Use este identificador para citar ou linkar para este item:
https://repositorio.ufpa.br/jspui/handle/2011/6561
Tipo: | Artigo de Periódico |
Data do documento: | Dez-2014 |
Autor(es): | CARVALHO, Milene Alves de Figueiredo PAIVA, Renato STEIN, Vanessa Cristina HERRERA, Raírys Cravo PORTO, Jorge Marcelo Padovani VARGAS, Daiane Peixoto ALVES, Eduardo |
Título: | Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit |
Citar como: | CARVALHO, Milene Alves de Figueiredo et al. Induction and Morpho-Ultrastructural Analysis of Organogenic Calli of a Wild Passionfruit. Brazilian Archives of Biology and Technology, Curitiba, v. 57, n. 6, p. 851-859, dez. 2014. Disponível em: <http://www.scielo.br/pdf/babt/v57n6/1516-8913-babt-57-06-00851.pdf>. Acesso em: 20 abr. 2015. <http://dx.doi.org/10.1590/S1516-8913201402555>. |
Abstract: | This work studied a new protocol for organogenic calli induction and characterization of the morphology and ultrastructure of callogenesis in leaf explants of Passiflora gibertii N. E. Brown, a native passion fruit species from Brazil. Calli induction was performed in different growth conditions (light and dark), different MS medium salt concentrations (MS and MS half strength) and the presence or absence of coconut water. The leaf explants maintained in the dark were more responsive to bud formation. In order to reduce spending on in vitro culture, the most suitable induction medium for P. gibertii organogenesis could, therefore be the MS half strength salt concentration medium maintained in the dark. The addition of coconut water to the culture medium was essential for both calli induction and bud formation. The morphological and ultrastructural features of the organogenic calli were isodiametric cells, characterized by an organized cellular system, nucleus with prominent nucleoli, presence of starch grains and dense cytoplasm rich in endoplasmic reticulum. The scanning electron microscopy demonstrated that buds were present on these calli. |
Palavras-chave: | Fruta Passiflora Passiflora gibertii Microscopia ótica Cultura de tecidos Calogênese |
ISSN: | 1516-8913 |
Tipo de Acesso: | Acesso Aberto |
Aparece nas coleções: | Artigos Científicos - FCB/CALTA |
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Artigo_InductionMorphoUltrastructural.pdf | 864,43 kB | Adobe PDF | Visualizar/Abrir |
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