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Navegando por Assunto "Stem cells"

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    Biotecnologias, subjetivação e psicologias: mercado de células-tronco do cordão umbilical
    (Associação Brasileira de Psicologia Social, 2017) GALINDO, Dolores Cristina Gomes; RODRIGUES, Renata Vilela; LEMOS, Flávia Cristina Silveira; ALMEIDA, Leila Cristina da Conceição Santos
    This article aims to analyze production practices of bio-social subjectivity through the stem cell market made through risk management and biopolitics future precautions, within the family and the parent-child relationship. The interest of psychology from biotechnological innovation can be viewed both in critical questioning conducted by scientific field on ways of subjectivities that emerge from the coupling between biotech and body, and in your professional field, through genetic counseling psychotherapy. In biopolitical strategies of control and manipulation of umbilical cord stem cells show up subjectivities that emerge in a complex network of tecnobiológica production.
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    Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2018-05) BRITO, Felipe Nunes; VENDRAMIN, Fabiel Spani; LOPES, Cinthia Távora de Albuquerque; COSTA, Mayra Pauline Ribeiro; OHASHI, Otavio Mitio; MAIA, José Guilherme Soares; FERREIRA, Lydia Masako; SILVA, Joyce Kelly do Rosário da; MIRANDA, Moysés dos Santos
    Purpose: To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells. Methods: Stem cells were isolated from human adipose tissue by liposuction. After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/mL, 5.0 μg/mL, and 25.0 μg/mL). Cells were analyzed by the MTT assay at passage 1 (P1), and cell proliferation of control and 1 μg/mL groups was determined with a hemocytometer at P2 and P3. Results: Viability of the essential oil-treated cells was significantly higher than the control group at P1 (p = 0.0008). The treatment with the oil, at a concentration of 1 µg/mL, led to increases of 24.8% at P1 and 43.0% at P3 in the rate of cell proliferation compared with control cells. Conclusion: Supplementing culture medium with essential oil of Lippia origanoides increased cell proliferation, especially at later passages.
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    Stem cells from adipose tissue improve the time of wound healing in rats
    (Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2016-12) OHASHI, Camila Melo; CALDEIRA, Fabio Alves Morikawa; FEITOSA JUNIOR, Denilson José Silva; VALENTE, André Lopes; DUTRA, Paulo Roberto Witter; MIRANDA, Moysés dos Santos; SANTOS, Simone do Socorro Damasceno; BRITO, Marcus Vinicius Henriques; OHASHI, Otávio Mitio; YASOJIMA, Edson Yuzur
    PURPOSE: To evaluate the Adipose Stem Cells (ACS) therapy efficacy on the time and quality of wound healing process in rats. METHODS: Nine male Wistar rats were randomly distributed into three groups I) 7 days of healing; II) 14 days of healing; III) 21 days of healing. Four incisions were made on the dorsal surface of each rat and then treated with intralesional ACS, meloxicam, and no treatment and ACS+meloxicam. Macroscopic evaluation was measured by percentage of healing and histopathological by hematoxylineosin was performed. RESULTS: All groups have the wound reduced during the three weeks (p<0.001) and after 14 days of healing had greater reduction than others. Wounds treated with ASC had accelerated healing in relation to no treatment and only meloxicam (p<0.001), excepting the ASC+Meloxicam that was similar (p=0.13). There was no difference in histopathological analysis between lesions. CONCLUSION: Adipose stem cell have benefits in reducing time of healing of experimental model of wound in rats, observed 7 days of after application.
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