Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil
dc.creator | LAURENTINO, Rogério Valois | |
dc.creator | LOPES, Ivina Giselle Lima | |
dc.creator | AZEVEDO, Vânia Nakauth | |
dc.creator | MACHADO, Luiz Fernando Almeida | |
dc.creator | MOREIRA, Márcio Ronaldo Chagas | |
dc.creator | SOUZA, Lia Lobato Batista de | |
dc.creator | ISHAK, Marluísa de Oliveira Guimarães | |
dc.creator | ISHAK, Ricardo | |
dc.creator | VALLINOTO, Antonio Carlos Rosário | |
dc.date.accessioned | 2013-04-06T22:39:44Z | |
dc.date.available | 2013-04-06T22:39:44Z | |
dc.date.issued | 2005-07 | |
dc.description.abstract | The present work evaluated the epidemiology of human immunodeficiency virus 1/human T-cell lymphotropic virus (HIV-1/HTLV) coinfection in patients living in Belém (state of Pará) and Macapá (state of Amapá), two cities located in the Amazon region of Brazil. A total of 169 blood samples were collected. The sera were tested by enzyme-linked immunosorbent assay to determine the presence of antibodies anti-HTLV-1/2. Confirmation of infection and discrimination of HTLV types and subtypes was performed using a nested polymerase chain reaction targeting the pX and 5' LTR regions, followed by restriction fragment length polymorphism and sequencing analysis. The presence of anti-HTLV1/2 was detected in six patients from Belém. The amplification of the pX region followed by RFLP analysis, demonstrated the presence of HTLV-1 and HTLV-2 infections among two and four patients, respectively. Sequencing HTLV-1 5' LTR indicated that the virus is a member of the Cosmopolitan Group, Transcontinental subgroup. HTLV-2 strains isolated revealed a molecular profile of subtype HTLV-2c. These results are a reflex of the epidemiological features of HIV-1/HTLV-1/2 coinfection in the North region of Brazil, which is distinct from other Brazilian regions, as reported by previous studies. | pt_BR |
dc.identifier.citation | LAURENTINO, R. V., et al. Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 100, n. 4, p. 371-376, jul. 2005. Disponível em: <http://www.scielo.br/pdf/mioc/v100n4/v100n4a06.pdf>. Acesso em: 03 abr. 2013. <http://dx.doi.org/10.1590/S0074-02762005000400006>. | pt_BR |
dc.identifier.issn | 1678-8060 | |
dc.identifier.issn | 0074-0276 | |
dc.identifier.uri | https://repositorio.ufpa.br/handle/2011/3643 | |
dc.language.iso | eng | pt_BR |
dc.rights | Acesso Aberto | |
dc.subject | HIV-1 | pt_BR |
dc.subject | Vírus linfotrópico de células T humanas tipo 1 | pt_BR |
dc.subject | Diagnóstico laboratorial | pt_BR |
dc.subject | Epidemiologia molecular | pt_BR |
dc.subject | Vírus 2 linfotrópico T humano | pt_BR |
dc.subject | Belém - PA | pt_BR |
dc.subject | Macapá - AP | pt_BR |
dc.subject | Pará - Estado | pt_BR |
dc.subject | Amapá - Estado | pt_BR |
dc.subject | Amazônia brasileira | pt_BR |
dc.title | Molecular characterization of human T-cell lymphotropic virus coinfecting human immunodeficiency virus 1 infected patients in the Amazon region of Brazil | pt_BR |
dc.type | Artigo de Periódico |
Arquivo(s)
Pacote Original
1 - 1 de 1
Carregando...
- Nome:
- Artigo_MolecularCharacterizationHuman.pdf
- Tamanho:
- 102.69 KB
- Formato:
- Adobe Portable Document Format
Licença do Pacote
1 - 1 de 1
Nenhuma Miniatura disponível
- Nome:
- license.txt
- Tamanho:
- 1.95 KB
- Formato:
- Item-specific license agreed upon to submission
- Descrição: