Navegando por Assunto "Wound healing"

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Acesso aberto (Open Access)
Efeito de uma espécie do gênero Varronia sobre a viabilidade celular, atividade antimicrobiana, toxicidade dérmica aguda e o processo de cicatrização (in vitro e in vivo)
(Universidade Federal do Pará, 2019-09-09) RIBERA, Paula Cardoso; FONTES JÚNIOR, Enéas de Andrade; http://lattes.cnpq.br/7056265073849866; https://orcid.org/ 0000-0002-6186-9581
Tissue damage, particularly to the skin, results in damage to cell structures, layers, and lineages to the fullest extent. Under these conditions, wound healing is the physiological process responsible for tissue repair. Inflammation is an important stage in tissue repair and; therefore, a strong target for clinical studies. The species Varronia multispicata is popularly used for the treatment of bruises, with recently discovered anti-inflammatory and analgesic effects. The present work aimed to investigate the effect of aqueous extract of Varronia multispicata leaves (VAR01) on cell viability, antimicrobial analysis, dermal toxicity, and in vitro and in vivo healing. In the in vitro assays, there were evaluated the cell viability in BALB/c 3T3 murine fibroblasts, the antimicrobial action by the microdilution method for determination of minimum inhibitory concentration and petri dish culture technique for the minimum bactericidal concentration. Healing assays were performed in cultured fibroblast monolayers. For in vivo assays in the dermal toxicity test, female Wistar rats were used and divided into the following groups: saline, 100mg/ml, 200mg/ml, and 1000mg/ml of VAR01; as for healing evaluation, Mus musculus mice were used and divided into 4 groups: sham, negative control, treated (VAR01 10%), and positive control (Dersani®). V. multispicata kept the cells viable for 24h, with reduction of fibroblasts in the 48h period at a concentration of 500 µg/ml. It showed no antimicrobial activity, presented in vitro and in vivo injury contraction capacity, did not promote death or behavioral changes, did not cause changes in water and feed intake, weight gain, relative weight, and organ histological analysis, showed a reduction in alkaline phosphatase concentrations in the group treated with 100 mg/ml extract when compared as control group. It was also revealed a reduction in alanine aminotransferase levels in the 100 mg/ml extract-treated group when compared to the control group. However, a significant increase in TGP concentrations was found in the 200 mg/ml group when compared to the control group. While assessing the degree of irritation, VAR01 did not show an irritant profile when administered acutely topically. Therefore, the extract is safe and of low toxicity, promising in the process of tissue regeneration with possible modulation in the inflammatory pathway, being a stimulating result for the following steps of biological activity evaluation and elucidation of the healing process.
Acesso aberto (Open Access)
Stem cells from adipose tissue improve the time of wound healing in rats
(Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, 2016-12) OHASHI, Camila Melo; CALDEIRA, Fabio Alves Morikawa; FEITOSA JUNIOR, Denilson José Silva; VALENTE, André Lopes; DUTRA, Paulo Roberto Witter; MIRANDA, Moysés dos Santos; SANTOS, Simone do Socorro Damasceno; BRITO, Marcus Vinicius Henriques; OHASHI, Otávio Mitio; YASOJIMA, Edson Yuzur
PURPOSE: To evaluate the Adipose Stem Cells (ACS) therapy efficacy on the time and quality of wound healing process in rats. METHODS: Nine male Wistar rats were randomly distributed into three groups I) 7 days of healing; II) 14 days of healing; III) 21 days of healing. Four incisions were made on the dorsal surface of each rat and then treated with intralesional ACS, meloxicam, and no treatment and ACS+meloxicam. Macroscopic evaluation was measured by percentage of healing and histopathological by hematoxylineosin was performed. RESULTS: All groups have the wound reduced during the three weeks (p<0.001) and after 14 days of healing had greater reduction than others. Wounds treated with ASC had accelerated healing in relation to no treatment and only meloxicam (p<0.001), excepting the ASC+Meloxicam that was similar (p=0.13). There was no difference in histopathological analysis between lesions. CONCLUSION: Adipose stem cell have benefits in reducing time of healing of experimental model of wound in rats, observed 7 days of after application.