Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil

dc.creatorLAURENTINO, Rogério Valois
dc.creatorLOPES, Ivina Giselle Lima
dc.creatorAZEVEDO, Vânia Nakauth
dc.creatorMACHADO, Luiz Fernando Almeida
dc.creatorMOREIRA, Márcio Ronaldo Chagas
dc.creatorSOUZA, Lia Lobato Batista de
dc.creatorISHAK, Marluísa de Oliveira Guimarães
dc.creatorISHAK, Ricardo
dc.creatorVALLINOTO, Antonio Carlos Rosário
dc.date.accessioned2013-04-06T22:39:44Z
dc.date.available2013-04-06T22:39:44Z
dc.date.issued2005-07
dc.description.abstractThe present work evaluated the epidemiology of human immunodeficiency virus 1/human T-cell lymphotropic virus (HIV-1/HTLV) coinfection in patients living in Belém (state of Pará) and Macapá (state of Amapá), two cities located in the Amazon region of Brazil. A total of 169 blood samples were collected. The sera were tested by enzyme-linked immunosorbent assay to determine the presence of antibodies anti-HTLV-1/2. Confirmation of infection and discrimination of HTLV types and subtypes was performed using a nested polymerase chain reaction targeting the pX and 5' LTR regions, followed by restriction fragment length polymorphism and sequencing analysis. The presence of anti-HTLV1/2 was detected in six patients from Belém. The amplification of the pX region followed by RFLP analysis, demonstrated the presence of HTLV-1 and HTLV-2 infections among two and four patients, respectively. Sequencing HTLV-1 5' LTR indicated that the virus is a member of the Cosmopolitan Group, Transcontinental subgroup. HTLV-2 strains isolated revealed a molecular profile of subtype HTLV-2c. These results are a reflex of the epidemiological features of HIV-1/HTLV-1/2 coinfection in the North region of Brazil, which is distinct from other Brazilian regions, as reported by previous studies.pt_BR
dc.identifier.citationLAURENTINO, R. V., et al. Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 4, p. 371-376, jul. 2005. Disponível em: <http://www.scielo.br/pdf/mioc/v100n4/v100n4a06.pdf>. Acesso em: 03 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762005000400006>.pt_BR
dc.identifier.issn1678-8060
dc.identifier.issn0074-0276
dc.identifier.urihttps://repositorio.ufpa.br/handle/2011/3643
dc.language.isoengpt_BR
dc.rightsAcesso Aberto
dc.subjectHIV-1pt_BR
dc.subjectVírus linfotrópico de células T humanas tipo 1pt_BR
dc.subjectDiagnóstico laboratorialpt_BR
dc.subjectEpidemiologia molecularpt_BR
dc.subjectVírus 2 linfotrópico T humanopt_BR
dc.subjectBelém - PApt_BR
dc.subjectMacapá - APpt_BR
dc.subjectPará - Estadopt_BR
dc.subjectAmapá - Estadopt_BR
dc.subjectAmazônia brasileirapt_BR
dc.titleMolecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazilpt_BR
dc.typeArtigo de Periódico

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