Parâmetros da escototaxia como modelo comportamental de ansiedade no paulistinha (danio rerio, cyprinidae, pisces)

Abstract

Certain teleost fishes present a behavioral trait of scototaxis, the preference for dark environ- ments and not bright ones. The present work tried to evaluate some parameters of the explora- tory behavior of the zebrafish (Danio rerio Hamilton 1822) in the black/white tank, aiming to establish the reliability of measures in different contexts. White compartment avoidance presents a biphasic pattern, with an increase in avoidance preceding a decrease (Experiment 1). This same avoidance does not habituate to repeated exposure, independently of the inter- session interval, on the contrary of total locomotion (Experiments 2 and 3); forced exposure to the white compartment does not alter the subsequent exploratory behavior (Experiment 4). These results suggest that novelty is not the controlling dimension of scototaxis; besides, these results also suggest that the preference for the Black compartment is not caused simply by avoidance of the white compartment, although this certainly has an important role. The role of the aversiveness of the white compartment was assessed in a second series of experi- ments. The illumination above the white compartment is an anxiogenic factor, since increas- ing it decreases the time spent in the white compartment without affecting total locomotion (Experiment 5). This phenomenon seems to be due to a decrease in the capacity to camouf- lage with the substrate (crypsis), since altering the color of the white compartment to grey increases its exploration, while altering the color of the black compartment to grey increases the time spent in the white compartment (Experiment 6). Besides, the increase in the propor- tion of the apparatus occupied by the white compartment (from 50% to 75%) decreases the time spent in it (Experiment 7). These results suggest that the white compartment is aversive, and therefore the preference for darkness is not caused simply by positive reinforcing proper- ties of the black compartment. Taken together, the results of both series of experiments sug- gest that scototaxis results from an approach-avoidance conflict. Experiment 8 represents a common environmental manipulation which alters anxiety in rodents, environmental enrich- ment; here, animals raised in an enriched aquarium for two weeks present less white avoid- ance. It is concluded that scototaxis has good construct validity.