Use este identificador para citar ou linkar para este item: https://repositorio.ufpa.br/jspui/handle/2011/15726
Tipo: Artigo de Periódico
Data do documento: 2017
Autor(es): ALMEIDA, Sintia Silva de
DORNELES, Elaine Maria Seles
DINIZ, Carlos Augusto Almeida
ABREU, Vinicius Augusto Carvalho de
SOUSA, Cassiana Severiano de
ALVES, Jorianne Thyeska Castro
CARNEIRO, Adriana Ribeiro
VILAS BOAS, Priscilla Carolinne Bagano
SPIER, Sharon
BARH, Debmalya
LAGE, Andrey Pereira
FIGUEIREDO, Henrique Cesar Pereira
AZEVEDO, Vasco Ariston de Carvalho
Afiliação do(s) Autor(es): ALVES, J. T. C.; CARNEIRO, A. R. Universidade Federal do Pará
Título: Quadruplex PCR assay for identification of Corynebacterium pseudotuberculosis differentiating biovar Ovis and Equi
Agência de fomento: 
Citar como: ALMEIDA, Sintia et al. Quadruplex PCR assay for identification of Corynebacterium pseudotuberculosis differentiating biovar Ovis and Equi. BMC Genomics, online, v. 13, n. 390, p. 1-8, 2017. DOI: https://doi.org/10.1186/s12917-017-1210-5. Disponível em: https://repositorio.ufpa.br/jspui/handle/2011/15726. Acesso em:.
Abstract: Background: Corynebacterium pseudotuberculosis is classified into two biovars, nitrate-negative biovar Ovis which is the etiologic agent of caseous lymphadenitis in small ruminants and nitrate-positive biovar Equi, which causes abscesses and ulcerative lymphangitis in equines. The aim of this study was to develop a quadruplex PCR assay that would allow simultaneous detection and biovar-typing of C. pseudotuberculosis. Methods: In the present study, genomes of C. pseudotuberculosis strains were used to identify the genes involved in the nitrate reduction pathway to improve a species identification three-primer multiplex PCR assay. The nitrate reductase gene (narG) was included in the PCR assay along with the 16S, rpoB and pld genes to enhance the diagnosis of the multiplex PCR at biovar level. Results: A novel quadruplex PCR assay for C. pseudotuberculosis species and biovar identification was developed. The results of the quadruplex PCR of 348 strains, 346 previously well-characterized clinical isolates of C. pseudotuberculosis from different hosts (goats, sheep, horse, cattle, buffalo, llamas and humans), the vaccine strain 1002 and the type strain ATCC 19410T , were compared to the results of nitrate reductase identification by biochemical test. The McNemar’s Chi-squared test used to compare the two methods used for C. pseudotuberculosis biovar identification showed no significant difference (P = 0.75) [95% CI for odds ratio (0.16–6.14)] between the quadruplex PCR and the nitrate biochemical test. Concordant results were observed for 97.13% (338 / 348) of the tested strains and the kappa value was 0.94 [95% CI (0.90–0.98)]. Conclusions: The ability of the quadruplex assay to discriminate between C. pseudotuberculosis biovar Ovis and Equi strains enhances its usefulness in the clinical microbiology laboratory.
Palavras-chave: Caseous lymphadenitis
Diagnosis
Nitrate reductase
Horse
Sheep
Goats
Título do Periódico: BMC Genomics
ISSN: 1471-2164
País: Reino Unido
Instituição: BioMed Central Ltd
Tipo de Acesso: Acesso Aberto
Attribution-NonCommercial-NoDerivs 3.0 Brazil
Fonte URI: https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1210-5#Ack1
Identificador DOI: 10.1186/s12917-017-1210-5
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