2014-04-282014-04-282014-02-13LIMA, Natália Pontes. Caracterização dos efeitos do extrato de folhas de Swietenia macrophylla em modelo experimental de doença de Parkinson. 2014. 68 f. Dissertação (Mestrado) - Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, 2014. Programa de Pós-Graduação em Neurociências e Biologia Celular.https://repositorio.ufpa.br/handle/2011/4906Previous studies indicate that the leaf extract of Swietenia macrophylla has abundant antioxidants in its chemical composition with neuroprotective effects in culture. One of the main mechanisms involved in neurodegeneration of Parkinson's disease (PD) is oxidative stress. Therefore, antioxidants are potential candidates for therapies aiming to slow the neurodegenerative process of the disease. This study aims to characterize the effects of the S.macrophylla leaf extract regrading the nigrostriatal degeneration and behavioral changes in mice exposed to a single unilateral injection of 6-OHDA in the striatum. Male mice were used, which underwent stereotactic surgery andwere injected 20 μg of 6-OHDA in the left striatum. The animals were divided into 4 groups according to the dose of mahogany extract administered. The extract was administered intraperitoneally in the first 7 days after injection of 6-OHDA at the doses of 0.0 (control) 0.5 (G1), 1.0 (0G2) and 5.0 mg/kg (G3). The control group (CG) received 0.9% saline (vehicle). Ambulation analysis in the open field was performed before, on the 7th and 21th days post-surgery and the number of apomorphine-induced rotations, in the 7th and 21th days after surgery. Evaluation of the neurodegeneration was performed by counting TH+ dopaminergic neurons in the substantia nigra by stereology. As a result, we found a statistically significant difference at day 21, where G2 and G3 showed a reduction in the ambulatory behavior in relation to G1 and GC; the latter two groups showed equivalent behavior. by, At day 21, G1 had significantly lower average apomorphine-induced rotations than GC, G2 and G3. In cell counts, G1 presented statistically significant reduction of the dopaminergic neurons loss compared to control. Thus, we conclude that the leaf extract of S.macrophylla in the concentration of 0.5 mg/kg provided neuroprotection for the nigrostriatal system neurodegeneration induced by 6-OHDA.porAcesso AbertoDoença de ParkinsonEpidemiologiaSwietenia macrophyllaMognoHidrodopaminasDissertaçãoCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA