Use este identificador para citar ou linkar para este item: https://repositorio.ufpa.br/jspui/handle/2011/15618
Tipo: Artigo de Periódico
Data do documento: 2012
Autor(es): SANTOS, Anderson Rodrigues dos
CARNEIRO, Adriana Ribeiro
GALA-GARCÍA, Alfonso
GOMIDE, Anne Cybelle Pinto
BARH, Debmalya
BARBOSA, Eudes Guilherme Vieira
ABURJAILE, Flavia Figueira
DORELLA, Fernanda Alves
ROCHA, Flávia de Souza
GUIMARÃES, Luís Carlos
TURK, Meritxell Zurita
RAMOS, Rommel Thiago Juca
ALMEIDA, Sintia Silva de
SOARES, Siomar de Castro
PEREIRA, Ulisses de Pádua
ABREU, Vinicius Augusto Carvalho de
SILVA, Artur Luiz da Costa da
MIYOSHI, Anderson
AZEVEDO, Vasco Ariston de Carvalho
Afiliação do(s) Autor(es): CARNEIRO, A. R.; RAMOS, R. T. J.; SILVA, A. L. C. Universidade Federal do Pará
Título: The Corynebacterium pseudotuberculosis in silico predicted pan-exoproteome
Citar como: ANDERSON, R. Santos et al. The Corynebacterium pseudotuberculosis in silico predicted pan-exoproteome. BMC Genomics, online, v. 13, n. S6, p. 1-12, 2012. Supl. 5. DOI: https://doi.org/10.1186/1471-2164-13-S5-S6. Disponível em: http://repositorio.ufpa.br/jspui/handle/2011/15618. Acesso em:.
Abstract: Background: Pan-genomic studies aim, for instance, at defining the core, dispensable and unique genes within a species. A pan-genomics study for vaccine design tries to assess the best candidates for a vaccine against a specific pathogen. In this context, rather than studying genes predicted to be exported in a single genome, with pan genomics it is possible to study genes present in different strains within the same species, such as virulence factors. The target organism of this pan-genomic work here presented is Corynebacterium pseudotuberculosis, the etiologic agent of caseous lymphadenitis (CLA) in goat and sheep, which causes significant economic losses in those herds around the world. Currently, only a few antigens against CLA are known as being the basis of commercial and still ineffective vaccines. In this regard, the here presented work analyses, in silico, five C. pseudotuberculosis genomes and gathers data to predict common exported proteins in all five genomes. These candidates were also compared to two recent C. pseudotuberculosis in vitro exoproteome results. Results: The complete genome of five C. pseudotuberculosis strains (1002, C231, I19, FRC41 and PAT10) were submitted to pan-genomics analysis, yielding 306, 59 and 12 gene sets, respectively, representing the core, dispensable and unique in silico predicted exported pan-genomes. These sets bear 150 genes classified as secreted (SEC) and 227 as potentially surface exposed (PSE). Our findings suggest that the main C. pseudotuberculosis in vitro exoproteome could be greater, appended by a fraction of the 35 proteins formerly predicted as making part of the variant in vitro exoproteome. These genomes were manually curated for correct methionine initiation and redeposited with a total of 1885 homogenized genes. Conclusions: The in silico prediction of exported proteins has allowed to define a list of putative vaccine candidate genes present in all five complete C. pseudotuberculosis genomes. Moreover, it has also been possible to define the in silico predicted dispensable and unique C. pseudotuberculosis exported proteins. These results provide in silico evidence to further guide experiments in the areas of vaccines, diagnosis and drugs. The work here presented is the first whole C. pseudotuberculosis in silico predicted pan-exoproteome completed till today.
Palavras-chave: Corynebacterium pseudotuberculosis
Título do Periódico: BMC Genomics
ISSN: 1471-2164
País: Reino Unido
Instituição: BioMed Central Ltd
Tipo de Acesso: Acesso Aberto
Fonte URI: https://bmcgenomics.biomedcentral.com/articles/10.1186/1471-2164-13-S5-S6
Identificador DOI: doi.org/10.1186/1471-2164-13-S5-S6
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