Assessing the genetic structure of Oryza glumaepatula populations with isozyme markers

Abstract

To assess the genetic diversity and genetic structure parameters, nine populations of Oryza glumaepatula from the Amazon biome, four from the Pantanal biome, and one collected at Rio Xingu, Mato Grosso, totaling 14 populations and 333 individuals were studied with isozyme markers. Six loci were evaluated showing a moderate allozyme variability (A = 1.21, P = 20.7%, Ho = 0.005, He = 0.060). The populations from the Pantanal biome showed higher diversity levels than the Amazon biome. High genetic differentiation among the populations, expected for self-fertilizing species, was observed (FST=0.763), with lower differentiation found among the Pantanal populations (FST=0.501). The average apparent outcrossing rate was higher for the Pantanal populations (ta = 0.092) than for the Amazonian populations (ta = 0.003), while the average for the 14 populations was 0.047, in accordance with a self-fertilization mating system.