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metadata.dc.type: Artigo de Periódico
Issue Date: Apr-2018
metadata.dc.creator: SILVA, Dorotéa de Fátima Lobato da
CARDOSO, Jedson Ferreira
SILVA, Sandro Patroca da
ARRUDA, Leda Mani França de
MEDEIROS, Renato Lopes Fernandes de
MORAES, Marluce Matos de
SOUSA, Rita Catarina Medeiros
Title: HCMV UL97 phosphotransferase gene mutations may be associated with antiviral resistance in immunocompromised patients in Belém, PA, Northern Brazil
Citation: SILVA, Dorotéa de Fátima Lobato da et al. HCMV UL97 phosphotransferase gene mutations may be associated with antiviral resistance in immunocompromised patients in Belém, PA, Northern Brazil. Revista da Sociedade Brasileira de Medicina Tropical, Uberaba, v. 51, n. 2, p. 141-145, mar./abr. 2018. Disponível em: <>. Acesso em:.
Abstract: Human cytomegalovirus is one of the causes of opportunist infections in immunocompromised patients, and is triggered by factors such as state of viral latency, weakened immune responses, and development of antiviral resistance to ganciclovir, the only drug offered by the public health system in Brazil to treat the infection. The goal of this study was to identify mutations that may be associated with antiviral resistance in immunocompromised patients. Methods: Molecular analysis was performed in 82 blood samples and subjected to genomic DNA extraction by a silica-based method. Three sequences of the HCMV UL97 gene, which encodes a phosphotransferase protein required for activation of ganciclovir, were amplified by polymerase chain reaction. Pyrosequencing methods were applied to one external 2096-bp segment DNA and two internal sequences between nucleotides 1087 to 1828 to detect mutations in this gene. Results: Approximately 10% of sequences contained mutations between nucleotides 377 and 594, in conserved regions of the UL97 gene, leading to amino acid changes. Eleven coding mutations were identified, including changes leading to amino acid substitutions, E596K and S604F, which were observed in 100% of samples and are described for the first time in Brazil. In addition, one mutation (A594V) that is associated with ganciclovir resistance was detected in a kidney transplant patient. Conclusions: Further studies to detect mutations associated with HCMV resistance to antiviral drugs are required to demonstrate the need to increase the variety and availability of drugs used to treat viral infections in the public health care system in Brazil.
Keywords: Citomegalovírus
Resistência antiviral
Series/Report no.: Revista da Sociedade Brasileira de Medicina Tropical
ISSN: 0037-8682
1678-9849 Brasil
Publisher: Universidade Federal do Pará
metadata.dc.publisher.initials: UFPA
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - NMT

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