Please use this identifier to cite or link to this item: https://repositorio.ufpa.br/jspui/handle/2011/10015
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dc.creatorSILVA, Dorotéa de Fátima Lobato da-
dc.creatorCARDOSO, Jedson Ferreira-
dc.creatorSILVA, Sandro Patroca da-
dc.creatorARRUDA, Leda Mani França de-
dc.creatorMEDEIROS, Renato Lopes Fernandes de-
dc.creatorMORAES, Marluce Matos de-
dc.creatorSOUSA, Rita Catarina Medeiros-
dc.date.accessioned2018-06-13T19:47:40Z-
dc.date.available2018-06-13T19:47:40Z-
dc.date.issued2018-04-
dc.identifier.citationSILVA, Dorotéa de Fátima Lobato da et al. HCMV UL97 phosphotransferase gene mutations may be associated with antiviral resistance in immunocompromised patients in Belém, PA, Northern Brazil. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 2, p. 141-145, mar./abr. 2018. Disponível em: <http://repositorio.ufpa.br/jspui/handle/2011/10015>. Acesso em:.pt_BR
dc.identifier.issn0037-8682pt_BR
dc.identifier.issn1678-9849pt_BR
dc.identifier.urihttp://repositorio.ufpa.br/jspui/handle/2011/10015-
dc.description.abstractHuman cytomegalovirus is one of the causes of opportunist infections in immunocompromised patients, and is triggered by factors such as state of viral latency, weakened immune responses, and development of antiviral resistance to ganciclovir, the only drug offered by the public health system in Brazil to treat the infection. The goal of this study was to identify mutations that may be associated with antiviral resistance in immunocompromised patients. Methods: Molecular analysis was performed in 82 blood samples and subjected to genomic DNA extraction by a silica-based method. Three sequences of the HCMV UL97 gene, which encodes a phosphotransferase protein required for activation of ganciclovir, were amplified by polymerase chain reaction. Pyrosequencing methods were applied to one external 2096-bp segment DNA and two internal sequences between nucleotides 1087 to 1828 to detect mutations in this gene. Results: Approximately 10% of sequences contained mutations between nucleotides 377 and 594, in conserved regions of the UL97 gene, leading to amino acid changes. Eleven coding mutations were identified, including changes leading to amino acid substitutions, E596K and S604F, which were observed in 100% of samples and are described for the first time in Brazil. In addition, one mutation (A594V) that is associated with ganciclovir resistance was detected in a kidney transplant patient. Conclusions: Further studies to detect mutations associated with HCMV resistance to antiviral drugs are required to demonstrate the need to increase the variety and availability of drugs used to treat viral infections in the public health care system in Brazil.pt_BR
dc.description.provenanceSubmitted by Hellen Luz (hellencrisluz@gmail.com) on 2018-06-08T17:32:23Z No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Artigo_HcmvUl97Phosphotransferase.pdf: 980334 bytes, checksum: a15ca8675797152a5732051ea9146922 (MD5)en
dc.description.provenanceApproved for entry into archive by Luciana Alcantara (lalcantara@ufpa.br) on 2018-06-13T19:47:40Z (GMT) No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Artigo_HcmvUl97Phosphotransferase.pdf: 980334 bytes, checksum: a15ca8675797152a5732051ea9146922 (MD5)en
dc.description.provenanceMade available in DSpace on 2018-06-13T19:47:40Z (GMT). No. of bitstreams: 2 license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Artigo_HcmvUl97Phosphotransferase.pdf: 980334 bytes, checksum: a15ca8675797152a5732051ea9146922 (MD5) Previous issue date: 2018-04en
dc.languageengpt_BR
dc.publisherUniversidade Federal do Parápt_BR
dc.relation.ispartofRevista da Sociedade Brasileira de Medicina Tropicalpt_BR
dc.rightsAcesso Abertopt_BR
dc.source.urihttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000200141&lng=pt&nrm=isopt_BR
dc.subjectCitomegalovíruspt_BR
dc.subjectMutagênesept_BR
dc.subjectResistência antiviralpt_BR
dc.subjectMutaçõespt_BR
dc.subjectFosfotransferasept_BR
dc.titleHCMV UL97 phosphotransferase gene mutations may be associated with antiviral resistance in immunocompromised patients in Belém, PA, Northern Brazilpt_BR
dc.typeArtigo de Periódicopt_BR
dc.publisher.countryBrasilpt_BR
dc.publisher.initialsUFPApt_BR
dc.subject.cnpqCNPQ::CIENCIAS DA SAUDE::SAUDE COLETIVA::SAUDE PUBLICApt_BR
dc.creator.Latteshttp://lattes.cnpq.br/5064843368253793pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/6338193279074109pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/0476330416864057pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/5600822689860398pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/0734229589851760pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/9163804242420760pt_BR
dc.creator.Latteshttp://lattes.cnpq.br/3560941703812539pt_BR
dc.citation.volume51pt_BR
dc.citation.issue02pt_BR
dc.citation.spage141pt_BR
dcterms.citation.epage145pt_BR
dc.identifier.doihttp://dx.doi.org/10.1590/0037-8682-0345-2017pt_BR
Appears in Collections:Artigos Científicos - NMT

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