Navegando por Assunto "Principal component analysis"

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Acesso aberto (Open Access)
Arranjos produtivos locais de agricultura temporária no estado do Pará: uma aplicação do modelo de análise de componentes principais
(Universidade Federal do Pará, 2017) CARVALHO, Abner Vilhena de; CARVALHO, André Cutrim; CARVALHO, David Ferreira; FILGUEIRAS, Gisalda Carvalho; ARAÚJO, Ana Cláudia de Sousa; SOARES, André Araújo Sombra
The objective of this article is to discuss the Local Productive Arrangements of temporary agriculture in the State of Pará, from the analysis of the cluster process, since they present a great potential for the formation of productive chains. The methodological procedure consists of the grouping of productive activities belonging to the same class, whose actions are completed in the production chain horizontally or vertically, which makes the model constructed through the analysis of principal components more robust. The main conclusion is that temporary crops are able to form the basis of potential APLs in Pará, even though the production of temporary crop products is still provided by small and medium producers and sold to intermediaries or directly to informal networks of the region. Therefore, it is necessary to establish a rural development policy aimed at production based on the rational cultivation of temporary crops capable of attracting rural agroindustries to the formation of productive chains.
Acesso aberto (Open Access)
Estudo do mecanismo conformacional da proteína 3-hidroxi-3- metilglutaril Coenzima A Redutase (HMGR) com as estatinas e substrato através de Dinâmica Molecular, PCA e Energia Livre
(Universidade Federal do Pará, 2017-08-03) COSTA, Clauber Henrique Souza da; SILVA, Jerônimo Lameira; http://lattes.cnpq.br/7711489635465954; https://orcid.org/0000-0001-7270-1517
Cholesterol is a substance of paramount importance for all animals. However, its high level in the human body is linked to the two major diseases that kill the world: ischemic heart disease and stroke. One of the synthetic drugs used in the treatment of hypercholesterolemia are statins, inhibitors of 3-hydroxy-3-methylglutaryl Cozyme A reductase (HMGR), which act primarily on the liver by inhibiting a conversion of the HMG-CoA substrate into mevalonic acid, which is the metabolite Cholesterol precursor. Studies Molecular Dynamics (MD) combined with Principal Component Analysis (PCA) were performed to verify the mechanism of the changes in the Cterminal Flap domain form (residues His861, Leu862, Val863, Lys864, Ser865 and Hys866) after binding substrate and efficient statins in inhibiting the HMGR enzyme. A total of 500 ns of MD simulation time were performed in this study. Binding Free Energies calculations were used, which estimate that the structural mechanism of the Flap is related to an action of the HMGR protein, since domain control or access to the active site of the enzyme. The results also show that the structural modification of Flap increases the energy contribution of the system by involving larger interactions with catalytic residues and, consequently, an ability to inhibit cholesterol production, as observed for the catalytic His866, which has a very favorable contribution when the Flap is in the closed state, with energy of -14,802 Kcal/mol, and when the Flap passes to the open state the contribution is less favorable, with -1,022 Kcal/mol, for 1 inhibitor, showing that in the closed state the catalytic residue is directly involved and contributes in a favorable way to the system, leading to a better understanding of the conformational changes of HMGR after a binding of statin derivatives and HMG-CoA substrate.