Navegando por Assunto "Phytochemistry"

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Acesso aberto (Open Access)
Investigação fitoquímica e avaliação da atividade anti inflamatória do extrato hidroetílico de folhas de salix humboldtiana em modelo zebrafish (Danio rerio)
(Universidade Federal do Pará, 2021-09-25) RAMOS, Uêbem Fernandes; BARBOSA, Wagner Luiz Ramos; http://lattes.cnpq.br/1372405563294070; https://orcid.org/ 0000-0002-2421-8245
The leaves of Salix humboldtiana, family Salicaceae, are used in traditional medicine for the treatment of various inflammatory diseases, febrile states, among others. However, there is little information about the species in the literature. However, the genus Salix has a very significant diversity of uses in popular medicine, with Salix alba L. as its most renowned representative, which is included in the Brazilian Pharmacopoeia Form of Herbal Medicine. This work proposes to investigate chemical constituents with an emphasis on phenolic compounds, through pharmacognostic studies (analysis of physical-chemical quality); the phytochemical approach, the tests, served to demonstrate the presence of classes of secondary metabolites: reducing sugars, phenols and tannins, flavonoids, catechins, steroids and coumarin derivatives; fractionation, CCD and CLAE-DAD. In addition to biological testing on an animal model (D. rerio) to investigate anti-inflammatory activities. In this sense, the objectives of performing the phytochemical analyzes and the bioassay, the Salix humboldtiana Ethyl Extract (EBSh) was used in different doses in in vivo tests. Groups previously treated with 75 mg / kg EBSh showed 5% inhibition; 150 mg / kg showed 14% inhibition; 300 mg / kg showed 54% inhibition; 500 mg / kg showed 60% inhibition, 750 mg / kg showed 43% inhibition of abdominal edema compared to the negative control. Among the five different concentrations of EBSh tested and evaluated in the zebrafish model, the concentration of 500 mg, provided a statistically more expressive response regarding anti-inflammatory activity and anti-edematogenic degree after 5 h of treatment. This response was superior to the indomethacin standard when compared to the negative control, thus indicating the ability to reduce and / or inhibit edema in the peritoneal region. The animals subjected to the tests showed changes in normal behavior at all doses tested, these changes varied according to the concentration of EBSh administered. In the three lowest doses (75 mg, 150 mg and 300 mg), the fish showed only mild to moderate changes and less frequently, while in the two highest concentrations (500 mg and 750 mg), they manifested important behavioral changes, some with greater frequency and intensity.
Acesso aberto (Open Access)
Principais compostos bioativos e capacidade antioxidante no epicarpo do camu-camu (Myrciaria dubia) em função da maturação
(Universidade Federal do Pará, 2014-06-25) SOUZA, Aline Ozana de; MATTIETTO, Rafaella de Andrade; http://lattes.cnpq.br/7832266671782588; LOPES, Alessandra Santos; http://lattes.cnpq.br/8156697119235191
The presence of bioactive compounds suffers greatly influenced by environmental and genetic factors, and behave differently in each part of the plant. However, there are few studies which deal these aspects of camu-camu. Thus, this study aimed to analyze the physical, physico-chemical composition, the main bioactive compounds and antioxidant capacity of camu-camu bark, depending on the stage of ripeness in fruits of different genotypes from plant matrices Bank Active Germplasm Camucamuzeiro of Embrapa Eastern Amazon. Fruits of three genotypes, selected at random in three maturity stages were collected. Results were expressed on a dry basis and analyzed by Analysis of Variance, Comparison Test Tukey's and Pearson's Correlation Coefficient, with a confidence interval of 95%. For physico-chemical analysis, there was a different one for each progeny, depending on maturity, except that the carbohydrate content increased in all progenies behavior. For vitamin C, the progenies 38 and 44 showed a high content of ascorbic acid, comparing the green and ripe stages, and 17 progeny showed a decrease during maturation; the highest content of vitamin C has been found in 44 progeny, ripe stage (24,02 g/100g). All progenies showed an increase in the content of total phenolics and anthocyanins throughout maturation, with the highest values were demonstrated by progeny 17 (3298,98 AGE/100g mg) and 44 (165,91 mg/100g) respectively. Flavonols and carotenoids showed a distinct and statistically different behavior in each progeny, with the highest content of flavonols was found in 17 progeny in the green stage (343,63 mg QE/100g) and carotenoids in 44 progeny, ripe stage (105,88 mg/100g). Regarding the antioxidant activity based on the DPPH method, the progenies 38 and 44 showed the same behavior throughout maturation, with higher antioxidant activity half ripe and smallest stage in green, with the highest antioxidant activity was found in 17 progeny in the ripe stage (38,95 g fruit/g). Already by ABTS method, the antioxidant activity was statistically different for all progenies and in all stages of maturation, higher value submitted by 44 progeny at maturity (170,63 μM trolox/g). Evaluating the relationship between bioactive compounds and antioxidant activity, the 17 progeny won more statistically significant correlations. It can be concluded that various factors cause differences in the synthesis of various compounds during fruit ripening of camucamuzeiro.