2024-01-182024-01-182022-09-02SILVA, Danylla Cássia Sousa. Caracterização da gordura da amêndoa da gordura de cupuaçu (Theobroma grandiflorum S.) obtida por extração aquosa enzimática. Orientadora: Luiza Helena Meller da Silva. 2022. 90 f. Tese (Doutorado em Ciência e Tecnologia de Alimentos) - Universidade Federal do Pará, Instituto de Tecnologia, Belém, 2022. Disponível em: https://repositorio.ufpa.br/jspui/handle/2011/16181. Acesso em:.https://repositorio.ufpa.br/jspui/handle/2011/16181Aqueous enzymatic extraction is considered an emerging green technique that is being extensively investigated. In this work, cellulase, pectinase and protease enzymes are used in the aqueous extraction process of cupuassu fat, aiming to obtain a higher extraction yield. Different incubation systems were tested, at a temperature of 60 °C and following extraction kinetics (2 to 8 hours). The samples that obtained the highest fat extraction efficiencies were analyzed: aqueous phase, in terms of phenolic compounds and antioxidant activity, and oily phase (fats) characterized in terms of physicochemical properties, fatty acid profile, oxidative stability, thermal and bioactive compounds. The aqueous phase showed higher concentrations of phenolic compounds and antioxidant activity when compared to the oil phase. The fat samples showed approximate contents of monounsaturated and saturated fatty acids with higher concentrations of oleic and stearic, showed good oxidative stability (about 14 h) and thermal stability (close to 406 oC) and the best yield result (80.86 %) was achieved with the protease enzyme within 6 hours of incubation. The results indicated that cupuassu fats obtained by enzymatic aqueous extraction have potential for use in the food, pharmaceutical and cosmetics sectors.Acesso AbertoAttribution-NonCommercial-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nc-nd/3.0/br/Extração verdeEficiência de extraçãoTheobroma grandiflorum S.Sementes amazônicasQualidadeGreen extractionExtraction efficiencyTheobroma grandiflorum S.Amazonian seedsQualityTeseCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSPROPRIEDADES QUÍMICAS, BIOQUÍMICAS E MICROBIOLÓGICAS DOS ALIMENTOS E COMPOSTOS BIOATIVOSAPROVEITAMENTO DE RESÍDUOS AGROINDUSTRIAIS E DESENVOLVIMENTO DE PRODUTOSANÁLISES E DESENVOLVIMENTO DE PROCESSOS DE SEPARAÇÃO E TRANSFORMAÇÃO APLICADAS EM ALIMENTOSCIÊNCIA E TECNOLOGIA DE ALIMENTOS