2017-03-292017-03-292016-10-07GOBBO, Angélica Rita. Avaliação de biomarcadores sorológicos em um estudo de busca ativa de casos novos de hanseníase em área hiperendêmica. 2016. 48 f. Dissertação (Mestrado) – Universidade Federal do Pará, Instituto de Ciências Biológicas, Belém, 2016. Programa de Pós-Graduação em Neurociências e Biologia Celular.https://repositorio.ufpa.br/handle/2011/8055Leprosy is a cronic infection diasease clinically characterized by changes in tactile, thermal and painful sensitivity in skin and peripheral nerves. Due to the absence of laboratory diagnosis of leprosy, new tools that contribute for identification of cases are necessary for enable patient treatment before progression to physical disabilities. In this sense, the present study aimed evaluate serological biomarkers contribution for early diagnosis of leprosy. Was perfomed an active case finding study in Mosqueiro district, Belém – Pará. All individuals were clinically examined by experient leprologists doctors and than 5mL of peripheral blood were colleted for future titration of anti-ND-O-BSA, anti-LID-1 e anti-NDO-LID by ELISA. The action of active finding in Mosqueiro district diagnosed 104 new cases of leprosy between 895 subjects examined (11.6%), indicating a high hidden endemy that agree with the high seroprevalence between schoolchildren. Were observed a significant difference among patients with late or early diagnoses, mainly in multibacillary forms. All biomarkers tested showed promising results in detection of late cases, such as related in literature, however, for early cases those molecules identified correctly only 50% of patients. None of biomarker tested presented sufficient sensitivity to detect all leprosy patients, early or lately diagnosed. Besides, LID-1 molecule had evidenced a lower sensitivity for early cases, their high especificity and accuracy suggest their use as a potential tool for serological screening to identify assintomatic subjects with high risk of illness. Thus, we concludes that besides no biomarker had reveled utility as a serological diagnostic tool, the detection of anti-LID-1 presented a possible aplicability as a screening marker of subjects with increased risk to develop leprosy, contributing indirectly for leprosy diagnosis.Acesso AbertoHanseníaseBiomarcadoresDoenças endêmicasDoenças infecciosasDiagnósticoDissertaçãoCNPQ::CIENCIAS DA SAUDECNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIACNPQ::CIENCIAS BIOLOGICAS::BIOQUIMICA::BIOLOGIA MOLECULAR