Navegando por Assunto "Urease"

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Acesso aberto (Open Access)
Atividade antifúngica da nimesulida isolada ou em associação com a terbinafina contra fungos dermatófitos e seu provável mecanismo de ação in vitro
(Universidade Federal do Pará, 2015) MATOS, Rafaelle Fonseca de; MONTEIRO, Marta Chagas; http://lattes.cnpq.br/6710783324317390
The dermatophytes has shown resistance to current antifungal agents such as Terbinafine, and this has led to research into new compounds as an alternative therapy. Thus, this study evaluated the action of non-steroidal antiinflamatory (NSAIDs) Nimesulide isolated and combined with Terbinafine against dermatophytes, dependence on Prostaglandins this mechanism of action and the effect of Nimesulide in the production of urease and fungal viability. The tests based were on CLSI, clinical and laboratory standards institute - reference method for microdilution test ground for antifungal susceptibility against filamentous fungi (M38-A standard). For clinical isolates of the species Trichophyton mentagrophytes, Trichophyton rubrum, Epidermophyton floccosum and Microsporum canis, the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) varied in the range> 400 ug / ml -> 0.112 g / ml. However, the results showed that the concentration of 0.002 mg / ml of Nimesulide was able to inhibit the growth of T. mentagrophytes ATCC 9533 and the concentration of 0.008 mg / ml was fungicidal for the same strain. Same values were find for Terbinafine on this strain. The inhibition of fungal growth by Nimesulide was reverse with application of exogenous prostaglandin E2 (PGE2). The urease test showed that T. mentagrophytes ATCC 9533 produces the enzyme, but no inhibition of fungal viability. In the nimesulide / Terbinafine association inhibition occurred in a ratio of 9: 1, or 0.0002 g / ml of Terbinafine and 0.0018 ug / ml of Nimesulide inhibited fungal growth but this result was the indifferent Fractional inhibition index. Nimesulide showed antifungal activity against dermatophytes in low concentrations, but other studies must be performed with the aid of molecular modeling to improve the targeting compound.
Acesso aberto (Open Access)
Gender, age, endoscopic findings, urease and Helicobacter pylori: all uncorrelated within a sample of a high gastric cancer prevalence population in Amazon
(Instituto Brasileiro de Estudos e Pesquisas de Gastroenterologia e Outras Especialidades, 2019-09) MIRANDA, Ariney Costa de; CALDATO, Cássio; SAID, Mira Nabil; LEVY, Caio de Souza; TEIXEIRA, Cláudio Eduardo Corrêa; QUARESMA, Juarez Antônio Simões
BACKGROUND: It is widely assumed that gender, age, gastritis and Helicobacter pylori , all have some degree of cor-relation and, therefore, can synergistically lead to the development of gastric cancer. OBJECTIVE: In this cross-sectional study, we expected to observe the above mentioned correlation in the analysis of medical records of 67 patients of both sexes (female, n=44), mean age ± standard deviation: 41±12 years old, all from Belém (capital of Pará State, Brazilian Amazon), a city historically known as one with the highest gastric cancer prevalence in this country. METHODS: All patients were submitted to upper gastrointestinal endoscopy for gastric biopsy histopathological analysis and rapid urease test. All diagnoses of gastritis were recorded considering its topography, category and the degree of inflammatory activity, being associated or not associated with H. pylo-ri infection. RESULTS: The results show that no statistically relevant associations were found among the prevalences of the observed variables. CONCLUSION: The authors hypothesize that observed risk factors associated to gastric cancer might be lesser syner-gistic than is usually expected.