Production of medium-chain-length polyhydroxyalkanoate by Pseudomonas oleovorans grown in sugary cassava extract supplemented with andiroba oil

dc.creatorSILVA, Diego Aires da
dc.creatorANTONIO, Regina Vasconcellos
dc.creatorROSSI, José Márcio
dc.creatorPENA, Rosinelson da Silva
dc.date.accessioned2015-05-18T16:39:00Z
dc.date.available2015-05-18T16:39:00Z
dc.date.issued2014-12
dc.description.abstractPseudomonas oleovorans were grown on sugary cassava extracts supplemented with andiroba oil for the synthesis of a mediumchain- length polyhydroxyalkanoate (PHAMCL). The concentration of total sugars in the extract was approximately: 40 g/L in culture 1, 15 g/L in cultures 2 and 3, and 10 g/L in culture 4. Supplementation with 1% andiroba oil and 0.2 g/L of (NH4)2HPO4 was performed 6.5 hours after growth in culture 3, and supplementation with the same amount of andiroba oil and 2.4 g/L of (NH4)2HPO4 was performed at the beginning of growth in culture 4. The synthesis resulted mainly in 3-hydroxy-decanoate and 3-hydroxy-dodecanoate units; 3-hydroxy-butyrate, 3-hydroxy-hexanoate; and 3-hydroxy-octanoate monomers were also produced but in smaller proportions. P. oleovorans significantly accumulated PHAMCL in the deceleration phase of growth with an oxygen limitation but with sufficient nitrogen concentration to maintain cell growth. The sugary cassava extract supplemented with andiroba oil proved to be a potential substrate for PHAMCL production.pt_BR
dc.identifier.citationSILVA, Diego Aires da et al. Production of medium-chain-length polyhydroxyalkanoate by Pseudomonas oleovorans grown in sugary cassava extract supplemented with andiroba oil. Food Science and Technology (Campinas), Campinas, v. 34, n. 4, p. 738-745, out./dez. 2014. Disponível em: <http://www.scielo.br/pdf/cta/v34n4/v34n4a14.pdf>. Acesso em: 15 maio 2015. <http://dx.doi.org/10.1590/1678-457X.6465>.pt_BR
dc.identifier.issn1678-457X
dc.identifier.urihttps://repositorio.ufpa.br/handle/2011/6685
dc.language.isoengpt_BR
dc.rightsAcesso Abertopt_BR
dc.subjectBiopolimerospt_BR
dc.subjectCarapa guianensispt_BR
dc.subjectManihot esculentapt_BR
dc.subjectElevação de arpt_BR
dc.subjectEngenharia de alimentos
dc.titleProduction of medium-chain-length polyhydroxyalkanoate by Pseudomonas oleovorans grown in sugary cassava extract supplemented with andiroba oilpt_BR
dc.typeArtigo de Periódicopt_BR

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