2014-12-182014-12-182013-11-29CHADA, Fabio José Garcia. Avaliação da atividade antimicrobiana de extratos de própolis em diferente maturação. 2013. 72 f. Dissertação (Mestrado) - Universidade Federal do Pará, Instituto de Ciências da Saúde, Belém, 2013. Programa de Pós-Graduação em Ciências Farmacêuticas.https://repositorio.ufpa.br/handle/2011/6172The discovery and synthesis of antimicrobial comprise an element of paramount importance to health, however some of these substances have become obsolete due to the emergence of resistant microorganisms conventional therapy. Within the forms of treatment, natural products are an inexhaustible source of substances, including propolis, which is known worldwide due to its antimicrobial activity. The objective of this study was the microbiological analysis of six propolis samples collected from regions Prudentópolis - PR , at different times of deposit hive, with up to 40 days of deposit (New Propolis) and another with over 180 days (Propolis old). The antimicrobial activity was observed against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Micrococcus luteus and Enterococcus faecalis through testing microdilution plate with colorimetric assay using resazurin. At the end of the tests gave values ranging from 0.38 to 0.68 mg/ml to MIC new propolis and 0.34 to 1.3 mg/ml for the old to the microorganisms S. aureus and M. luteus having values close to both of these as well as the CBM between 0.38 to 1.62 and from 0.67 to 2.6 mg/ml, respectively. Similarly, for E. faecalis MIC values between 0.76 and 2.73 mg/ml for the new propolis and 2.72 and 1.34 mg/ml of the propolis old were made, and MBC values of 1.50 to 3.07 and 2.68 to 3.11 mg/ml, respectively, for samples 1V, 2N and 5N 5V CBM not observed for the concentrations studied. Gram- negative microorganisms were not sensitive to propolis. We conclude that the new propolis showed better antimicrobial activity, especially against S. aureus and M. luteus. However, the data also show that the values of MIC and MBC were very close between the different propolis, which was not evident reasons why the old propolis is discarded.porAcesso AbertoPrópolisUso terapêuticoAvaliaçãoAtividade antimicrobianaPrudentópolis - PRParaná - EstadoDissertaçãoCNPQ::CIENCIAS DA SAUDE::FARMACIA::ANALISE TOXICOLOGICACNPQ::CIENCIAS DA SAUDE::FARMACIA::ANALISE E CONTROLE E MEDICAMENTOS