Artigos Científicos - CCAST

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Acesso aberto (Open Access)
Relative age effect on the reaction time of soccer players under 13 years old
(Universidade Estadual Paulista, 2015-06) PENNA, Eduardo Macedo; MELLO, Marco Túlio de; FERREIRA, Renato Melo; MORAES, Luiz Carlos Couto de Albuquerque; COSTA, Varley Teoldo da
The relative age effect, which is the advantage obtained by an athlete born closer to the beginning of the selection year, has been shown to be an important variable in the development of soccer players. This study aimed to evaluate the influence of the season of birth on the choice reaction time (CRT) of elite soccer athletes in the under 13 years of age category. Seventy-six athletes participated who were 13.36 ± 0.45 years old. The volunteers were tested with a CRT test, and the results were divided according to the semester of birth (S1 or S2). We conducted a t-test in order to compare the results between groups and a Pearson correlation analysis to verify the associations between variables. The results indicated no differences in reaction time (r= .033 and p = .772) or movement time (r = .0073 and p = .530). It was concluded that for this population, the season of birth does not influence the performance of the capabilities tested.
Acesso aberto (Open Access)
Relative age effect and dropout causes in a multisport club setting. Is there a special reason to give up?
(Universidade Estadual Paulista, 2018) PENNA, Eduardo Macedo; CAMPOS, Bruno Teobaldo; GONÇALVES, Gabriela Gomes Pavan; GODINHO, Gustavo Henrique Pereira; LIMA, Claudio Olívio Vilela; PRADO, Luciano Sales
The aim of the present study was 1) compare the drop-out rates between athletes born in different trimesters of the competitive year from different sports and 2) identify the causes pointed by the dropped-out athletes born in different selection year trimesters to disengage from the competitive sports environment. Methods: Data from 966 athletes who left the competitive sports context were analyzed under the Relative Age Effect (RAE) theoretical framework during 3 consecutive seasons. The drop-out rate and the reason to quit the competitive environment were analyzed by a routine club questionnaire. Results: An expected reverse RAE on drop-out rates was identified, in which the early born athletes were overrepresented (p<0.001). Regarding the reasons to disengage from competitive sports environment, no reason emerged as the main cause to dropout between athletes born in different selection year trimester. Conclusion: It was concluded that despite the common assumption that personal psychological issues related to RAE in late born athletes are important factors influencing sports disengagement, the relationship between drop-out and RAE is more complex and multifactorial.
Acesso aberto (Open Access)
Desenvolvimento de habilidades cognitivas e ensino de matemática em um Clube de Ciências da Amazônia
(Universidade Federal do Pará, 2019-06) COELHO, Antonia Ediele de Freitas; ALMEIDA, Willa Nayana Corrêa; MALHEIRO, João Manoel da Silva
This research sought to analyze the Levels of Cognitive Abilities developed and manifested by a group of seven students participating in the Science “Prof. Club Dr. Cristovam W. P. Diniz”, during an Investigative Teaching Sequence on concepts of plane geometry. We also want to ponder the questions elaborated by the teacher-monitor and their intrinsic relationship with the abilities manifested by the group of students. We characterize this research as qualitative, using Content Analysis to interpretation of the speeches between the teacher-monitor and the students participating in the club. The space investigated is considered an alternative environment destined to the teaching, research and extension of didactic actions directed to Sciences and Mathematics. The applied experimental activity was entitled "Problem of Forms" and sought to discuss the introductory concepts of area and perimeter of plane figures, exploring issues related to the maximization of areas and minimization of perimeters. During the analysis, we identified that the abilities developed by the students in their discourses are continuously related to the proposed, being possible to identify Levels that varied between Algorithmics and Higher Order Cognitive Skills, defined between N1 and N5 of Cognitive Skills. Moreover, the questions developed by the teacher-monitor included Levels that developed according to the dynamics of established relationships, passing between P1 and P4.
