Navegando por Assunto "Piper aduncum L."

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Acesso aberto (Open Access)
Atividade antimicrobiana do óleo essencial de Piper aduncum L. e seu componente, dilapiol, frente a Staphylococcus spp. multirresistentes
(Universidade Federal do Pará, 2012-08-31) BRAZÃO, Maria Angélica Bolini; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390
The essential oil of Piper aduncum (EPO) and its main component, the dilapiol (76.5%), were evaluated for antibacterial activity against different strains of Staphylococcus spp. ATCC and multiresistant. For testing the antibacterial activity of EPO and dilapiol were determined Minimum Inhibitory Concentration (MIC) and minimum bactericidal concentration (MBC), the technique of microdilution and by counting Colony Forming Units (CFU) using concentrations of 250, 500 , 750 and 1000 μg/ml of EPO and concentrations of 100 and 1000 μg/ml dilapiol. The bacterial inoculum used was adjusted to 1x104 range from 0.5 Mc Farland. As a negative control inoculum was used along with Tween 20, solubilising the essential oil and dilapiol, and as positive control we used the chloramphenicol 0.05 mg/mL. These compounds were tested against strains of S. aureus ATCC 25923, S. epidermidis ATCC 12228, MRSA hospital, and S.epidermidis, S. lentus multiresistant nosocomial. The results showed that the EPO showed the MIC 90% of 500 μg/mL and MBC of 1000 μg/mL against S. aureus ATCC 25923, whose concentration of 500 μg/mL was able to inhibit 60% of bacterial growth. The strain MRSA, the EPO showed a small inhibition (10%) at a concentration of 750 μg/mL, being obtained in the MIC90% 1000 μg/mL. In S. epidermidis ATCC 12228, the OEPA showed antimicrobial activity with MIC90% at 500 μg/mL and MBC at 750 μg/mL. For the strain S. epidermidis multiresistant, the PEO was able to inhibit only 35% growth of this strain at a concentration of 750 μg/mL, but the value obtained in the MIC90% 1000 mg/mL. As for dilapiol, the compound showed antimicrobial activity against a strain of S. aureus ATCC 25923 at a concentration of 1000 μg/mL, 100% growth inhibiting (CBM). On the other hand, had no antimicrobial effect on MRSA strain nor S. lentus multiresistant. Furthermore, the dilapiol inhibited only 20% of the growth of S. epidermidis ATCC 12228 and S. epidermidis multiresistant concentration of 1000 μg/mL. Thus, the data show a moderate antibacterial activity of the essential oil, and the dilapiol showed weak in vitro antimicrobial activity.
Acesso aberto (Open Access)
Avaliação da atividade antiplasmódica in vitro dos óleos de Andiroba (Carapa guianensis Aubl.) e Pimenta-de-macaco (Piper aduncum L)
(Universidade Federal do Pará, 2010) MIRANDA JUNIOR, Raimundo Nonato Cardoso; DOLABELA, Maria Fâni; http://lattes.cnpq.br/0458080121943649; MAIA, José Guilherme Soares; http://lattes.cnpq.br/1034534634988402
In search of new antimalarial drugs, two typical species of the Amazon region and a fraction rich limonoids were the object of this study: Carapa guianensis Aubl. (Meliaceae), known popularly as andiroba traditionally used as an insecticide and fighting malaria, the species Piper aduncum L. (Piperaceae), known popularly as the pimento-de-macaco, used to treat inflammatory diseases and the fraction rich limonoids obtained from Carapa guianensis. Crude oil and fraction were tested in vitro using methods described by Rieckman and col. (1980) modified by Carvalho (1990) with Plasmodium falciparum clones W2 and Dd2. These studies showed that the oils had antiplasmodial activity, with a concentration of 0.82ng/mL and 8.2mg/mL andiroba oil showed an inhibition he W2 clone was 100% and Dd2 to 71% (IC50 9.4 μg/ml) after 72h of exposure respectively. For the fraction at a concentration of 3.1mg/mL, clone W2, was 100% and Dd2 to 82% (IC50 0.4 μg/ml), after 72h of exposure. The pimento-de-macaco oil overalls had a concentration of 1.30ng/mL for the W2 clone inhibition of 100% and the Dd2 to 77% after 72h of exposure to a concentration of 10.3mg/mL. The results with the chili oil overalls at a concentration of 1.30ng/ml the inhibition was 100% in clone W2 and Dd2 clone at a concentration of 10.3mg/mL, inhibition was 77% after 72h of exposure.
Acesso aberto (Open Access)
Avaliação toxicológica do óleo essencial de Piper aduncum L.
