Modelo experimental para indução de hemiparkinsonismo por 6-hidroxidopamina em primatas sapajus apella e avaliação das alterações motoras

Abstract

Parkinson's disease is currently the second most common neurodegenerative disease in the world, with a high incidence in North and South America and Europe, for more than 50 years we have not seen any revolutionary treatment for the disease and many aspects of its neuropathology that still remain without a concrete enlightenment, in this feeling the experimental model in primates approaches the human reality are invaluable value for the development of new therapies and elucidation on mechanisms related to the disease. The 6-hydroxydopamine model in primates is a model that mimics some motor symptoms characteristic of PD. The present study aimed to develop a protocol for the induction of HemiParkinsonism in Sapajus apella primates. Three Sapajus Apella monkeys, all adult males, were submitted to daily conditioning sessions using the positive reinforcement clicker technique for primate chair positioning. Concurrently, the staircase and Brinkman tray motor tests were performed to determine laterality by the manual preference and dominance attributes. After this period, two 6-OHDA induction protocols were performed, the first protocol was injected into 10 sites in the nucleus striatum and the second protocol was injected into 10 sites in the nigrostriatal pathways, one week after the injections were performed twelve weeks of clinical analysis . All animals learned the input and positioning behaviors in the chair in a minimum of 30 sessions using pure positive reinforcement. The results of the staircase test demonstrated that the animals presented laterality consistent with the assignments of manual preference and dominance. The Brinkman test, specifically, presented lower sensitivity for determination of the same attributes, despite being the most commonly used test. Clinical analysis revealed that the second induction protocol had more motor symptoms characteristic of PD. Induction by 6-OHDA in the nigrostriatal pathways has been shown to be a good induction method for treatment studies and for a better understanding of the disease.