group 2 (Sheep 3 and 4) received daily doses of 3 g/kg/bw, during 60 days; and Group 3 was the control group. Sheep 1 was euthanatized 30 days after the start of the ingestion. Only cardiac arrhythmia was observed clinically, and no lesions were observed at necropsy. Sheep 2, 3 and 4 had also cardiac arrhythmia from day 9, 12 and 18, respectively. From day 52 they started to show depression, reluctance to move and incoordination. Clinical signs got gradually worst and the sheep were euthanatized on days 60, 70 and 80, when clinical signs were marked, and the animals will die at any moment. Hydrothorax, hydropericardium, ascites, nutmeg appearance of the liver and hard whitish myocardium, mainly in the interventricular septum and left ventricle, were observed at necropsy. Histologically, the heart of Sheep 2, 3 and 4 had areas of fibrosis associated with mononuclear cell infiltration. No lesions were found in the heart of Sheep 1. The 4 treated sheep had status spongiosus in different areas of the cerebrum and brain stem, mainly of the deep layers of the cerebral cortex and subcortical white matter. Status spongiosus were also observed in the cerebellar white matter and cervical medulla. In the later the vacuolization was moderate in the white matter and mild in the grey matter. Status spongiosus were mild in Sheep 1 and moderate to severe in Sheep 2, 3 and 4. On electron microscopy it was observed that the status spongiosus is due to an intramyelinic edema. No gross or histologic lesions were observed on the two control sheep, which were euthanized on day 80 after the start of the experiment.
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Intoxicação por Ipomoea asarifolia em ovinos e bovinos na Ilha de Marajó
(2008-12) TORTELLI, Fábio Py; BARBOSA NETO, José Diomedes; OLIVEIRA, Carlos Magno Chaves; DUARTE, Marcos Dutra; CERQUEIRA, Valíria Duarte; OLIVEIRA JÚNIOR, Carlos Alberto de; CORREA, Franklin Riet; RIVERO, Gabriela Riet Correa
To determine plant poisonings occurring on Marajo Island, state of Pará, northern Brazil, 7 farms were visited. All farms had native pastures with low forage availability severely infected by Ipomoea asarifolia. In 5 farms poisoning in sheep by the plant was reported. The disease occurs mainly during the dry period and lambs are more frequently affected than adult sheep. Sheep with clinical signs were observed in 2 farms. In 4 establishments farmers reported the occurrence of I. asarifolia poisoning in cattle. In one farm affected cattle were observed during two visits, one during the dry season and another at the start of the raining season. Deaths are observed mainly in calves. In both species the poisoning occurs every year with variable morbidity and low mortality. One bovine and one sheep with severe clinical signs were euthanized and post-mortem examination was performed. No gross or histologic significant lesions were observed. In all farms buffaloes were raised together with cattle and sheep, and in 3 farms goats were also raised. None of the farmers reported the intoxication in these species.
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Intoxicação por Ipomoea spp. em ruminantes na Ilha de Marajó
(Universidade Federal do Pará, 2009-02-09) TORTELLI, Fábio Py; RIVERO, Gabriela Riet Correa; http://lattes.cnpq.br/7540534565167837
Acesso aberto (Open Access)
Intoxicações experimental e natural por Ipomoea asarifolia (Convolvulaceae) em búfalos e outros ruminantes
(2005-12) BARBOSA NETO, José Diomedes; OLIVEIRA, Carlos Magno Chaves; DUARTE, Marcos Dutra; PEIXOTO, Paulo Fernando de Vargas; TOKARNIA, Carlos Maria Antônio Hubinger
Ipomoea asarifolia R. et Schult. is a plant responsible for occasional outbreaks of poisoning in cattle, sheep and goats. Its oral administration to cattle and buffalos showed that the buffalo is as susceptible as cattle. One to 4 daily doses of 10-20g/kg caused similar symptoms in both species, as incoordination, muscular twiching and swaying of the head and anterior part of the body (pendular movement). In buffaloes the signs of incoordination were observed to a smaller extent. There is no report yet on natural poisoning by I. asarifolia in buffaloes, possibly because cases are not being noticed.
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Lesões de pele causadas pelos espinhos de Mimosa pudica (Leg. Mimosoideae) nos membros de bovinos e ovinos no estado do Pará
(2009-05) BARBOSA NETO, José Diomedes; SILVEIRA, José Alcides Sarmento da; ALBERNAZ, Tatiane Teles; SILVA, Natália da Silva e; REIS, Alessandra dos Santos Belo; OLIVEIRA, Carlos Magno Chaves; RIVERO, Gabriela Riet Correa; DUARTE, Marcos Dutra
A condition of cattle and sheep characterized by ulcerous and granulomatous skin lesions of the legs was studied. Epidemiological data and the nature of the lesions indicate that they are caused by the thorns of Mimosa pudica (Leg. Mimosoideae). The disease was observed only in pastures largely infested by the plant, and animals recover quickly when moved from them.
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Potencial nutritivo da torta de dendê na alimentação de ruminantes no estado do Pará
(Universidade Federal do Pará, 2010-06-23) VASCONCELOS, Helbilena Gildeli Rodrigues; ÁVILA, Sandra Cristina de; http://lattes.cnpq.br/9683521652094464; FATURI, Cristian; http://lattes.cnpq.br/8389692203753236
Assesses the effects of inclusion of palm kernel cake in the diet of growing sheep reared under tropical conditions, with emphasis on effects on intake, digestibility and performance of ruminants. For testing the intake and digestibility was used 16 Santa Inês sheep, with a mean age of 3 months and weighing approximately 19 kg during 19 days, 14 of adaptation and five days of collecting data and samples from metabolic cages. And for performance, with 15 male 4 months old, the same breed, weighing approximately 20 kg for 56 days, distributed in a randomized blocks with four treatments: 0, 25, 50 and 75% inclusion of palm kernel cake in the concentrate, diets composed of 40% (dry matter basis) of silage, elephant grass (Pennisetum purpureum) and 60% concentrate consisting of corn, soybean, palm kernel cake and minerals. The result showed a linear reduction in dry matter intake with the inclusion of palm kernel cake, but there was no statistically significant difference in dry matter digestibility. It was also noted an increase in digestibility of NDF and ADF in diets with higher proportions of palm kernel cake. In the performance test, there was a linear reduction in weight gain of animals as it raised the level of palm kernel cake in the diet, the result of lower dry matter intake (p <0.05) and worse feed conversion. The palm kernel cake is potentially productive, with high dry matter availability and good nutritional value. It is a good alternative to be used as a food supplement for sheep, but at higher levels can reduce animal performance, and its use depends on the purchase price and cost: benefit.