(2008-06) SOUSA, Pergentino José da Cunha; BARROS, Carlos Augusto Lima; ROCHA, José Carlos da Silva; LIRA, Denisléia Sertão; MONTEIRO, Gisele Muniz; MAIA, José Guilherme Soares
The aim of this work was the acute and subacute toxicological evaluation of the essential oil of Piper aduncum with the determination of the LD50 in mice and the analysis of their hematological and biochemical parameters in rats. The plant is used in the Amazon folk medicine for several diseases and the phenylpropanoid dilapiolle is the main constituent of its essential oil, possessing insecticidal, fungicidal, bactericidal, larvicidal and molluscicidal properties. The LD50 was 2.400 ± 191.7 mg/kg. The essential oil did not change the hematological and biochemical parameters in a significant manner when compared with the control in the subacute treatment, excepting the reduction of creatinine. The LD50 and the hematological and biochemical results have suggested that the essential oil presents low toxicity.
Acesso aberto (Open Access)
Collecting and evaluation of germplasm of spiked pepper from Brazilian Amazon
(2010-06) GAIA, José Maria Demetrio; MOTA, Milton Guilherme da Costa; CONCEIÇÃO, Carmen Célia Costa da; MAIA, José Guilherme Soares
Spiked pepper (Piper aduncum L.) is an aromatic plant species with high essential oil production. It is a species that occurs abundantly in the Brazilian Amazon. Its essential oil has exploitable biological properties in the human health and agriculture. Aiming to study its germplasm toward future use in genetic breeding programs, collecting was carried out (inflorescences, cuttings, leaves and thin branches) in ten provenances from the Brazilian Amazon. Twelve morphoagronomic traits were determined to take the data: number of leaves by branch, length of leaf, width of the leaf, circumference of the older branch, height of the plant, number of orthotropic branches, number of plageotropic branches, length of the internodes, number of spikes per branches, yielding of oil, content and production of dillapiole, as well as data on the environment and populations of spiked pepper. The inflorescences and cuttings were encoded and sent for the Federal Rural University from Pará State (UFRA), Brazil, for propagation. The leaves and thin branches were sent for the Emílio Goeldi Museum, from Pará State (MPEG) for extraction of essential oil (hydrodistillation). Estimators of amplitude of variation, mean, standard deviation and coefficient of variation were utilized to study the phenotypical variability. The morphoagronomic traits of largest variability were number of orthotropic branches, number of spikes per branch, circumference of the older branch and the content and production of dillapiole. This species has adapted to many different environments of vegetation, soil, climate, relief and drainage becomming easy the domestication and cropping. There is morphoagronomic variability pleasing the selection and genetic breeding.
Acesso aberto (Open Access)
Comportamento higroscópico de partes aéreas de pimenta-de-macaco (Piper aduncum L.)
(2015-04) SILVA, Carolina de Lourdes Oliveira Cruz e; FARIA, Lênio José Guerreiro de; COSTA, Cristiane Maria Leal
Desorption isotherms of monkey pepper were determined by static gravimetric method at temperatures 35, 45, and 55 ºC, over a relative humidity range of 5.5-81%. Three mathematical models were applied to analyse the experimental data. The modified GAB model showed the best adjustment of the experimental data. The isosteric heat and differential entropy were determined by applying Clausius-Clapeyron and Gibbs-Helmholtz equations, respectively. The isosteric heat and the entropy of desorption isotherm presented similar behavior. The enthalpy-entropy compensation theory was applied to the isotherms, indicating that the aerial parts desorption mechanism are enthalpy-controlled.
Acesso aberto (Open Access)
Morphologic characterization of spiked pepper's germplasm
(2011-06) GAIA, José Maria Demetrio; MOTA, Milton Guilherme da Costa; CONCEIÇÃO, Carmen Célia Costa da; MAIA, José Guilherme Soares
Spiked pepper is a plant species with properties that allow the development of natural agrochemicals and medicines, showing large potential of use by humanity. With aim to ascertain the phenotypical variability, 41 parentals were analyzed, sampled in the States of Pará and Amazonas. Principal Component analysis and Jolliffe's criterion were utilized for discarding of variables, subsidized by the Pearson's Correlation. It took seven components to explain 80% of the variation. The essential oil yield and number of leaves per branch were suggested to be discarded because they are the characteristics that have contributed least to the total variance. The 3D scatter diagram constituted a relatively homogeneous and continuous clustering, identifing a divergent pair: PA-020 (Marabá-PA) and PA-035 (Santa Isabel-PA). The analyzed traits have variability potentially able to discriminate the parentals, whereas 83.3% of such traits can be used for this purpose. The divergent genotypes identified on 3D scatter diagram analysis can be used in breeding programs for the development of superior genotypes. A comparison with preexisting molecular data of some genotypes permited to conclude that there was one certain agreement degree between morphological and molecular characterizations and that molecular characterization presented higher discriminatory power, using a smaller number of genotypes, identifying dissimilar genotypes and clusters, although analyzed by different multivariate statistic methods.