Efeito protetor da flavana extraída da espécie Brosimum acutifolium contra danos causados por hipóxia em células retinianas: um estudo in vitro

Abstract

Ischemic stroke causes neuronal death due to excitotocity and oxidative stress. In this work, we investigate the neuroprotective effect of an amazon plant Brosimum acutifolium which is rich in flavanas with antioxidant potential, such as 4',7-dihydroxy-8-(3,3-dimethylallyl) flavana (brosimin b) (Bb), in a experimental model hypoxia in vitro. Neuroprotective effect of Bb was evaluated using cell cultures obtained from chick embryo retinas submitted to hypoxy by deprivation of oxygen and glucose. The antioxidant power of Bb was evaluated by kidnapping of 2,2-diphenyl-1-picryl-hydrazyl (DPPH) molecules. The neuroprotective activity was evaluated through the effects under cell viability, oxidative and antioxidative profile after 3, 6 and 24 hours after hypoxia trough oxygen reactive elements (O2-) analysis and endogenous antioxidant activity power of the catalase enzyme, respectively. We demonstrate that in vitro hypoxia causes time-dependent decreasing of cell viability due to the excessive production of O2-. On the other hands, the Bb treatment (10 μM) significantly preserved the cellular viability after 3 and 6 hours of in vitro hypoxia. This effect contributed to decrease the oxidative stress generated by productions of O2- during the 3 initial hours of hypoxia and also induced the increasing in the power activity of catalase enzyme in all the tested times. Thus, we show that Bb treatment has an antioxidant and neuroprotector effects due to contribute with antioxidant response during the neural hypoxia-induced oxidative stresse in vitro. These results suggest the use of Bb as a potential drug for Ischemic stroke treatment in vivo.