Navegando por Assunto "Tuberculose em animais"

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Acesso aberto (Open Access)
Cadeia produtiva da pecuária de corte do município de Tailândia, estado do Pará: estrutura e caracterização sanitária dos abates
(Universidade Federal do Pará, 2015) NORONHA, Gerlane Nunes; CASSEB, Alexandre do Rosário; http://lattes.cnpq.br/6525374874057337; PEREIRA, Washington Luiz Assunção; http://lattes.cnpq.br/9266281009742277; LOURENÇO JÚNIOR, José de Brito; http://lattes.cnpq.br/2919433679918544
This work aims to characterize the productive chain of beef cattle in the town of Tailândia, Pará, Mesoregion Northeast Pará, in order to identify the state of the art and emphasize the slaughter stage and prevalence of economic losses carcasses of convictions and cattle organs and confirmation by microbiological analysis of lesions suggestive of bovine tuberculosis. For evaluations, secondary data on government and institutional sites, primary data through interviews with producers, cold slaughterhouse owner, community leaders and experts from public entities were used. In second phase, primary data through spreadsheets slaughtering records and convictions of carcasses and viscera were evaluated, from March 2010 to October 2014 and found that bovine tuberculosis was one of the highest conviction pathologies (2.98%) and generating greater economic loss (71.48%). Given these results, we sought confirmation by histopathological analysis and polymerase chain reaction - PCR in order to characterize the lesions and identify the presence of Mycobacterium bovis. The histopathology showed that only 1.96% of the samples corresponded to the inflammatory process characteristic of tuberculosis and 4.25% with amplification profile for the species of M. bovis. The results of this study allow us to conclude that there is need to use complementary methods to improve diagnostic of bovine tuberculosis in slaughterhouses to increase the epidemiological disease control, streamline the inspection service and reduce economic losses generated, which will make the strongest production chain.
Acesso aberto (Open Access)
Caracterização histopatológica, imunohistoquímica e determinação molecular de Mycobacterium bovis de lesões sugestivas de tuberculose em bubalinos abatidos para consumo no estado do Amapá
(Universidade Federal do Pará, 2016-02-26) PEDROSO, Silvia Cristina da Silva; LIMA, Karla Valéria Batista; http://lattes.cnpq.br/9795461154139260; PEREIRA, Washington Luiz Assunção; http://lattes.cnpq.br/9266281009742277
The creation of buffalo is an important source of income in Amapá, an important sector in the local economy. Tuberculosis is an infectious disease of worldwide distribution, which occurs in cattle and buffaloes caused mainly by Mycobacterium bovis. The disease has debilitating chronic nature with economic impact on the production of meat and milk in different regions of the world, besides being an important zoonosis. Aiming to characterize M. bovis in suggestive of tuberculosis lesions detected in buffalo inspection in the State of Amapá, through histopathological and immunohistochemical methods (IHC) and molecular analysis, there was the collection of 109 samples suggestive of tuberculosis lesions. For histopathological analysis, we used standard techniques for paraffin by hematoxylin and eosin and Fite-Faraco. For IHC was used the method of peroxidase-antiperoxidase (PAP) with a polyclonal rabbit antiserum against M. bovis (Dako B0124). For molecular analysis, mycobacterial DNA extraction was directly performed lymph node and treated by the phenolchloroform method through two amplification protocols (conventional PCR and Nested- PCR). Histologically, 94.5% (103/109) had typical granulomatous tissue reaction tuberculoide injury (LT), while in 5.5% (06/109) without features of tuberculoid granulomas (p <0.0001). The results obtained by IHC among the positive cases by histopathology, all had some kind of injury classified in several stages by histopathology, and observed that most of them 82.2% (74/90; p <0.0001) was only stage IV. Among the negative cases, necrotic lesions presenting only the last stage of development (IV) were also frequently 52.5% (10/19) and 31.5% (06/19) were also negative in the histopathologic ( p = 0.0030). Of the 103 positive samples for histopathology, 73.8% (76/103) were PCR positive reaction to the genus Mycobacterium and 64.1% (66/103) had amplification for the RD4 region, confirming the deletion, exclusive of the species M. bovis. The results showed that tuberculosis is present in buffalo herds of Amapá and regions of Marajó in Pará, representing a serious risk to public health.
Acesso aberto (Open Access)
Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR
(2014-06) ARAÚJO, Cristina Pires de; OSÓRIO, Ana Luiza Alves Rosa; JORGE, Klaudia dos Santos Gonçalves; RAMOS, Carlos Alberto do Nascimento; SOUZA FILHO, Antonio Francisco de; VIDAL, Carlos Eugênio Soto; VARGAS, Agueda Palmira Castagna de; ROXO, Eliana; ROCHA, Adalgiza da Silva; SUFFYS, Philip Noel; FONSECA JÚNIOR, Antônio Augusto; SILVA, Marcio Roberto; BARBOSA NETO, José Diomedes; CERQUEIRA, Valíria Duarte; ARAÚJO, Flábio Ribeiro de
Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. Regarding the analytical sensitivity, DNA of the M. bovis AN5 strain was detected up to 1.5 pg by nested-PCR, whereas DNA of M. tuberculosis H37Rv strain was detected up to 6.1 pg. The nested-PCR system showed 100% analytical specificity for MTC when tested with DNA of reference strains of non-tuberculous mycobacteria and closely-related Actinomycetales. A clinical sensitivity level of 76.7% was detected with tissues samples positive for MTC by means of the culture and conventional PCR. A clinical specificity of 100% was detected with DNA from tissue samples of cattle with negative results in the comparative intradermal tuberculin test. These cattle exhibited no visible lesions and were negative in the culture for MTC. The use of the nested-PCR assay to detect M. tuberculosis complex in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis.