Navegando por Assunto "Sorologia"

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Acesso aberto (Open Access)
Avaliação da diversidade de insetos hematófagos da subordem Nematocera e de vertebrados silvestres: transmissão de arbovírus na área de influência do Projeto Salobo, Carajás, Pará
(Universidade Federal do Pará, 2009) MONTEIRO, Hamilton Antonio de Oliveira; VASCONCELOS, Pedro Fernando da Costa; http://lattes.cnpq.br/0973550817356564
Aiming to evaluate the diversity of hematophagous insects and sylvan vertebrates, as well as the arbovirus fauna present in the area, a longitudinal study was performed in the Salobo Project area, Mineral Province of Carajas, Pará state, Brazil, between December 2005 and June 2007 (seven scientific expeditions), before the action of mineral extraction be launched. Insects belonging to the families Ceratopogonidae, Culicidae, Psychodidae, and Simulidae were collected using the miniature CDC and Shannon light traps, and human bait; sylvan vertebrates (birds, small mammals and reptilians) were also captured in the are using Shermann and Tommahwak traps. Biological specimens from insects and vertebrates were used to attempt of virus isolation in suckling mice, while the vertebrate serum samples to determine the antibody prevalence to 19 arboviruses by hemagglutination inhibition (HI) tests. A total of 44,795 (1,220 lots) hematophagous insects were collected, mostly of Psychodidae. The most abundant species of Culicidae were Haemagogus leucocelaenus and Haemgogus janthinomys; a total of 1,288 vertebrates were also captured; the rodents Proechimys guyannensis and Oryzomys capito, and the birds Turdus albicolis and Phlegopsis nigromaculata were the most prevalent species in the area. Three Tucunduba virus strains were isolated from lots of Anopheles (Nys.) sp., Culex coronator and Wyeomyia sp. HI antibodies were found to Bussuquara virus, Cacipacore virus, Icoaraci virus, Ilheus virus, Mucambo virus Rocio virus, Saint Louis encephalitis virus (SLEV), and western equine encephalitis virus. The most prevalent arbovirus by HI was the SLEV.
Acesso aberto (Open Access)
Avaliação sorológica dos antígenos micobacterianos ND-O-BSA, LID-1 E NDO-LID em pacientes com hanseníase, contatos intradomiciliares e estudantes de um município hiperendêmico da Amazônia brasileira
(Universidade Federal do Pará, 2014-04-05) MORAES, Tânia Mara Pires; SALGADO, Claudio Guedes; http://lattes.cnpq.br/2310734509396125
Despite efforts for its elimination as a public health problem, leprosy remains highly prevalence in some countries, including Brazil, specially in the state of Pará, which accounts for approximately 10% of the 400,000 new cases in Brazil during the last 10 years. To date, there is no diagnostic test to detect leprosy in its early stages, thus contributing to the maintenance of high rates of disease incidence. In this sense, the Discovery of new specific antigens of M. leprae to enable the development of new diagnostic methods may facilitate early detection of disease prior to the onset of disfigurement and nerve damage and contribute to achieving the goals of leprosy control. In this study, dermatological clinical evaluation of the participants was performed to detect new cases and blood samples were collected for antibody screening at two different timpoints, T1 (2011) and T2 (2013), two years apart. IgM anti-ND-O-BSA and IgG anti-LID-1 titers were detected by ELISA, and anti-IgM and anti-IgG were combined for detection of both in plasma samples by ELISA or also the whole blood by OrangeLife® (OL) rapid test in 79 leprosy patients, 131 household contacts and 331 students from the municipality of Breves, Pará State. Samples collected at T1 showed a high number of new cases detected, with 18.6% of household contacts and 6.1% of students diagnosed, while two years later at T2, there were 19.8% of household contacts and 9.4% of students diagnosed. At T2, 44.3% of contacts were positive for anti-ND-O-BSA, 7.8% for anti-LID-1 and 37.4% for anti-NDO-LID. Mong the students 49.5% were positive for anti-ND-O-BSA, 5.1% for anti-LID-1 and 37% for anti-NDO-LID. The association between antigens showed a strong correlation to ND-O-BSA and NDO-LID. Positivity of the OL rapide test was 44.3% for newly diagnosed MB cases (BT majority), in students was 47.4% and 36.3% in household contacts, with poor agreement with ELISA anti-NDO-LID. At follow-up (T2), the percentage of new cases was 35% and the largest number was identified among individuals positive for anti-ND-O-BSA. The data show a high incidence in contacts and students through active search and serologic follow-up, and we concluded that the antigen ND-O-BSA was more sensitive in the ELISA assay for identifying new cases in populations endemic.
