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Navegando por Assunto "Plantas aromáticas"

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    Antioxidant capacity and larvicidal activity of essential oil and extracts from Lippia grandis
    (2011-02) DAMASCENO, Evelyn Ivana Trindade; SILVA, Joyce Kelly do Rosário da; ANDRADE, Eloisa Helena de Aguiar; SOUSA, Pergentino José da Cunha; MAIA, José Guilherme Soares
    The leaves and thin branches of Lippia grandis Schauer, Verbenaceae, are used for flavoring of food in the Brazilian Amazon, as substitute for oregano. In this study the constituents of the essential oil were identified and the antioxidant capacity and larvicidal activity of the oil and methanol extract and its sub-fractions were evaluated. A sensory evaluation was determined in view of absence of toxicity. The oil showed a yield of 2.1% and its main constituents were thymol (45.8%), p-cymene (14.3%), γ-terpinene (10.5%), carvacrol (9.9%) and thymol methyl ether (4.8%), totalizing 85%. The DPPH radical scavenging activity showed values for the EC50 between 9.0 and 130.5 µg mL-1 and the TEAC/ABTS values varied from 131.1 to 336.0 mg TE/g, indicating significant antioxidant activity for the plant. The total phenolic content ranged from 223.0 to 761.4 mg GAE/g, contributing to the antioxidant activity observed. The crude extracts inhibited the bleaching of β-carotene and the oil showed the greatest inhibition (42.5%). The oil (LgO, 7.6±2.4 µg mL-1) showed strong larvicidal activity against the brine shrimp bioassay. The sensory evaluation was highly satisfactory in comparison to oregano. The results are very promising for the use of L. grandis in seasoning and antioxidant products.
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    Avaliação da atividade antinociceptiva e antiinflamatória do óleo essencial de Hyptis crenata (Pohl) ex Benth
    (Universidade Federal do Pará, 2008-11-07) BRAVIM, Luciana Silva; MAIA, José Guilherme Soares; http://lattes.cnpq.br/1034534634988402; SOUSA, Pergentino José da Cunha; http://lattes.cnpq.br/9909053957915090
    Hyptis crenata (Pohl) ex Benth. is a herbaceous aromatic and medicinal plant, belongs to Lamiaceae, popularly known as salva-do-marajó, malva-do-marajó and hortelã-bravo. This plant is distributed since the mouth of Amazonas River, Marajó Island, Pantanal, and extending up to the states of Minas Gerais. Its essential oil is characterized by presence of monoterpenes and sesquiterpenes. The leaf tea is used as sudorific, tonic, stimulant, as well as to treat the eyes and throat inflammation, constipation and arthritis. Based in these information, we decided to evaluate the antinociceptive and anti-inflammatory activity of the oil (HcEO) of Hyptis crenata in mouse throught the following tests: abdominal constriction, hot plate, formalin, croton oil-induced ear edema, rat paw edema induced by dextran and carrageenan, carrageenan-induced peritonitis. The statistical method used in this study was ANOVA followed by multiple comparison (Student-Newman-Keuls test or Student t test). The essential oil was extracted by hydrodistillation, yield was 0,6% and their main components were monoterpenes (94,5%). The lethal dose (DL50) was 5000 mg/kg. In the abdominal constriction test, at doses of 250, 350 and 500 mg/kg, the HcEO inhibited the abdominal constrictions in 22,56%, 60,76% and 75,53%, respectively, in a dose-dependent manner when compared to the control group. The DE50 calculated was 364,22 mg/kg with a correlation coefficient of 0,9341. The oil not changed significantly the time of latency in the hot plate test. HcEO (364,22 mg/kg) caused an inhibition of the phase I in 26,42% and an inhibition of the phase II in 43,86% of the formalin test. Naloxone, an antagonist of opioid receptor, reverted the analgesic effect of EOHc. HcEO reduced croton oil-induced ear edema in 44,26%. In rat paw edema induced by dextran, HcEO produced inhibition at the dose of 364,22 mg/kg, but the rat paw edema induced by carrageenan this inhibition was not observed. In carrageenan-induced peritonitis, HcEo significantly decreased the leucocyte and neutrophil migration in 47,55% e 66,47%, respectively. Based on the results we are suggesting that the essential oil of H. crenata has an antinociceptive activity.It is probably as consequence of direct action on the nociceptive fiber, as well as the opioid system is involved in this effect. Further, our results suggest that anti-inflammatory activity of HcEO is probably of peripheral origin. It can be suggested, too, that the possible components responsible for these actions are the compounds monoterpenes presents in HcEO.
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    Database of the Amazon aromatic plants and their essential oils
    (2009) MAIA, José Guilherme Soares; ANDRADE, Eloisa Helena de Aguiar
    The aromatic flora of the Amazon has been inventoried for 30 years. In this sense, were made over 500 field trips to collect over 2500 plants and to obtain more than 2000 essential oils and aroma concentrates, all of them submitted to GC and GC-MS. This work led to the creation of a database for the aromatic plants of the Amazon, which catalogs general information about 1250 specimens. The database has allowed the publication of the chemical composition of the oils and aromas of more than 350 species, associated with a larger number of chemical types. The essential oils of many species offer optimum conditions for economic exploitation and use in national and international market of fragrances, cosmetics, agricultural and household pesticides.
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    Estudo do crescimento bacteriano na presença de óleos essenciais de Dysphania ambrosioides l. e Ocimum campechianum mill. para avaliar seus potenciais como antissépticos bucais
    (Universidade Federal do Pará, 2015-08-31) MOURA, Luiziana Barbosa; SANTOS, Alberdan Silva; http://lattes.cnpq.br/5976702134131016
    The aromatic plants like Dysphania ambrosioides (mastruz) and Ocimum campechianum (Alfavaca), that are part of folk medicine in Brazil, have phenylpropanoids and terpenoids compounds in their essential oils as result of secondary metabolism that influence the adaptation and defense of these species in environment; particularly, defense against microrganisms. The objective of this study was to evaluate the antimicrobial activity of essential oils of D. ambrosioides and O. campechianum against pathogenic bacteria mouth. For this they used the disk diffusion method on agar and broth microdilution adapted. The plants were obtained in Santa Izabel do Pará, their leaves were washed and weighed; the essential oil was obtained by hydrodistillation. After, the components of essential oils were identified by gas chromatography-mass spectrometry. Bacteria strains used were: Lactobacillus casei (ATCC 7469), Lactobacillus fermentum (ATCC 9338), Streptococcus mutans (ATCC 25175), Streptococcus oralis (ATCC 10557) and Agregatibacter actinomycetencomitans (ATCC 29522). Bacterial suspensions were prepared for testing and 0.5 McFarland standard. As a positive control we adopted the digluconate of chlorhexidine 0.12%. Inhibition halos were observed for all samples in different concentrations of each essential oil (1%, 5%, 10%, 25%, 50% and 75%). The largest halos were found to A. actinomycetencomitans. In the microdilution test the basil oil inhibited the bacteria S. mutans, the main etiological factor for caries in concentration of 1%; mastruz the oil inhibited L. casei, microorganism that enhances the process of decay in concentrations from 10%; Both oils inhibited the growth of A. actinomycetemcomitans, and can be effective against periodontal disease caused by that pathogen. Plant species of this study produce secondary metabolites classes with potential applications in the development of dental products.
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