Navegando por Assunto "Rats"

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Acesso aberto (Open Access)
Effect of andiroba oil on periodontitis in Wistar rats
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-06) CARMONA, Glaucia Babeto; TEIXEIRA, Renan Kleber Costa; BRITO, Marcus Vinicius Henriques; PONTES, Flávia Sirotheau Corrêa; ANDRADE, Eloisa Helena de Aguiar; FONSECA, Felipe Paiva; COSTA, Ricardo Miranda Brito; CARVALHO, Francideise Martins
PURPOSE: To evaluate the effects of andiroba oil on the periodontitis in rats. METHODS: The periodontitis was induced by the placement of cotton ligatures around the cervix of the second upper molars on fifteen rats, and waiting fifty days. The animals were randomly distributed into three groups: saline group, andiroba oil group and meloxican group, differentiated by substance used in the treatment of periodontitis. The groups received the respective substance by gavage for seven days, after the periodontitis induced. It was analyzed the score of inflammatory cells and the measurement from the cemento-enamel junction to the bone crest. RESULTS: The andiroba oil group (p=0.008) and meloxican group (p=0.0347) show a less score of inflammatory cells than saline group, however there weren't difference between them (p=0.2754). Regarding the analysis of measurement from the cemento-enamel junction to the bone crest, there was no difference between groups studied (p=0.3451). CONCLUSION: Andiroba oil decreased the quantity of inflammatory cells, however, it didn't have an effect on the measurement of alveolar bone loss, like the treatment with Meloxican®.
Acesso aberto (Open Access)
Effects of hypertonic saline solution associated to remote ischemic perconditioning in kidney ischemia/reperfusion injury in rats
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2017-03) BRITO, Marcus Vinicius Henriques; YASOJIMA, Edson Yuzur; PÉRCARIO, Sandro; RIBEIRO JUNIOR, Rubens Fernando Gonçalves; CAVALCANTE, Lainy Carollyne da Costa; MONTEIRO, Andrew Moraes; COUTEIRO, Rodrigo Paracampo; RODRIGUES, Ivone Aline da Silva; SANTOS, Hellen Aparecida Geyer dos
To evaluate the effects of hypertonic saline solution associated to remote ischemic perconditioning in renal ischemia/reperfusion injury in rats. Methods: Twenty five male rats (Wistar) underwent right nephrectomy and were distributed into five groups: Sham group (S); Ischemia/Reperfusion group (I/R) with 30 minutes of renal ischemia; Remote ischemic perconditioning group (Per) with three cycles of 10 minutes of I/R performed during kidney ischemia; Hypertonic saline solution group (HSS) treated with hypertonic saline solution (4ml/kg); remote ischemic perconditioning + Hypertonic saline solution group (Per+HSS) with both treatments. After reperfusion, blood samples were collected for BUN and creatinine serum levels analyzes. TBARS were evaluated in plasma and renal tissue to assess oxidative stress. Kidney histopathological examination were performed. Results: Per+HSS group showed a lower degree of renal dysfunction in relation to I/R group, whereas the technique of remote ischemic perconditioning isolated or associated with saline solution significantly reduced oxidative stress and histological damage. Conclusion:Remote ischemic perconditioning associated or not to saline solution promoted reduction of acute renal injury induced by ischemia/reperfusion.
Acesso aberto (Open Access)
Immunohistochemistry of the uterine cervix of rats bearing the Walker 256 tumor treated with copaiba balsam
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-03) BOTELHO, Nara Macedo; CORRÊA, Suelen Costa; LOBATO, Rodolfo Costa; TEIXEIRA, Renan Kleber Costa; QUARESMA, Juarez Antônio Simões
PURPOSE: To investigate the immunohistochemistry of the uterine cervix of 20 Wistar rats (Rattus norvegicus) bearing the Walker 256 tumor, treated with copaiba oil (Copaifera officinalis). METHODS: The animals were grouped into four subgroups, with five rats each: the GCT and GCopT received distilled water and topically copaiba, respectively, while the GCG and GCopG received distilled water and copaiba by gavage, respectively. The substances were administered for nine days. On the 12th day, after euthanasia, the tumor pieces were sent to the identification of T CD4+, T CD8+ and Natural Killer cells. RESULTS: It was found that the pattern of expression for specific markers of phenotypes of cells involved in tumor immune response was similar in all groups, regardless the administration way of copaiba oil (topical or gavage). CONCLUSION: Copaiba balsam, administered either topically or by gavage, did not alter the pattern of tumor immune response in rats bearing Walker 256 Tumor.