Acesso aberto (Open Access)
Comparação das técnicas de ELISA indireto e Imunofluorescência indireta na detecção de anticorpos anti-Neospora caninum em búfalas (Bubalus bubalis)
(2013-04) SILVA, Sandro Patroca da; MOTA, Rinaldo Aparecido; FARIA, Eduardo Bento de; CASSEB, Alexandre do Rosário; CASSEB, Livia Medeiros Neves; DIAS, Hilma Lúcia Tavares
To compare two serologic tests for detection of antibodies against Neospora caninum in sera from buffaloes, samples were collected from 288 buffaloes of 2 to 10 years of age. To identify the presence of IgG, anti-N. caninum was used for the indirect immunofluorescence assay (IFAT), with title 200 as the cutoff, and for the immunoenzymatic test (indirect ELISA), considering as positive samples with ratio S/P >0.5. There were 153 (53.12%) animals positive for N. caninum by IFAT, whilst 50 (17.36%) animals were reactive in ELISA. The presence of antibodies anti-N. caninum demonstrates that the parasite is circulating among buffaloes raised in Pará State, Brazil. ELISA and IFAT tests could be used to diagnose immunoglobulins against this agent. However there was a weak correlation (Kappa = 0.36) between both tests, considering the IFAT as the gold standard.
Acesso aberto (Open Access)
Estudo soroepidemiológico da infecção pelo vírus da hepatite B entre portadores do vírus da imunodeficiência humana/SIDA na cidade de Belém, Pará - Brasil
(Universidade Federal do Pará, 2004) MONTEIRO, Maria Rita de Cássia Costa; NASCIMENTO, Margarida Maria Passeri do; PASSOS, Afonso Dinis Costa; FIGUEIREDO, José Fernando de Castro
The objective of this investigation was to study the prevalence of hepatitis B virus infection in a sample of 406 adult patients with human immunodeficiency virus infection who attended at the public health care in the city of Belém, Pará, Brazil, as well as analyzing possible risk factors for hepatitis B virus infection. The overall prevalence of hepatitis B virus infection was 51% (CI: 46.1 - 55.8), with 7.9% (CI: 5.3 - 10.5) for HBsAg, 45.1% (CI: 40.3 - 49.9) for anti-HBc and 32.3% (CI: 27.5 - 36.8) for anti-HBs. After adjustment using logistic regression, hepatitis B serological markers were associated with the following variables: age, marital status and sexual preference. The frequency of hepatitis B markers was 28.7% in heterosexuals and 68.8% in homo/bisexuals (CI: 3.50 - 9.08; OR: 5.63; p=0.000). In married people the frequency was 31% and 58.7% in single people (CI: 1.29 - 3.63); OR: 2.16; p=0.003). Multivariate analysis showed no association between hepatitis B virus infection and illicit injectable drug use.
Acesso aberto (Open Access)
Evaluation of agreement between tests for the diagnosis of leprosy
(Sociedade Brasileira de Patologia Clínica, 2017-04) SILVA, Alison Rodrigues da; QUEIROZ, Marcos Fabiano de Almeida; ISHIKAWA, Edna Aoba Yassui; SILVESTRE, Maria do Perpétuo Socorro Amador; XAVIER, Marilia Brasil
Introduction: Leprosy is a chronic infectious disease caused by the intracellular parasite Mycobacterium leprae. The diagnosis is essentially clinical, based on symptoms, skin exam, peripheral nerves and epidemiological history. Laboratory tests are carried out to complement the result of clinical diagnosis, or even serving as a confirmatory method. Objective: To investigate the positivity and agreement between skin smear, enzyme-linked immunosorbent assay (ELISA) with synthetic antigen ND-O-BSA, ML Flow test and polymerase chain reaction (PCR) for detection of Mycobacterium leprae in new cases of leprosy. Methods: We conducted a case series study assessing a convenience sample of 39 new cases of leprosy and a control group of 18 household contacts in Belém (PA) and in Igarapé-Açu (PA) from March 2014 to September 2015. Results: The agreement between ELISA, ML Flow and PCR tests combinations showed slight to absent reproducibility (Kappa ≤ 0.24). The results showed greater sensitivity in PCR assay, with higher positivity in multibacillary cases. The ELISA test showed low positivity, even in multibacillary cases, resulting in no reaction to paucibacillary cases and household contacts. Conclusion: The high sensitivity of PCR decreases the agreement with other tests.
