Navegando por Assunto "Polimofismo de nucleotídeo"

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Acesso aberto (Open Access)
Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic
(Universidade Federal do Pará, 2015-06) LIMA, Eleonidas Moura; LOPES, Otávio Sérgio; SOARES, Leonardo Ferreira; ARRUDA, Talitta Dantas; GIGEK, Carolina Oliveira; MELO, Cynthia Germoglio Farias; SMITH, Marília de Arruda Cardoso; OLIVEIRA, João Ricardo Gonçalves; MEDEIROS, Arnaldo Correia de; DELATORRE, Plínio; BURBANO, Rommel Mario Rodriguéz
Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ 2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ 2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination.
Acesso aberto (Open Access)
Toll-like receptor 3 gene polymorphisms are not associated with the risk of hepatitis B and hepatitis C virus infection
(Universidade Federal do Pará, 2015-04) SÁ, Keyla Santos Guedes de; PIRES NETO, Orlando de Souza; SANTANA, Bárbara Brasil; GOMES, Samara Tatielle Monteiro; AMORAS, Ednelza da Silva Graça; CONDE, Simone Regina Souza da Silva; DEMACHKI, Sâmia; AZEVEDO, Vânia Nakauth; MACHADO, Luiz Fernando Almeida; FEITOSA, Rosimar Neris Martins; ISHAK, Marluísa de Oliveira Guimarães; ISHAK, Ricardo; VALLINOTO, Antonio Carlos Rosário
Introduction: The present study investigated the prevalence of two single-nucleotide polymorphisms (SNPs) in the Toll-like receptor 3 (TLR3) gene in patients infected with hepatitis B virus (HBV) and hepatitis C virus (HCV). Methods: Samples collected from HCV (n = 74) and HBV (n = 35) carriers were subjected to quantitative real-time PCR (qPCR) to detect the presence of the SNPs rs5743305 and rs3775291 in TLR3 and to measure the following biomarkers: alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), and prothrombin time (PT). A healthy control group was investigated and consisted of 299 HCV- and HBV-seronegative individuals. Results: No signifi cant differences in allele, genotype and haplotype frequencies were observed between the investigated groups, and no association was observed between the polymorphisms and histopathological results. Nevertheless, genotypes TA/AA (rs5743305) and GG (rs3775291) appear to be associated with higher levels of ALT (p<0.01), AST (p<0.05) and PT (p<0.05). In addition, genotypes TT (rs5743305; p<0.05) and GG (rs3775291; p<0.05) were associated with higher GGT levels. Conclusions: This genetic analysis revealed the absence of an association between the polymorphisms investigated and susceptibility to HBV and HCV infection; however, these polymorphisms might be associated with a greater degree of biliary damage during the course of HCV infection.