Acesso aberto (Open Access)
The relative age effect on brazilian elite volleyball
(Universidade Federal do Pará, 2018-10) PARMA, Juliana Otoni; PENNA, Eduardo Macedo
The relative age effect (RAE) refers to the advantage of the athletes born on the first months of the sport selection period compared to those born months later during such a selection process, which tends to favor athletes who are in a more developed maturational stage or those who have more advanced physical development compared to their younger counterparts born in the same year. This advantage occurs mainly in sport whose physical characteristics are determinant for obtaining success, such as volleyball, which leads to the premature exclusion or dropout of potential talents from sport. This study aims at assessing the RAE presence in Brazilian men’s and women’s elite volleyball by analysing the number of athletes who were born in each trimester of the year and had been ranked to the main national Super Leagues. The results showed no effect related to the RAE in Brazilian women’s elite volleyball, whereas there was a higher representation of male athletes born in the first trimester compared to the ones born on the 3rd and 4th trimesters. These results point to a need for better understanding the causes and consequences of RAE in Brazilian men’s volleyball in order to avoid premature exclusion or dropout of potential talents, thus, enabling an even greater development of this sport in the country.
Acesso aberto (Open Access)
Ocorrência de Cryptosporidium spp. em bezerros bubalinos no estado do Pará
(Universidade Federal do Pará, 2014-12) DESTRO, Karen Cristiane; VIANA, Rinaldo Batista; BENIGNO, Raimundo Nonato Moraes; CHAVES, Luciara Celi da Silva; PEREIRA, Washington Luiz Assunção
Cryptosporidiosis is a zoonosis caused by opportunistic protozoa of the genus Cryptosporidium spp. that attacks various animal species, including humans. This study had the objective of determining the occurrence of infection by Cryptosporidium spp. in buffalo raised in the state of Pará, Brazil, and establishing the relations between Cryptosporidium spp., the age of the animal and the presence of clinical signs of diarrhea. A total of 253 samples of feces from one day to 6-month old buffalo were analyzed. The fecal samples, collected directly from the rectum, were classified as diarrheal and non-diarrheal and analyzed with an optical microscope after centrifuge-concentration and staining with Kinyoun stain. Laboratory analysis revealed the presence of Cryptosporidium spp. in 2.37% (6/253) samples, all of which had a firm consistency. No significant relation was established between infection by Cryptosporidium spp., the animals age from one day to 6-month and clinical symptoms of diarrhea.
Acesso aberto (Open Access)
Long-term assessment of a modified tibial tuberosity advancement technique in dogs
(FEPMVZ Editora, 2018-08) MEDEIROS, Regina Mendes; SILVA, Marco Augusto Machado; TEIXEIRA, Pedro Paulo Maia; CHUNG, Denise Granato; CONCEIÇÃO, Maria Eduarda Bastos Andrade Moutinho da; CHIERICE, Gilberto Orivaldo; PADILHA FILHO, João Guilherme; DIAS, Luís Gustavo Gosuen Gonçalves
The purpose of study was to assess long-term clinical and radiographic aspects of dogs’ stifle joints which had undergone a modified tibial tuberosity advancement technique (mTTA). A total of 15 stifles that had undergone mTTA for CCL disease of 11 patients were included in this study. Assessments involved patient’s gait analysis, cranial drawer and tibial compression tests, stifle goniometry range of articular motion, thigh and leg girth and radiographic evidence of progression of osteoarthrosis. Variables were compared between operated and healthy limbs and among moments (M0) on the early postop; (M1) 120 days postop; and (M2) approximately 5 years following surgery. A questionnaire regarding owner’s perceptions after approximately 5 years of surgery was assessed. Most dogs presented positive response to cranial drawer and tibial compression tests on operated knees. There was also decrease on goniometry and thigh girth and increase in leg girth. Radiographic evidence of progression of osteoarthritis was seen especially on the long-term follow-up (M2). On gait analysis, most animals presented some degree of lameness in different conditions, in contrast to owners’ perceptions. Osteoarthritis still develops in dogs following mTTA surgery for CCL disease. However, owners were overall satisfied with their recovery and would be willing to accept indication of mTTA for dogs with ruptured CCL.