Acesso aberto (Open Access)
Freqüência de anticorpos homólogos anti-Borrelia burgdorferi em eqüinos na mesorregião metropolitana de Belém, Estado do Pará
(2009-03) GALO, Katiany Rocha; FONSECA, Adivaldo Henrique da; MADUREIRA, Renata Cunha; BARBOSA NETO, José Diomedes
Spirochaetes transmitted by ticks are microorganisms of worldwide distribution, which infect wild, domestic animals and human beings. A total of 300 equine sera from four municipalities: Ananideua (58), Belém (61), Castanhal (131), and Santa Izabel do Pará (50), were evaluated for Borrelia burgdorferi by an Elisa test. There were no significant differences (P<0.05) among municipalities, breed, sex or husbandry. A total of 80 (26.7%) horses were B. burgdorferi positive with titles of 1:800, 72 (90%) horses, 1:1.600, 6 (7.5%) horses, and 1:3.200, 2 (2.5%) horses. The results were similar to those in the USA, where related frequencies ranged from 7 to 75% in asymptomatic seropositive horses. The presence of anti-B.burgdorferi homologous antibodies in horses from four municipalities in the metropolitan mesorregion of Belém suggests the possibility of occurrence of human cases in the region.
Acesso aberto (Open Access)
Infecções pelos vírus das hepatites B e C e o carcinoma hepatocelular na Amazônia oriental
(2004) MIRANDA, Esther Castello Branco Mello; MÓIA, Lizomar de Jesus Maués Pereira; AMARAL, Ivanete do Socorro Abraçado; BARBOSA, Maria Silvia de Brito; CONDE, Simone Regina Souza da Silva; ARAÚJO, Marialva Tereza Ferreira de; CRUZ, Ermelinda do Rosário Moutinho da; DEMACHKI, Samia; BENSABATH, Gilberta; SOARES, Manoel do Carmo Pereira
In order to contribute to a better understanding of the possible role of hepatits B and C in the etiopathogenis of HCC in the East Amazon, there were studied 36 patients in Belém/PA. Serological hepatitis markers were evaluated and polymerase chain reaction assays were used to detect HBV-DNA and HCV-RNA. Alcohol abuse was observed in 33.3% and cirrhosis in 83.3%. In 88.9% of the sample, one or more hepatitis B markers were positive. Also, 8.3% those patients had anti-HCV simultaneously positive. The HBsAg serological test was positive in 58.3%; anti-HBc in 86%; anti-HBe in 85.7%; anti-HBe in 9.5%; IgM anti-HBc in 57.1%. The HBV DNA was found in 37.7% and in 65% of the HBsAg positive. The HCV RNA was detected in 8.5% and in 100% of' the patients positive to anti-HCV. The AFP was above the normal value in 88.9% of patients, with levels up to 400ng/ml in 75% of them. In conclusion, hepatitis B virus infection seems to be important in the etiology of HCC and improving measures such immunization and screening in the risk population should be emphasyzed.
Acesso aberto (Open Access)
Inquérito sorológico da infecção pelo vírus linfotrópico humano de células T (HTLV) em comunidades amazônicas localizadasnas margens do Lago da Usina Hidrelétrica de Tucurui/PA
(Universidade Federal do Pará, 2010-12-20) FALCÃO, Luiz Fábio Magno; QUARESMA, Juarez Antônio Simões; http://lattes.cnpq.br/3350166863853054
The aim of this study was to evaluate seroprevalence lymphotropic virus human T cells (HTLV) in individuals living in communities of Alcobaça e Ararão, located on the shores of lake Hydroelectric Plant Tucuruí / PA. Serum samples from 657 individuals were analyzed by immunoenzymatic assays the type of ELISA (Symbioys ®). There was a significant prevalence of HTLVI / II (4.7%) in the study population and the demographic characteristics reflects most of them are older than 40 years (p = 0.0239), unmarried (p = 0.035), resident for over 10 years in the catchment area of the study (p = 0.0027) and educational level was equal to or less than 4 years (p = 0.0308). There was no statistically significant difference when analyzing variables such as sex, birth, blood transfusion and previous surgery. The prevalence for HTLV is considered high according to current literature, justifying the screening for this virus at the study sites in order that we can better understand the epidemiology of HTLV transmission in the population.
