2013-05-272013-05-272009SILVA, Dayse Danielle de Oliveira. Detecção in situ de citocinas de perfil TH1 e TH2 por imunoistoquímica e relação com a atividade de macrófagos nas formas polares da doença de Hansen. 2009. 100 f. Dissertação (Mestrado) - Universidade Federal do Pará, Núcleo de Medicina Tropical, Belém, 2009. Programa de Pós-Graduação em Doenças Tropicais.https://repositorio.ufpa.br/handle/2011/3910Leprosy is an infectious disease that has a slow and progressive evolution, with high incidence and prevalence in the states that compose the Legal Amazon. Considering the number of cases, the Pará state plays a prominent role in the national context. In order to evaluate the cytokine profile and the macrophages activity in the polar forms of leprosy and correlate with their histopathological aspects, a transversal study was performed, with a sample of 29 patients who had one of the polar forms of leprosy, namely tuberculoid or virchowian, from who skin biopsies of leprosy lesions were taken. It could be observed, through the analysis of this immunohistochemical material, that the studied groups showed no significant variation in the levels of IL-10, TGF-β, CD68, iNOS and Lysozyme. The levels of IL-4 were not expressive in the analyzed tissues and the levels of IFN-γ were higher in patients in the MHV group. In the virchowian pole, all cytokines tended towards a negative correlation with the macrophages activity. In the tuberculoid pole, the level of IL-10 showed a negative correlation with the levels of iNOS, IFN-γ and lysozyme, indicating a macrophage activity inhibition for this cytokine. It can be concluded that there is no difference in cytokine expression of TH1 and TH2 profile in polar forms of Hansen's disease and cytokines have a tendency to negative correlation with the activity of macrophages in MHV pole and a tendency to positive correlation in the pole MHT. Further research is needed to clarify why some populations have more susceptibility to leprosy than others. A similar study with a larger number of individuals may have a more significant outcome.porAcesso AbertoHanseníaseImunoistoquímicaDoenças transmissíveisCitocinasPará - EstadoAmazônia brasileiraDissertaçãoCNPQ::CIENCIAS BIOLOGICAS::IMUNOLOGIACNPQ::CIENCIAS DA SAUDE::MEDICINA::CLINICA MEDICA::DOENCAS INFECCIOSAS E PARASITARIAS