Acesso aberto (Open Access)
Indução do estro em cutias (Dasyprocta leporina) utilizando-se protocolos à base de prostaglandina isolada ou em associação com análogo de GnRH
(FEPMVZ Editora, 2018-06) PEIXOTO, Gislayne Christianne Xavier; MAIA, Keilla Moreira; CAMPOS, Lívia Batista; OLIVEIRA, Gislaine Borba; OLIVEIRA, Maurilio Fernandes de; BRITO, Adriel Behn de; DOMINGUES, Sheyla Farhayldes Souza; SILVA, Alexandre Rodrigues; ALMEIDA, Laressa Marques de
We compared the efficiency of protocols for estrus induction in agoutis. Five females received double intraperitoneal administration of cloprostenol (5µg) on a 2-days interval; other five females were treated with intravulvar administration of 30µg gonadotrophin release hormone analogue (GnRH associated to intraperitoneal administration of 5µg cloprostenol after seven days and a new administration of GnRH analogue after two days. Every 3 days, the agoutis’ reproductive cycle was monitored by blood collection for hormonal analysis, ovarian ultrasound and vaginal cytology. Two females, originally in luteal phase, that received isolated prostaglandin presented estrous signs at 3 and 6 days after the second drug administration. From the females that received the association, two that were originally in luteal phase presented estrus at 4 days after treatment, and one other presented estrus only after 10 days. There was no significant statistical difference regarding the efficiency of treatments for estrus induction (P>0.05). We conclude that, according to the protocols tested in the study, the use of isolated prostaglandin or its association to GnRH analogue for estrus induction in D. leporine shows an efficiency limited to the females that were in luteal phase in the beginning of the treatment.
Acesso aberto (Open Access)
Behavior of buffalo heifers reared in shaded and unshaded pastures during the dry season on Marajó Island, Pará, Brazil
(Universidade Federal do Pará, 2019-02-25) ALMEIDA, Jean Caio Figueiredo de; JOSET, Waléria Cristina Lopes; BARBOSA, Antônio Vinícius Corrêa; LOURENÇO JÚNIOR, José de Brito; SILVA, Jamile Andréa Rodrigues da; NORONHA, Rafaela de Paula Pachêco
The objective of this research was to evaluate the behavior of twenty buffalo heifers reared during the dry season on Marajó Island, Pará, Brazil. The animals were distributed into two groups, those with shade (WS) and those without shade (WOS). The experimental day was divided into six shifts: morning (6:00 to 9:55 a.m.), intermediate (10:00 a.m. to 1:55 p.m.), afternoon (2:00 to 5:55 p.m.), evening (6:00 to 9:55 p.m.), night (10:00 p.m. to 1:55 a.m.), and early morning (2:00 to 5:55 a.m.). The WS group was kept in silvipastoral system paddocks, while the WOS group was kept in unshaded paddocks. Climatic data were registered and temperature and humidity index (THI) were calculated. Behavior data such as grazing, rumination, idle time, and other activities (walking, defecating, mounting, drinking water, urinating, eating salt) were evaluated. The results showed that the THI was higher in the WOS group. Grazing time was higher in the WOS group than in the WS group in the intermediate shift (p < 0.05), while in the afternoon, evening, and early morning shifts, the WS group spent more time grazing than the WOS group. In the intermediate and early morning shifts, the WS group spent more time ruminating (p < 0.05); the WS group ruminated more than the WOS group. The WOS group had more idle time, especially in the evening and early morning shifts (p < 0.05), while the WS group had more idle time in the intermediate shift (p < 0.05). We conclude that buffaloes graze and ruminate more intensely when they are reared in a shaded system on the island of Marajó
Acesso aberto (Open Access)
Thermoregulatory responses of female buffaloes reared under direct sunlight and shaded areas in the dry season on Marajó Island, Brazil
(Universidade Federal do Pará, 2018-08-16) JOSET, Waléria Cristina Lopes; SILVA, Jamile Andréa Rodrigues da; GODINHO, Letícia Athaíde; BARBOSA, Antônio Vinícius Corrêa; MARTORANO, Lucieta Guerreiro; LOURENÇO JÚNIOR, José de Brito
This research aimed to assess the effects of dry season climate variables on Marajó Island, Brazil, on the thermoregulatory responses of twenty female buffaloes, assigned to shaded (SD), and unshaded groups (US). Data on air temperature (AT), relative air humidity, wind velocity (WV), respiratory rate (RR), rectal temperature (RT), and body surface temperature (BST) were recorded at 6 a.m., 10 a.m., 2 p.m., 6 p.m., and 10 p.m. The temperature and humidity index (THI), practical buffalo comfort climatic conditions index (BCCCIp), and practical buffalo environmental comfort index (BECIp) were calculated. At 10 a.m. and 2 p.m., WV was the highest. The RR of animals in both treatments differed only at 10 a.m. and 2 p.m. A difference in RT between treatments was seen at 10 a.m., 2 a.m., and 6 p.m. Significant changes were seen in the BST of animals in both groups at 10 a.m., 2 p.m., and 10 p.m. BECIp was different between the groups at 10 a.m. and 2 p.m., with higher values for the US group. Buffaloes are prone to thermal stress, particularly between 10 a.m. and 2 p.m., when the highest values of AT, THI, and BCCCIp was observed.
