Navegando por Assunto "Pseudomonas aeruginosa"

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Acesso aberto (Open Access)
Aspectos clínicos e epidemiológicos da infecção por pseudomonas aeruginosa em pacientes portadores de fibrose cística no Estado do Pará
(Universidade Federal do Pará, 2003-12-23) MARTINS, Valéria de Carvalho; VIEIRA, José Luiz Fernandes; http://lattes.cnpq.br/2739079559531098
Pseudomonas aeruginosa is a gram-negative motile rod; they are important pathogens in neutropenic patients, burn and artificially ventilated patients in Intensive Care Units, where they may cause nosocomial infections. At this condition, the infection seems to be serious and sometimes lethal. However, in Cystic Fibrosis patients the course of infection by P. aeruginosa, has become severe chronic lung infection, because this bacteria produce many toxins and others virulence factors with potential effect on the lungs of this patients that are thought to play a role during establishment of the initial persistent colonization of the Cystic Fibrosis respiratory tract. The most characteristic feature of persistent P. aeruginosa infection is the production of mucoid alginate and the formation of the micro colonies in the lungs of the patients that is the survival strategy of environrnent bacteria. P. aeruginosa growing in alginate biofilms is highly resistant to antibiotics and is usually associated with progressive loss of pulmonary function. This research wants to study Cystic Fibrosis patients chronically colonized by P. aeruginosa who has been taken care on University Hospital João de Barros Barreto, Belém (Pará), in 2003 year, at about epidemiological and clinicalaspects. The sputum of these patients, have been collected by expectoration and swab of throat, to microbiological study realized on microbiology laboratory of this hospital. Thirty two patients with Cystic Fibrosis have been evaluated and divided according microbiological aspect of their sputum in three groups: patients who have never been infected by P. aeruginosa (Gl), patients that have been infected by this bacterial but not colonized (G2), patients chronically colonized by this microorganism. Patients who concern to G3 group have been more serious respiratory complications than patients that belong to the others groups. The pattern mucoid of P. aeruginosa has been significant on patients' sputum of G3. The incident of non- mucoid strains in most patients of groups G2 and G3 seems to be similar. Respiratory symptoms have been important during diagnosis of Cystic Fibrosis. The median age for fibrosis cystic diagnosis was about 7,69 years. Certainly several events contribute to support P. aeruginosa respiratory colonization on Cystic Fibrosis patients of G3, like: low social and economic conditions, lateness diagnosis, malnutrition and pattern Cystic Fibrosis gene mutation.
Acesso aberto (Open Access)
Função pulmonar dos pacientes com fibrose cística colonizados por Pseudomonas aeruginosa no Pará
(Universidade Federal do Pará, 2008) FALCÃO, Edilene do Socorro Nascimento; CARNEIRO, Irna Carla do Rosário Souza; http://lattes.cnpq.br/4389330944043163; SOUSA, Rita Catarina Medeiros; http://lattes.cnpq.br/3560941703812539
Pseudomonas aeruginosa is an opportunistic pathogen in cystic fibrosis becoming the most frequent cause of lung infection; it is responsible for high mortality rates in patients who present this disease. The goal of this study is to showing spirometric and clinic characteristics of patients who present cystic fibrosis and who are attended at João de Barros Barreto University Hospital (HUJBB), in the State of Pará. A retrospective study was performed with 44 patients´ notes record who were attended at HUJBB during the 1997 to 2007 time frame; these patients fit into inclusion criteria, in which 14 presented P. aeruginosa bacteria colony, nine patients died. The significance rate presented was of 5%. Within the group who was colonized by P. aeruginosa, ten patients were women and the median age for their initial symptoms was of 0.3 ± 0.6 year which was significantly different when compared with patients who didn´t present the bacteria colony (p<0.05). The median age for diagnosis was of 13.1 ± 10.8 in colonized patients and all of them presented respiratory symptoms pertaining to the diagnosis. The median of the predicted percentage values of spirometries for initial and final evaluation of the colonized group was VEF1 (60.0 ± 25.0%) and (47,82 ± 16.1%) and FEF25-75% (42.5 ± 22.9%) and (26.5 ± 17.9%), and in the non-colonized group, the median was VEF1 (79.2 ± 21.0%) and (79,6 ± 18.0%) and FEF25-75% (69.2 ± 26.7%) and (68.9 ± 26.8%), respectively (p<0.005). The median initial Shwachman score in the colonized patients was 42.9 ± 13.5 and in the non-colonized patients it was 68.4 ± 15.1 (p<0.0001), and at the final evaluation the median was 36.6 ± 18.7 and 73.6 ± 12.3 (p<0.0001), respectively, showing important difference between the two groups. Factors related to deaths found in the study were related to P. aeruginosa colonization, inadequate nutritional status and reduced VEF1. In the studied casuistry, a larger damage to respiratory function and older median age for diagnosis were found in the State of Pará when compared to other Brazilian States. These data reinforce the need for action towards precocious diagnosis in the State of Pará so that an efficient and guided therapeutic approach is put into practice guiding to survival increase and quality of life improvement for these individuals, which is the main goal of professionals involved in the assistance practice area.
