Use este identificador para citar ou linkar para este item:
https://repositorio.ufpa.br/jspui/handle/2011/10855
Tipo: | Artigo de Periódico |
Data do documento: | Mai-2018 |
Autor(es): | BRITO, Felipe Nunes VENDRAMIN, Fabiel Spani LOPES, Cinthia Távora de Albuquerque COSTA, Mayra Pauline Ribeiro OHASHI, Otavio Mitio MAIA, José Guilherme Soares FERREIRA, Lydia Masako SILVA, Joyce Kelly do Rosário da MIRANDA, Moysés dos Santos |
Afiliação do(s) Autor(es): | BRITO, F. N.; VENDRAMIN, F. S.; LOPES, C. T. A.; COSTA, M. P. R.; OHASHI, O. M.; SILVA, J. K. R.; MIRANDA, M. S. Universidade Federal do Pará |
Título: | Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides |
Citar como: | BRITO, Felipe Nunes et al. Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides. Acta Cirúrgica Brasileira, São Paulo, v. 33, n. 5, p. 431-438, May. 2018. DOI: http://dx.doi.org/10.1590/s0102-865020180050000005. Disponível em: http://repositorio.ufpa.br/jspui/handle/2011/10855. Acesso em:. |
Abstract: | Purpose: To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells. Methods: Stem cells were isolated from human adipose tissue by liposuction. After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/mL, 5.0 μg/mL, and 25.0 μg/mL). Cells were analyzed by the MTT assay at passage 1 (P1), and cell proliferation of control and 1 μg/mL groups was determined with a hemocytometer at P2 and P3. Results: Viability of the essential oil-treated cells was significantly higher than the control group at P1 (p = 0.0008). The treatment with the oil, at a concentration of 1 µg/mL, led to increases of 24.8% at P1 and 43.0% at P3 in the rate of cell proliferation compared with control cells. Conclusion: Supplementing culture medium with essential oil of Lippia origanoides increased cell proliferation, especially at later passages. |
Palavras-chave: | Stem cells Adipose tissue Cell proliferation Thymol |
Título do Periódico: | Acta Cirúrgica Brasileira |
ISSN: | 1678-2674 |
País: | Brasil |
Instituição: | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
Sigla da Instituição: | SOBRADPEC |
Tipo de Acesso: | Acesso Aberto |
Fonte URI: | http://ref.scielo.org/gjkp4n |
Identificador DOI: | http://dx.doi.org/10.1590/s0102-865020180050000005 |
Aparece nas coleções: | Artigos Científicos - FAMED/ICS |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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Article_ProliferationHumanAdipose.pdf | INGLÊS | 1,15 MB | Adobe PDF | Visualizar/Abrir |
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