Acesso aberto (Open Access)
Aspectos anatomopatológicos em cães naturalmente infectados por Hepatozoon canis
(Universidade Federal do Pará, 2017-02) LIMA, Pâmela Aparecida de; BARÇANTE, Joziana Muniz de Paiva; BOELONI, Jankerle Neves; BEZERRA JÚNIOR, Pedro Soares; WOUTERS, Flademir; WOUTERS, Angelica Terezinha Barth; VARASCHIN, Mary Suzan; SEIXAS, Josilene Nascimento
Canine hepatozoonosis is mainly caused by protozoa Hepatozoon canis and H. americanum that are transmitted by ingestion of infected ticks. Clinical signs may be unspecific or difficult to identify, because usually hepatozoonosis occurs associated with other disease. In Brazil, the parasite and the disease, have been identified in several states, however little is known about the clinical and anatomopathological lesions resulting from the infection. This paper reports five cases of natural infection by Hepatozoon canis in dogs from Minas Gerais State and describes for the first time in Brazil the necropsy and histopathological findings related to infection. Meronts of Hepatozoon sp., submitted to morphometric evaluation, were observed in histological sections of liver, spleen, bone marrow and kidney.
Acesso aberto (Open Access)
Brucellosis in water buffaloes
(Universidade Federal do Pará, 2017-03) SOUSA, Melina Garcia Saraiva de; SALVARANI, Felipe Masiero; BOMJARDIM, Henrique dos Anjos; BRITO, Marilene de Farias; BARBOSA NETO, José Diomedes
The domestication of water buffaloes (Bubalus bubalis) originated in India and China and spread throughout the world and represents an important source of food of high biological value. Given the importance and relevance of brucellosis for buffalo production, this article reviews the history, etiopathogenesis, epidemiology, clinical signs, anatomopathological findings, diagnosis and control of the disease, focusing on data from studies on water buffaloes performed in different countries and the Brazilian Amazon biome.
Acesso aberto (Open Access)
The development of an enzyme-linked immunosorbent assay for Trypanosoma vivax antibodies and its use in epidemiological surveys
(Universidade Federal do Pará, 2006-11) MADRUGA, Cláudio Roberto; ARAÚJO, Flábio Ribeiro de; CAVALCANTE, Gustavo Góes; MARTINS, Charles Ferreira; BARBOSA, Imke Barbara Pfeifer; RIBEIRO, Laura Rios; KESSLER, Raul Henrique; SOARES, Cleber Oliveira; MIGUITA, Midori; MELO, Elaine Silva de Pádua; ALMEIDA, Robson Ferreira Cavalcante de; LIMA JUNIOR, Manoel Sebastião da Costa
There are data indicating that the distribution of Trypanosoma vivax in the Brazilian territory is expanding with potential to reach other areas, where the vectors are present. The detection of anti-trypanosomal antibodies in serum provides important information of the trypanosomal status in cattle herds. For this reason, an enzyme-linked immunosorbent assay (Tv-ELISA-Ab) with crude antigen from one Brazilian isolate of T. vivax was developed and evaluated. The sensitivity and specificity were respectively 97.6 and 96.9%. In the evaluation of cross-reactions, three calves inoculated with T. evansi trypimastigotes blood forms showed optical densities (OD) under the cut-off during the whole experimental period, except one at 45 days post-inoculation. With relation to Babesia bovis, B. bigemina, and Anaplasma marginale, which are endemic hemoparasites in the studied area, the cross-reactions were shown to be 5.7, 5.3, and 1.1%, respectively. The first serological survey of Pantanal and state of Pará showed that T. vivax is widespread, although regions within both areas had significantly different prevalences. Therefore, this Tv-ELISA-Ab may be a more appropriate test for epidemiological studies in developing countries because the diagnostic laboratories in most countries may be able to perform an ELISA, which is not true for polymerase chain reaction.