Acesso aberto (Open Access)
Influence of oophorectomy on glycemia and lipidogram
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2018-05) TEIXEIRA, Renan Kleber Costa; FEIJÓ, Daniel Haber; VALENTE, André Lopes; CARVALHO, Luan Teles Ferreira de; GRANHEN, Hícaro Donato; PETROIANU, Andy; BOTELHO, Nara Macedo
Methods:Fifty six female rats were divided into the following 7 groups (n = 8): group 1 - sham group, group 2 - oophorectomy 30 days, group 3 - oophorectomy 35 days, group 4 - oophorectomy 40 days, group 5 - oophorectomy 45 days, group 6 - oophorectomy 70 days, group 7 - oophorectomy 55 days. Animals were following by number of days according the group. Was evaluated the serum levels of glucose and lipid profile. Results: The oophorectomized rats presented higher glycemia. Groups 3, 4, 6 and 7 had a higher glycemia and LDL levels (except for group 6) and groups 6 and 7 had lowest levels of HDL. Group 7 had highest level of VLDL than oophorectomy groups. There was no difference in triglycerides levels. Conclusion: Oophorectomy was related to dyslipidemia and insulin resistance, mainly after 50th days.
Acesso aberto (Open Access)
Influence of tramadol on functional recovery of acute spinal cord injury in rats
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2018-12) CHAVES, Rosa Helena de Figueiredo; SOUZA, Celice Cordeiro de; FURLANETO, Ismari Perini; TEIXEIRA, Renan Kleber Costa; OLIVEIRA, Carolina Pinheiro de; RODRIGUES, Emanuelle de Matos; SANTOS, Daniel Arthur Santos dos; SILVA, Renata Cunha; PENHA, Nelson Elias Abrahão da; LIMA, Ana Rita de
Purpose: To evaluate the influence tramadol on functional recovery of acute spinal cord injury in rats. Methods: Ten rats were divided into two groups (n = 5). All animals were submitted by a aminectomy and spinal cord injury at eighth thoracic vertebra. In control group, the rats didn’t receive any analgesic. In tramadol group, the rats received tramadol 4mg/Kg at 12/12h until 5 days by subcutaneous. Animals were following by fourteen days. Was evaluated the Basso, Beattie, Bresnahan scale (locomotor evaluation) and Rat Grimace Scale (pain evaluation) at four periods. Results: There no difference between the groups in locomotor evaluation in all periods evaluated (p>0.05) and in both groups there was a partial recover of function. The tramadol group show a lower pain levels at the first, third and seventh postoperatively days when comparing to the control group. Conclusion: The tramadol as an analgesic agent don’t influence on functional recovery of acute spinal cord injury in rats.
Acesso aberto (Open Access)
Lesão intestinal após isquemia-reperfusão: estudo comparativo usando sal tetrazólico (MTT) e histologia
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2001-03) BRITO, Marcus Vinicius Henriques; ARAÚJO, Marialva Tereza Ferreira de; ACÁCIO, Glayce June Sasaki; ACÁCIO, Glayce Josy Sasaki; REIS, José Maciel Caldas dos
Many methods are used to evaluate ischemia reperfusion injury, but the most employed one is the histological study. However, it only demonstrates on mucosal tunic, the index of lesion and morphologic preserved cells, but not the index of viable functional cells, present in the sample. With this purpose, a colorimetric method was used, employing Methyl Thiazolyl Blue (MTT). The experiment was conducted in 30 Wistar male rats, distributed in 3 groups: Group Control (GC), Group ischemia and reperfusion-1 (GIR-1) and Group ischemia and reperfusion-3 (GIR-3), with 10 animals each. The Groups GIR-1 and GIR-3 were submitted to intestinal ischemia for 30 minutes by a false ligature of superior mesenteric artery, and submitted to euthanasia after 1 and 3 days of reperfusion, when material was picked for absorbency and histological procedures. It was observed a smaller proportion of viable cells and a larger degree of mucosal lesion in GIR-3 group (p<0.05), while GC group was the one with the larger proportion of viable cells and smaller degree of the mucosal injury (p<0.05). This way we concluded that MTT is as effective and reliable as the histological study at evaluating alterations produced by intestinal ischemia-reperfusion.
