Use este identificador para citar ou linkar para este item: https://repositorio.ufpa.br/jspui/handle/2011/15742
Tipo: Artigo de Periódico
Data do documento: 2020
Autor(es): POPIN, Rafael Vicentini
DELBAJE, Endrews
ABREU, Vinicius Augusto Carvalho de
RIGONATO, Janaina
DÖRR, Felipe Augusto
PINTO JUNIOR, Ernani
SIVONEN, Kaarina
FIORE, Marli de Fátima
Afiliação do(s) Autor(es): ABREU, V. A. C. Universidade Federal do Pará
Título: Genomic and Metabolomic Analyses of Natural Products in Nodularia spumigena Isolated from a Shrimp Culture Pond
Citar como: POPIN, Rafael Vicentini. Genomic and Metabolomic Analyses of Natural Products in Nodularia spumigena Isolated from a Shrimp Culture Pond. Toxins, online, v. 12, n. 141, p. 1-20, 2020. DOI: https://doi.org/10.3390/toxins12030141. Disponível em: http://repositorio.ufpa.br/jspui/handle/2011/15742. Acesso em:.
Abstract: The bloom-forming cyanobacterium Nodularia spumigena CENA596 encodes the biosynthetic gene clusters (BGCs) of the known natural products nodularins, spumigins, anabaenopeptins/namalides, aeruginosins, mycosporin-like amino acids, and scytonemin, along with the terpenoid geosmin. Targeted metabolomics confirmed the production of these metabolic compounds, except for the alkaloid scytonemin. Genome mining of N. spumigena CENA596 and its three closely related Nodularia strains—two planktonic strains from the Baltic Sea and one benthic strain from Japanese marine sediment—revealed that the number of BGCs in planktonic strains was higher than in benthic one. Geosmin—a volatile compound with unpleasant taste and odor—was unique to the Brazilian strain CENA596. Automatic annotation of the genomes using subsystems technology revealed a related number of coding sequences and functional roles. Orthologs from the Nodularia genomes are involved in the primary and secondary metabolisms. Phylogenomic analysis of N. spumigena CENA596 based on 120 conserved protein sequences positioned this strain close to the Baltic Nodularia. Phylogeny of the 16S rRNA genes separated the Brazilian CENA596 strain from those of the Baltic Sea, despite their high sequence identities (99% identity, 100% coverage). The comparative analysis among planktic Nodularia strains showed that their genomes were considerably similar despite their geographically distant origin.
Palavras-chave: Cyanobacteria
Gene cluster
Mass spectrometry
Cyanotoxins
Terpenes
Water quality
Título do Periódico: Toxins
ISSN: 2072-6651
País: Suica
Instituição: Multidisciplinary Digital Publishing Institute
Sigla da Instituição: MDPI
Tipo de Acesso: Acesso Aberto
Fonte URI: https://www.mdpi.com/2072-6651/12/3/141
Identificador DOI: 10.3390/toxins12030141
Aparece nas coleções:Artigos Científicos - ICB

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