Epidemiologia molecular do Vírus linfotrópico de células T humanas - HTLV 1/2 no Estado do Amapá-Brasil

Abstract

The geographical distribution of the infection by human T-cell lymphotropic virus 1/2 – HTLV 1 / 2 is extensive, nevertheless, there are areas that are more endemic and have more particularities depending on the HTLV type. The HTLV 1 shows a bigger occurrence in south west of Japan, Caribbean, Central America, in different regions of South America and in parts of Central, as well as in western Africa, while HTLV-2, seems to assault distinct groups of people, such as Indians native people from North, Central and South America, Pigmies from Central Africa, Mongols in Asia as well as in infecting drug users. The present work had as objective describing HTLV molecular epidemiology, in three different populations the State of Amapá, they are: HIV/IDS positive patients, Afro-descenig population and individuals assisted of Public Health Center Laboratory of Amapá – LACEN-AP, directed for diagnosis of HTLV. The samples were tested for the presence of virus using serological (ELISA and Western blot) and molecular assays (gene amplification and restriction fragment length polymorphism from pX and env for the analysis of polimorfismo o restriction fragments for endonuclease action. The obtained results in different populations are In the population of HIV infected people, all the samples were negative; in the Afro-desceding population, only one sample was positive confirmed by serological test (ELISA), but negative according to western blot test and submitted to the molecular analysis, there wast not amplification. However, among samples of individuals directed for diagnosis of HTLV, 06 (six) were positive, 5(five) out of 6/them were confirmed by western blot test. The molecular result demonstrated the presence of HTLV-1.