Please use this identifier to cite or link to this item:
https://repositorio.ufpa.br/jspui/handle/2011/8103
metadata.dc.type: | Artigo de Periódico |
Issue Date: | Jun-2015 |
metadata.dc.creator: | LIMA, Eleonidas Moura LOPES, Otávio Sérgio SOARES, Leonardo Ferreira ARRUDA, Talitta Dantas GIGEK, Carolina Oliveira MELO, Cynthia Germoglio Farias SMITH, Marília de Arruda Cardoso OLIVEIRA, João Ricardo Gonçalves MEDEIROS, Arnaldo Correia de DELATORRE, Plínio RODRÍGUEZ BURBANO, Rommel Mario |
Title: | Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic |
Citation: | LIMA, Eleonidas Moura et al. Dideoxy single allele-specific PCR - DSASP new method to discrimination allelic. Brazilian Archives of Biology and Technology, Curitiba, v. 58, n. 3, p. 414-420, mai./jun. 2015. Disponível em: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000300414&lng=pt&nrm=iso>. Acesso em: 06 abr. 2017. <http://dx.doi.org/10.1590/S1516-8913201500434>. |
Abstract: | Gastric cancer (GC) is a multifactorial disease with a high mortality rate in Brazil and worldwide. This work aimed to evaluate single nucleotide polymorphisms (SNP) rs1695, in the Glutathione S-Transferase Pi (GSTP1) gene in GC samples by comparative analysis Specific PCR - ASP and Dideoxy Single Allele-Specific PCR - DSASP methods. The DSASP is the proposed new method for allelic discrimination. This work analyzed 60 GC samples, 26 diffuse and 34 intestinal types. The SNP rs1695 of the GSTP1 gene was significantly associated with GC analyzed by DSASP method (χ 2 = 9.7, P < 0.05). A comparative analysis of the data obtained from both methods did not differ significantly (χ 2 = 0.08, P > 0.05). These results suggest that the SNP rs1695 of the GSTP1 gene was a risk factor associated with gastric carcinogens is and the DSASP method was a new successfully low-cost strategy to study allelic discrimination. |
Keywords: | Câncer gástrico Genótipo Polimofismo de nucleotídeo Oncogenética Carcinoma gástrico |
metadata.dc.subject.cnpq: | CNPQ::CIENCIAS EXATAS E DA TERRA |
Series/Report no.: | Brazilian Archives of Biology and Technology |
ISSN: | 1678-4324 |
metadata.dc.publisher.country: | Brasil |
Publisher: | Universidade Federal do Pará |
metadata.dc.publisher.initials: | UFPA |
metadata.dc.rights: | Acesso Aberto |
Appears in Collections: | Artigos Científicos - ICB |
Files in This Item:
File | Description | Size | Format | |
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Artigo_DideoxySingleAllele.pdf | 258,32 kB | Adobe PDF | View/Open |
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