Caracterização comportamental e eletroencefalográfica das convulsões induzidas pelo cunaniol e acetato de cunaniol extraídos das folhas de Clibadium sylvestre, um modelo de convulsão generalizada experimental em ratos (Wistar)

Abstract

The Clibadium sylvestre is largely distribued in the Amazon region, where is know as cunambi or cunhambi, and its ingestion causes inebriation or even fish´s death, demonstrating ichthyotoxic property. The compounds existing in the leaf of Clibadium sylvestre are powerful of central nervous system stimulants, its leafs contain potential convulsivant substances. The electroencephalographic changes, seizure and drug effects on seizure behavior control were studied as well as metabolic pathway of compounds cunaniol acetate and cunaniol. The work was performed with adult male Wistar rats, treated with DE50 of 2,92 mg/kg or DL50 of 3,64 mg/kg of cunaniol, administration route used was intraperitoneal. After cunaniol administration, the seizure evolution was observed, it allows to classify them according to the presentation intensity relate to cunaniol plasma concentration. The eletroencefalografic parameters of the drugs action on the seizure control and the clinic characteristic were determined and evaluated. The plasma analysis obtained by liquid chromatography after the application of convulsivant substances indicates that the cunaniol acetate undergoes deacetylation giving rise to cunaniol, drug responsible for convulsive state. Data electrocorticography has been shown five different patterns of tracks during recording with 4 hours remaining changes outlined by 12 hours after application. Among the drugs used to prevent the onset of seizures, the most effective were diazepam, phenobarbital and ketamine. The convulsive behavior was classified into five stages. For the occurrence of stages 4 and 5 there was no statistical differences regarding plasma cunaniol.