Acesso aberto (Open Access)
Seroprevalence for brucellosis and leptospirosis in dogs from Belém and Castanhal, State of Pará, Brazil
(Instituto Nacional de Pesquisas da Amazônia, 2015-09) PAZ, Giselle Souza da; ROCHA, Katarine de Souza; LIMA, Michele de Souza; JORGE, Ediene Moura; PANTOJA, José Carlos Figueiredo; MORAES, Carla Cristina Guimarães de; LANGONI, Helio
Brucellosis and leptospirosis are widely spread bacterial infections and dogs are the most important source of infection and reservoir for diseases. Dogs can disseminate the agents in the environment and transmit them to humans and/or other animals. The objective of this study was assess the occurrence of reactive to antibodies anti-Leptospira spp., Brucella canis and B. abortus in Belém and Castanhal, State of Pará, Amazon, Brazil. A total of 156 samples were randomly collected in the city of Belém and 158 samples in Castanhal. The anti-B. canis antibodies research was performed by Agar Gel Immunodiffusion (AGID) with and without 2-mercaptoethanol serum treatment (AGID-2ME). To assess the anti-B. abortus antibodies, the technique of Fast Seroagglutination with buffered acidified plate antigen (BAPAT) was used. For anti-Leptospira spp. antibodies research, the Microscopic Agglutination Technique (MAT) was used. No animal reacted to Brucella abortus and one animal was reactive to B. canis at the AGID, but it was negative to the AGID-2ME test. Seventeen percent of dogs (47/274) presented anti-Leptospira spp. antibodies, with prevalence of serovar Canicola. The dogs from Belém and Castanhal are not source of infection for B. abortus and B. canis, however, they are reservoirs for different serovars of Leptospira spp.
Acesso aberto (Open Access)
Virological and serological diagnosis of rabies in bats from an urban area in the brazilian Amazon
(Universidade Federal do Pará, 2015-12) OLIVEIRA, Rubens Souza de; COSTA, Lanna Jamile Corrêa da; ANDRADE, Fernanda Atanaena Gonçalves de; UIEDA, Wilson; MARTORELLI, Luzia Fatima Alves; KATAOKA, Ana Paula de Arruda Geraldes; ROSA, Elizabeth Salbé Travassos da; VASCONCELOS, Pedro Fernando da Costa; PEREIRA, Armando de Souza; FERNANDES, Marcus Emanuel Barroncas; CARMO, Antônio Ismael Barros do
The outbreaks of rabies in humans transmitted by Desmodus rotundus in 2004 and 2005, in the northeast of the Brazilian State of Para, eastern Amazon basin, made this a priority area for studies on this zoonosis. Given this, the present study provides data on this phenomenon in an urban context, in order to assess the possible circulation of the classic rabies virus (RABV) among bat species in Capanema, a town in the Amazon basin. Bats were collected, in 2011, with mist nets during the wet and dry seasons. Samples of brain tissue and blood were collected for virological and serological survey, respectively. None of the 153 brain tissue samples analyzed tested positive for RABV infection, but 50.34% (95% CI: 45.67-55.01%) of the serum samples analyzed were seropositive. Artibeus planirostris was the most common species, with a high percentage of seropositive individuals (52.46%, 95% CI: 52.31 52.60%). Statistically, equal proportions of seropositive results were obtained in the rainy and dry seasons (c2 = 0.057, d.f. = 1, p = 0.88). Significantly higher proportions of males (55.96%, 95% CI: 48.96-62.96%) and adults (52.37%, 95% CI: 47.35-57.39%) were seropositive. While none of the brain tissue samples tested positive for infection, the high proportion of seropositive specimens indicates that RABV may be widespread in this urban area.