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metadata.dc.type: Artigo de Periódico
Issue Date: Dec-2013
metadata.dc.creator: ONDEI, Luciana de Souza
ESTEVÃO, Isabeth da Fonseca
ROCHA, Marina Ibelli Pereira
SOUZA, Dorotéia Rossi Silva
PINHEL, Marcela Augusta de Souza
DOMINGOS, Claudia Regina Bonini
Title: Oxidative stress and antioxidant status in beta-thalassemia heterozygotes
Citation: ONDEI, Luciana de Souza et al. Oxidative stress and antioxidant status in beta-thalassemia heterozygotes. Revista Brasileira de Hematologia e Hemoterapia, São José do Rio Preto, v. 35, n. 6, p. 409-413, nov./dez. 2013. Disponível em: <>. Acesso em: 27 nov. 2014. <>.
Abstract: Background: Several studies have evaluated the oxidant and antioxidant status of thalassemia patients but most focused mainly on the severe and intermediate states of the disease. Moreover, the oxidative status has not been evaluated for the different beta-thalassemia mutations. Objective: To evaluate lipid peroxidation and Trolox equivalent antioxidant capacity in relation to serum iron and ferritin in beta thalassemia resulting from two different mutations (CD39 and IVS-I-110) compared to individuals without beta-thalassemia. Methods: One hundred and thirty subjects were studied, including 49 who were heterozygous for beta-thalassemia and 81 controls. Blood samples were subjected to screening tests for hemoglobin. Allele-specific polymerase chain reaction was used to confirm mutations for beta-thalassemia, an analysis of thiobarbituric acid reactive species was used to determine lipid peroxidation, and Trolox equivalent antioxidant capacity evaluations were performed. The heterozygous beta-thalassemia group was also evaluated for serum iron and ferritin status. Results: Thiobarbituric acid reactive species (486.24 ± 119.64 ng/mL) and Trolox equivalent antioxidant capacity values (2.23 ± 0.11 mM/L) were higher in beta-thalassemia heterozygotes compared to controls (260.86 ± 92.40 ng/mL and 2.12 ± 0.10 mM/L, respectively; p-value < 0.01). Increased thiobarbituric acid reactive species values were observed in subjects with the CD39 mutation compared with those with the IVS-I-110 mutation (529.94 ± 115.60 ng/mL and 453.39 ± 121.10 ng/mL, respectively; p-value = 0.04). However, average Trolox equivalent antioxidant capacity values were similar for both mutations (2.20 ± 0.08 mM/L and 2.23 ± 0.12 mM/L, respectively; p-value = 0.39). There was no influence of serum iron and ferritin levels on thiobarbituric acid reactive species and Trolox equivalent antioxidant capacity values. Conclusion: This study shows an increase of oxidative stress and antioxidant capacity in beta-thalassemia heterozygotes, mainly in carriers of the CD39 mutation.
Keywords: Estresse oxidativo
Talassemia beta
Beta globina
Peróxidos lipídicos
Substância reactiva ao ácido tiobarbitúrico
ISSN: 1516-8484
metadata.dc.rights: Acesso Aberto
Appears in Collections:Artigos Científicos - ICB

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