Physalis angulata estimula proliferação de células-tronco neurais do giro denteado hipocampal de camundongos adultos

Abstract

Aim: Newborn neurons emerge from neural stem cells (NSCs) from niches in the mammalian adult brain. These cells are incorporated into functional circuits and may be important to acquisition and retention of memory. Therefore, the search for new compounds that enhance proliferation and differentiation of neural stem cells in the hippocampus represent a significant scientific challenge with great promise. Methods and results: We have used aqueous extract from of the Physalis angulata on the neurogenesis in the subgranular zone of hipocampal dentate gyrus of adult mice using 5`-bromo-2`-deoxyuridine (BrdU)-pulse chase method. Increased doses (0.1; 1; 5mg/Kg) of Physalis angulata were given to adult male BALB/c mice with 6 to 8-weeks-old; or 0.9% NaCl (control). Mice were sacrificed at 24 hours or 7 days after the BrdU administration, and hippocampal slices were processed for immunohistochemistry. We found that Physalis angulata did not modify the mice behavior at any dose used, but increased the number of BrdU-positive cells in the subgranule zone of hipocampal dentate gyrus 24 hours or 7 days after injection. Physalis angulata not showed BrdU-positive cells out subgranule cell layer (ectopic neurogenesis). All procedures involving animal care and experimentation were performed in accordance with the guidelines of the Ethical Committee for Research with Experimental Animals of the Universidade Federal do Pára (BIO058-12). Conclusion: These results suggest that SM2 could be stimulating the proliferation of neural stem cells in hipocampal dentate gyrus, and also sustain the hipocampal network because increases the BrdU-positive cells in differentiation process in the sub granular zone of hippocampus of adult mice.