Acesso aberto (Open Access)
New experimental model of exposure to environmental tobacco smoke
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2013-12) BRITO, Marcus Vinicius Henriques; YASOJIMA, Edson Yuzur; SILVEIRA, Edvaldo Lima; YAMAKI, Vitor Nagai; TEIXEIRA, Renan Kleber Costa; FEIJÓ, Daniel Haber; GONÇALVES, Thiago Barbosa
PURPOSE: To describe a new model to passive smoking for rodents. METHODS: Twenty rats were distributed into two study groups (N=10): control group (CG), that was not exposed to tobacco smoke and used as normal standard for biochemical and histological analysis; Experimental Group (EG), that Animals were exposed to the passive smoking; Euthanasia was performed after 14 days of exposure. The serum level of nicotine and histological analysis were performed. RESULTS: There was a statistical difference on the nicotine serum levels between Experimental and Control group, with level of 286 ±23 nanograma/mL in the EG and undetectable on CG (p<0.01). The histological study suggested the model efficacy producing alveolar destruction and emphysema in the EG compared with the insignificant lesions in the CG's lung. CONCLUSION: The model of exposure to environmental tobacco smoke for rodents induced easily the changes related to secondhand smoke.
Acesso aberto (Open Access)
Stem cells from adipose tissue improve the time of wound healing in rats
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2016-12) OHASHI, Camila Melo; CALDEIRA, Fabio Alves Morikawa; FEITOSA JUNIOR, Denilson José Silva; VALENTE, André Lopes; DUTRA, Paulo Roberto Witter; MIRANDA, Moysés dos Santos; SANTOS, Simone do Socorro Damasceno; BRITO, Marcus Vinicius Henriques; OHASHI, Otávio Mitio; YASOJIMA, Edson Yuzur
PURPOSE: To evaluate the Adipose Stem Cells (ACS) therapy efficacy on the time and quality of wound healing process in rats. METHODS: Nine male Wistar rats were randomly distributed into three groups I) 7 days of healing; II) 14 days of healing; III) 21 days of healing. Four incisions were made on the dorsal surface of each rat and then treated with intralesional ACS, meloxicam, and no treatment and ACS+meloxicam. Macroscopic evaluation was measured by percentage of healing and histopathological by hematoxylineosin was performed. RESULTS: All groups have the wound reduced during the three weeks (p<0.001) and after 14 days of healing had greater reduction than others. Wounds treated with ASC had accelerated healing in relation to no treatment and only meloxicam (p<0.001), excepting the ASC+Meloxicam that was similar (p=0.13). There was no difference in histopathological analysis between lesions. CONCLUSION: Adipose stem cell have benefits in reducing time of healing of experimental model of wound in rats, observed 7 days of after application.
Acesso aberto (Open Access)
Viabilidade celular da mucosa do intestino delgado de ratos, após correção de choque hipovolêmico com solução de NaCl 7,5%
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2003-08) BRITO, Marcus Vinicius Henriques; NIGRO, Amaury José Texeira; MONTERO, Edna Frasson de Souza; NASCIMENTO, José Luiz Martins do; SILVA, Paula Roberta Ferreira da; SIQUEIRA, Roberta Bianca Peres
Study the effect of the volemic correction with different solutions, in the mucous of the small bowel in rats. Were used 120 rats Wistar (Rattus norvegicus albinus), males, adults, seemingly healthy, with individual weight varying between 310 and 410g, originating from of the Instituto Evandro Chagas of Belém of Pará, submitted to an adaptation period of 15 days, receiving water and ration ad libitum, during the role experiment. For the research, ten animals were distributed, in groups and subgroups as following: Standard group (S), Shock group (Sh), Physiological Solution group (PS) and Hypertonic Solution Group (HS). The groups were divided in subgroups with 10 animals each, in agreement with the day of postoperative (DPO) foreseen for the euthanasis of the animals, (1st, 3rd or 7th DPO), being after this, picked material for cellular viability in every animals. RESULTS: The group PS took less quantity viable cells. The volemic correction with chloride of sodium solution at 7.5%, when compared the correction with chloride of sodium at 0.9% (isotonic solution), took the maintenance of larger amount of viable cells, in the small bowel in rats.