Acesso aberto (Open Access)
Reliable Energy-Efficient Multilayer Mechanism with Realistic Battery Model and QoE Support in Wireless MANETs
(Universidade Federal do Pará, 2016-03) CARVALHO, Tássio Costa de; FERREIRA JUNIOR, José Jailton Henrique; FRANCÊS, Carlos Renato Lisboa
Evolution in the telecommunications networks and the rapid advances made in wireless mobile communication networks, have taken computer networks to a new communication level. They now provide support for new mobile multimedia applications and are thus fostering the development of new network types and configurations. MANET is an important configuration of network for operations that employ mechanisms to enable dynamic self-configuration, self-optimization and self-healing, which is a challenging task for the routing protocols, new multimedia application and the battery energy consumption of wireless and mobile devices. These protocols are challenging because battery problems are more serious in this type of network and the existing protocols are not adapted to current problems, particularly mobile multimedia streaming, that can be found in most Internet flow. This paper proposes a reliable energy-efficient multilayer mechanism to address this to make improvements in the main current protocols. The proposal adds decision metrics to an energy-efficient computational intelligence technique based on Fuzzy-System with QoE, mobility and battery guarantees, choosing the best path in an efficient and accuracy way. The proposal has been evaluated in a network simulator.
Acesso aberto (Open Access)
Contos de Grimm e novos contos de Grimm: tradução e adaptação em Monteiro Lobato
(Universidade Federal do Pará, 2016-04) TRUSEN, Sylvia Maria
Monteiro Lobato’s work is well known in the field of literature for children, and so is his effort to emancipate the genre of European canons. However, his task as translator to transfer and circulate a large number of foreign literature works within the national environment, seems to contradict his project of founding a own Brazilian children’s literature. With the review of the reception of the Grimm´s collection in his work, especially the adaptations of Grimm Fairy Tales and the New Tales of Grimm, the task is therefore to analyse how Lobato has reconciled the purposes in apparently disagree.
Acesso aberto (Open Access)
Classificação de estratos florestais utilizando redes neurais artificiais e dados de sensoriamento remoto
(Instituto de Pesquisas Ambientais em Bacias Hidrográficas, 2016-09) GONÇALVES, Wanderson Gonçalves e; RIBEIRO, Hebe Morganne Campos; SÁ, José Alberto Silva de; MORALES, Gundisalvo Piratoba; FERREIRA FILHO, Hélio Raymundo; ALMEIDA, Arthur da Costa
This study classified forest types using neural network data from a forest inventory provided by the "Florestal e da Biodiversidade do Estado do Pará" (IDEFLOR-BIO), and Bands 3, 4 and 5 of TM from the Landsat satellite. The information from the satellite images was extracted using QGIS software 2.8.1 Wien and was used as a database for training neural networks belonging to the software tools package MATLAB(r) R2011b. The neural networks were trained to classify two forest types: Rain Forest of Lowland Emerging Canopy (Dbe) and Rain Forest of Lowland Emerging Canopy plus Open with palm trees (Dbe + Abp) in the Mamuru Arapiuns glebes of Pará State, and were evaluated in terms of the indicators confusion matrix, overall accuracy, the Kappa coefficient, and the receiver operating characteristics chart (ROC). The best result of classification was obtained by the probabilistic neural network of radial basis function (RBF) newpnn, with an overall accuracy of 88%, and a Kappa coefficient of 76%, showing it to be a very good classifier, and demonstrating the potential of this methodology to provide ecosystem services, particularly in anthropogenic areas in the Amazon that adopt agricultural systems with low carbon emissions.
