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Navegando por Assunto "Tissue engineering"

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    Desenvolvimento de Redes Semi-Interpenetrantes de PCL-pHEMA-copaíba para potencial uso com scaffolds na Engenharia de Tecidos
    (Universidade Federal do Pará, 2024-02-22) LIMA, Tainara de Paula de Lima; PASSOS, Marcele Fonseca; http://lattes.cnpq.br/0588450144351187; https://orcid.org/0000-0002-5616-2127
    Tissue engineering is an alternative to replace organs and tissues in the biological system affected by an illness. Therefore, it is necessary to study the material used as a scaffold in depth. Among the materials in this area, polymers and hydrogels stand out, such as poly (ε-caprolactone) (PCL) and poly (2-hydroxyethyl methacrylate) (PHEMA), respectively. PCL is a bioresorbable, biodegradable, and biocompatible polymer. However, it is hydrophobic.On the other hand, pHEMA is a biocompatible and hydrophilic hydrogel but does not show good degradability. Furthermore, it is possible to intersperse bioactive compounds through the use of Amazonian vegetable oils in these structures to further enhance tissue regeneration and combat possible infections by microorganisms. Therefore, this work aimed to obtain and characterize PCL-PHEMA-copaíba semi-IPN networks for scaffolds in tissue engineering using the rotospinning technique. The results successfully demonstrated the processing of PCL fibers (with and without copaiba oil) and the formation of PCL-C-PHEMA semi-IPN networks. Gas chromatography confirmed the presence of bioactive components in copaiba essential oil, the majority being (β)-caryophyllene (40.75%). The FTIR spectrum showed interactions of the materials' functional groups, confirming the incorporation of the oil into the PCL structure and the formation of semi-interpenetrating networks. Micrographs and topographies revealed tangled and disorganized microfibers in all samples, with different diameters, porosities, and roughness. The PCL, PCL-C, and PCL-C-PHEMA samples presented fiber diameters ranging from 18.40 to 19.50 μm, 3.11 to 24.44 μm, and 6.29 to 8.14 μm, respectively. Contact angle analyses (PCL: 86.96°, PCL-C: 93.99°, PCL-PHEMA: 29.42°, and PCL-C-PHEMA: 56.02°) and swelling test (PCL: 4.49%, PCL-C: 2.73%, PCL-PHEMA: 21.57%, and PCL-C-PHEMA: 10.11%) demonstrated that the addition of the hydrogel to the PCL structure optimized the hydrophilic properties of material. The sol-gel tests indicated that the PCL-PHEMA and PCL-C-PHEMA materials presented 73.5 74.3% gel fractions. Thermograms confirmed that the material did not significantly change in thermal stability with the addition of the hydrogel and oil. Microbiological tests confirmed the antimicrobial action of copaiba oil, PCL-C-PHEMA, and PCL-C scaffolds against the gram-positive bacterium Staphylococcus aureus, with an inhibition halo of 9, 7, and 5 mm, respectively. Moreover, the cytotoxicity tests concluded that the PCL, PCL-PHEMA, and PCL-C scaffolds showed good cell viability. However, optimizing the photopolymerization process of the semi-IPN network is necessary, given that the PCL-C-PHEMA materials were moderately toxic. Finally, a new biomaterial is expected to be developed for use in tissue engineering, valuing the use of natural Amazonian resources.
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    Obtenção de filmes de colágeno de peixe para biomaterais: peixes da amazônia (Heros Severus e Cichla Temensis) e tilápia do nilo (Oreochromis Niloticus)
    (Universidade Federal do Pará, 2023-10-27) COELHO, Katherine Elice Paes Leão; LAMEIRA, Aladim Gomes; http://lattes.cnpq.br/7739057853603135; DIAS, Carmen Gilda Barroso Tavares; http://lattes.cnpq.br/2113791118142177
    The Collagen films derived from fish skin have been gaining prominence in tissue engineering, with emphasis on the large number of research on the properties of those obtained from Nile Tilapia - TN (Oreochromis niloticus).Parallel to this; the Amazon Basin has numerous species of fish, which have not yet been deeply addressed in the field of bioprospecting. In this sense, this paper sought to prepare and analyze films derived from the skins of Amazonian fish, such as Peacock Bass - TA (Cichla Temensis) and Banded Cichlid - AS (Heros Severus), comparing them with that of the African fish TN. From the results obtained, it was verified that TN was the one with the highest collagen yield. The proteins were extracted and purified to prepare aqueous solutions with polyvinyl alcohol. The films obtained by solvent evaporation showed the following traction resistance results: 327 MPa for TN; 299 MPa for AS, and 228 MPa for TA. Through scanning electron microscopy, it was possible to note that the greatest porosity was present in TN films, which received a coating of spheres and nanofibers deposited at a distance of 3 cm under tension of 6.3 KW generated in a COCKCROFT – WALTON source. Therefore, it was found that, although the TA and AS polymers showed good results, the films made from Tilapia were the ones that best corroborated those made from biomaterials for dental use. Finally, it is believed that those using Amazonian fish made it possible to reproduce methods already vehemently applied in the literature and could eventually supply the needs of market.
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