Use este identificador para citar ou linkar para este item:
https://repositorio.ufpa.br/jspui/handle/2011/6591
Tipo: | Artigo de Periódico |
Data do documento: | Jun-2014 |
Autor(es): | ARAÚJO, Cristina Pires de OSÓRIO, Ana Luiza Alves Rosa JORGE, Klaudia dos Santos Gonçalves RAMOS, Carlos Alberto do Nascimento SOUZA FILHO, Antonio Francisco de VIDAL, Carlos Eugênio Soto VARGAS, Agueda Palmira Castagna de ROXO, Eliana ROCHA, Adalgiza da Silva SUFFYS, Philip Noel FONSECA JÚNIOR, Antônio Augusto SILVA, Marcio Roberto BARBOSA NETO, José Diomedes CERQUEIRA, Valíria Duarte ARAÚJO, Flábio Ribeiro de |
Título: | Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR |
Citar como: | ARAUJO, Cristina P. et al. Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR. Brazilian Journal of Microbiology, São Paulo, v. 45, n. 2, p. 633-640, abr./jun. 2014. Disponível em: <http://www.scielo.br/pdf/bjm/v45n2/35.pdf>. Acesso em: 24 abr. 2015. <http://dx.doi.org/10.1590/S1517-83822014000200035>. |
Abstract: | Post-mortem bacterial culture and specific biochemical tests are currently performed to characterize the etiologic agent of bovine tuberculosis. Cultures take up to 90 days to develop. A diagnosis by molecular tests such as PCR can provide fast and reliable results while significantly decreasing the time of confirmation. In the present study, a nested-PCR system, targeting rv2807, with conventional PCR followed by real-time PCR, was developed to detect Mycobacterium tuberculosis complex (MTC) organisms directly from bovine and bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other Actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. Regarding the analytical sensitivity, DNA of the M. bovis AN5 strain was detected up to 1.5 pg by nested-PCR, whereas DNA of M. tuberculosis H37Rv strain was detected up to 6.1 pg. The nested-PCR system showed 100% analytical specificity for MTC when tested with DNA of reference strains of non-tuberculous mycobacteria and closely-related Actinomycetales. A clinical sensitivity level of 76.7% was detected with tissues samples positive for MTC by means of the culture and conventional PCR. A clinical specificity of 100% was detected with DNA from tissue samples of cattle with negative results in the comparative intradermal tuberculin test. These cattle exhibited no visible lesions and were negative in the culture for MTC. The use of the nested-PCR assay to detect M. tuberculosis complex in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. |
Palavras-chave: | Bovino Bubalinos Tuberculose em animais Reação em cadeia da polimerase Tecido Inspeção sanitária |
ISSN: | 1517-8382 |
Tipo de Acesso: | Acesso Aberto |
Aparece nas coleções: | Artigos Científicos - FAMEVE/CCAST |
Arquivos associados a este item:
Arquivo | Descrição | Tamanho | Formato | |
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Artigo_DirectDetectionMycobacterium.pdf | 1,25 MB | Adobe PDF | Visualizar/Abrir |
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