Acesso aberto (Open Access)
Diagnóstico molecular e frequência de anticorpos anti-Leishmania infantum chagasi em cães do município de Belém, Pará
(2014-03) SCHWANKE, Katiane; SILVA, Aryane Maximina Melo da; OLIVEIRA, Adlilton Pacheco de; SILVA, Michele Bahia do Vale; SILVEIRA, Fernando Tobias; SCOFIELD, Alessandra; CAVALCANTE, Gustavo Góes
Visceral leishmaniasis is a disease whose etiological agent in Brazil is Leishmania infantum chagasi. Dogs are considered urban reservoirs of the disease, being an indicator of the human cases occurrence. The present study aimed to diagnose L. infantum chagasi infection in stray and owned dogs in Belém, Pará State, by polymerase chain reaction (PCR) and indirect immunofluorescence assay (IFA) using two different antigens. Venous blood samples from adult dogs, regardless of gender or breed, of different neighborhoods in Belém-PA, were collected in tubes with and without anticoagulant to obtain DNA and serum, respectively. These animals were divided into two groups: stray dogs captured by the Center for Zoonosis Control (Group A) and owned dogs (Group B). Sera were analyzed by IFA testing for IgG using two different antigens: 1) Bio-Manguinhos/Fiocruz antigen kit (Ag-PRO) containing promastigotes of Leishmania sp. (Complex Major-Like), 2) Instituto Evandro Chagas Antigen (Ag-AMA) consisting of amastigotes of L. infantum chagasi. The evaluation of the two antigens was performed considering positive the reactions above the 1:80 dilution. Already PCR was performed with DNA isolated from whole blood of animals and amplified with the primers RV1 and RV2. Of the 335 samples analyzed, 10.4% (35/335) were positive by IFA (Ag-PRO) and 0.9% (3/335) with the Ag-AMA. The distribution of positive samples is given as follows: Group A 14.8% (25/169) with Ag-PRO and 1.2% (2/169) with Ag-AMA; Group B 6% (10/166) with Ag-PRO and 0.6% (1/166) with Ag-AMA, being that all samples positive by IFA with Ag-AMA also reacted with Ag-PRO, and none of the samples detected DNA of L. infantum chagasi. The findings of this study indicate that Belém can still be considered non-endemic area for canine visceral leishmaniasis and the nature of the antigen influences the result of the IFA for the detection of anti-L. infantum chagasi antibodies in dogs, and the IFA using promastigotes of Leishmania major-like antigen should be used with caution as a confirmatory diagnostic on epidemiological studies in non-endemic areas.
Acesso aberto (Open Access)
Relative age effect on the reaction time of soccer players under 13 years old
(2015-06) PENNA, Eduardo Macedo; MELLO, Marco Túlio de; FERREIRA, Renato Melo; MORAES, Luiz Carlos Couto de Albuquerque; COSTA, Varley Teoldo da
The relative age effect, which is the advantage obtained by an athlete born closer to the beginning of the selection year, has been shown to be an important variable in the development of soccer players. This study aimed to evaluate the influence of the season of birth on the choice reaction time (CRT) of elite soccer athletes in the under 13 years of age category. Seventy-six athletes participated who were 13.36 ± 0.45 years old. The volunteers were tested with a CRT test, and the results were divided according to the semester of birth (S1 or S2). We conducted a t-test in order to compare the results between groups and a Pearson correlation analysis to verify the associations between variables. The results indicated no differences in reaction time (r= .033 and p = .772) or movement time (r = .0073 and p = .530). It was concluded that for this population, the season of birth does not influence the performance of the capabilities tested.