Acesso aberto (Open Access)
Influência da velocidade de circulação do leite na adesão de Pseudomonas aeruginosa sobre aço inoxidável
(2009-09) FIGUEIREDO, Hamilton Mendes de; ANDRADE, Nélio José de; OZELA, Eliana Ferreira; MORALES, Gundisalvo Piratoba
The influence of the flow milk circulation in the bacterial adhesion of Pseudomonas aeruginosa was evaluated by simulation tests through a circuit model of milk processing. The circuit is composed of a tubulation of stainless steel AISI 304, with 1.9 cm of diameter, 5.8 m of length and a tank of 25 L used as the reservoir of the product and sanitizer solutions. The reservoir was coupled to a centrifugal bomb of ½ HP to impel the food or sanitizer solutions for the system equipped with 90º and T cylindrical stainless steel specimens. The speed of circulation values were 0.5, 1.0 and 1.5 m.s–1, corresponding to turbulent flow with number of Reynolds 14.000, 28.000 and 42.000, respectively. When flow of 0.5 m.s–1 was used 10.7% the cells remained adhered, however at the speed values of 1.0 and 1.5 m.s–1 the adhesion percentages were 5.36 and 4.9%, respectively. These findings indicate a lower removal rate of adhered cells as flow decreases allowing higher number of bacteria to adhere to the production line, which can favor the biofilm formation.
Acesso aberto (Open Access)
Perfil epidemiológico, letalidade e caracterização molecular de Pseudomonas aeruginosa multirresistentes, produtoras de metalobetalactamases de isolados clínicos em Unidade de Terapia Intensiva na região Norte
(Universidade Federal do Pará, 2014) MATOS, Eliseth Costa Oliveira de; CARNEIRO, Irna Carla do Rosário Souza; http://lattes.cnpq.br/4389330944043163; LIMA, Karla Valéria Batista; http://lattes.cnpq.br/9795461154139260
The bacterial resistance is responsible for major concerns about the great problems on health systems. The proposal of this study was to determine the epidemiological, lethality and molecular characteristics of Pseudomonas aeruginosa isolated from patients admitted to the Intensive Care Unit (ICU) in a teaching hospital in city of Belem, Pará, Brazil and analyze the performance of susceptibility testing for P. aeruginosa in automated VITEK -2 to study the antimicrobial resistance. The target people of this study consisted of 54 patients with P. aeruginosa infection at the adult , pediatric and neonatal ICUs from January 2010 to March 2012. For the epidemiological research, demographic data, comorbidities, length of stay were collected. Episodes of Healthcare-associated infections were defined in accordance to Center for Disease Control and Prevention (CDC), use of invasive procedures, previous therapy and patient outcome, Jackson MacCabe score, APACHE II score and Charlson score were documented. The bacterial identification was performed by biochemical tests and VITEK-2 system. The sensitivity test was performed with the application of the method of broth microdilution , and detection of metallo-beta-lactamase by the double disc and PCR method. The test of Genotyping was made by amplification throug DiversiLab system. The P. aeruginosa was the second most common pathogen in the hospital and this occurrence was higher in the adult ICU. The respiratory infection was predominant in the adult ICU, while bloodstream infection was prevalent in pediatric and neonatal ICU. The multidrug resistance was higher in the adult ICU, the average age of patients was 28 years, with an average length of stay of 87.1 days, the diseases were potentially fatal and major risk factors for acquisition of PaMR infection were the use of mechanical ventilation and urinary catheterization. Through the meta-analysis study, we found that infections caused by MR strains showed higher mortality rate, and were twice as likely to death occurs, multidrug resistance a factor of poor prognosis in patients with infection PaMR. Four isolates producers blaSPM-1 variant was detected and genotyping figured the presence of genetic similarity greater than 97%, the changes observed may be related to selective pressure existing in the adult ICU, coupled with inadequate therapy. This study demonstrated the importance of investigations of infections with multidrug-resistant pathogens in critical care units, contributing to these data the possibility of better monitoring and control of these infections.