Acesso aberto (Open Access)
Expressão da E-caderina em carcinoma de células escamosas e no tumor de células basais de cães
(2011-09) JOÃO, Carolina Franchi; COSTA, Mirela Tinucci; CARDILLI, Diogo José; FARIA, Joice Lara Maia; MAGALHÃES, Geórgia Modé; ALESSI, Antonio Carlos
The cadherins are a group of cellular adhesion molecules that are expressed on the surface of all epidermic layer. The E-cadherin is the main cadherin involved in epithelial cellular adhesion; the decrease in its expression is related to the progression of some types of cancer, to its metastatic characteristics, and to the prognosis, specially carcinomas. The squamous cell carcinoma and the basal cells tumors are a malignant epithelial neoplasm which affects dogs. The goal of this study was to evaluate E-cadherin's expression in canine tissues that were classified as squamous cell carcinoma or basal cell tumor, and to find a correlation with the biological behavior of the tumors. The squamous cell carcinomas showed significantly decreased expression of the molecule compared to the basal cell tumors when evaluated by Fisher's test (P=0.0039). Also, the more differentiated neoplastic cells had a higher intensity of color than the less differentiated ones. In conclusion, it is suggested that the reduced expression of E-cadherin in skin tumors may indicate greater power infiltrative and consequently poor prognosis in dogs.
Acesso aberto (Open Access)
Development and assessment of a latex agglutination test based on recombinant MSP5 to detect antibodies against Anaplasma marginale in cattle
(2014) RAMOS, Carlos Alberto do Nascimento; ARAÚJO, Flábio Ribeiro de; SANTOS, Rafaelle Cunha dos; MELO, Elaine Silva de Pádua; SOUSA, Letícia Carvalho de; VIDAL, Carlos Eugênio Soto; GUERRA, Neurisvan Ramos Guerra; RAMOS, Rafael Antonio do Nascimento
The recombinant protein MSP5 has been established as an important antigen for serological diagnosis of Anaplasma marginale by enzyme-linked immunosorbent assay (ELISA). However, due to the high cost of specialized equipment, this technique is not accessible to all laboratories, especially in developing countries in areas where the disease is endemic. The present study describes the standardization of a latex agglutination test (LAT) to detect antibodies against A. marginale based on recombinant MSP5. Compared with indirect enzyme-linked immunosorbent assay (iELISA), the relative sensitivity and specificity of the LAT were 95.21% and 91.86% respectively, with an almost perfect agreement between tests (kappa index = 0.863). These results can be considered important for the serological diagnosis of A. marginale, as they indicate that the test represents a rapid and low cost alternative to ELISA.
Acesso aberto (Open Access)
Ethnoveterinary knowledge of the inhabitants of Marajó Island, Eastern Amazonia, Brazil
(Instituto Nacional de Pesquisas da Amazônia, 2011) MONTEIRO, Maria Vivina Barros; BEVILAQUA, Claudia Maria Leal; PALHA, Maria das Dores Correia; BRAGA, Roberta Rocha; SCHWANKE, Katiane; RODRIGUES, Silvane Tavares; LAMEIRA, Osmar Alves
There have been ethnoveterinary reports from around the world investigating plant usage in therapeutic protocols; however, there is no information regarding the ethnoveterinary practices in Brazilian Amazonia. The objective of this work was to register and document the ethnoveterinary knowledge of the inhabitants of the Island of Marajó, eastern Amazonia, Brazil. In the study, interviews were conducted with 50 individuals, with the application of semi-structured questionnaires that were quantitatively analyzed using descriptive statistic methods of frequency distribution. Use-value was calculated to determine the most important species. Samples of plants that were reported to have medicinal value were collected and identified by botanical classification. Fifty plants, distributed among 48 genera and 34 families, were indicated for 21 different medicinal uses. The family Asteraceae had the largest number of reported species; Carapa guianensis Aubl., Copaifera martii Hayne, Crescentia cujete L., Caesalpinia ferrea Mart., Chenopodium ambrosioides L., Jatropha curcas L. and Momordica charantia L. were species with highest use- value. The plant parts that were more commonly utilized for the preparation of ethnoveterinary medicines were the leaves (56%), bark (18%), roots (14%), seeds (14%) and fruit (8%). With regard to usage, tea was reported as a usage method by 56% of the informants; most preparations (90.9%) utilized only a single plant. In addition to medicinal plants, informants reported using products of animal and mineral origin. The present study contributed to the construction of an inventory of Marajó Island's ethnoveterinary plants, which might be the basis for future scientific validation studies.